Anti-Human CD18 (ITGB2) – Dylight® 488

Anti-Human CD18 (ITGB2) – Dylight® 488

Product No.: C939

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Clone
IB4
Target
CD18
Formats AvailableView All
Product Type
Hybridoma Monoclonal Antibody
Alternate Names
Integrin beta-2, integrin beta chain, beta 2, ITGB2
Isotype
IgG2a κ
Applications
FA
,
FC
,
IF Microscopy

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Select Product Size
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Human peripheral blood lymphocytes
Product Concentration
0.2 mg/ml
Formulation
This DyLight® 488 conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
State of Matter
Liquid
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
This DyLight® 488 conjugate is stable when stored at 2-8°C. Do not freeze.
Regulatory Status
Research Use Only
Country of Origin
USA
Shipping
2-8°C Wet Ice
Excitation Laser
Blue Laser (493 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
FC,
IHC,
IP
Additional Applications Reported In Literature ?
IF Microscopy,
FA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
IB4 activity is directed against human CD18 and can also stain rabbit bone marrow leukocytes.
Background
Integrins are a large family of α/β heterodimeric transmembrane molecules that mediate adhesion, migration, cell survival, and cell differentiation1. The leukocytic cell-adhesion molecule (Leu-CAM) family has three members, LFA-1 (CD18/CD11a), CR3 (CD18/CD11b), and p150,95 (CD18/CD11c), all of which share the β chain CD182.

Murine (m) 1B4 was humanized (h) by the CDR-grafting method to create an anti-CD18 antibody with similar avidity, specificity, and efficacy as the murine antibody3. In rhesus monkeys, h1B4 is less immunogenic than m1B4 and elicits only anti-idiotypic antibodies. Additionally, h1B4 and m1B4 exhibit equivalent staining specificity and intensity in rabbit bone marrow leukocytes.

For humanization, human VH and VL FR were chosen based on high identity with their corresponding m1B4 FR3. Once grafted with the m1B4 CDR, the human VH and VL FR were expressed as fusion proteins with the IgG4 and Cκ human constant regions, respectively.

The h1B4 monoclonal antibody has been used in multiple studies of adhesion in different model systems4, 5, 6 and to measure the expression of CD18 in B-ALL cell lines7.
Antigen Distribution
Depending on its heterodimeric configuration, CD18 is expressed in leukocytes, monocytes, macrophages, some lymphocytes, polymorphonuclear lymphocytes (PMNs), natural killer cells, neutrophils, granulocytes, certain lymphocytic tumor cell lines, macrophage foam cells, activated platelets, and splenic red pulp macrophages.

Antigen Details

Ligand/Receptor
Receptor
NCBI Gene Bank ID
UniProt.org
Research Area
Cell Adhesion
.
Cell Biology
.
Immunology
.
Signal Transduction

References & Citations

1. Luo BH, Carman CV, Springer TA. Annu Rev Immunol. 25:619-647. 2007.
2. Sanchez-Madrid F, Nagy JA, Robbins E, et al. J Exp Med. 158(6):1785-1803. 1983.
3. Singer II, Kawka DW, DeMartino JA, et al. J Immunol. 150(7):2844-2857. 1993.
4. Hanlon WA, Stolk J, Davies P, et al. J Leukoc Biol. 50(1):43-48. 1991.
5. Seth R, Salcedo R, Patarroyo M, et al. FEBS Lett. 282(1):193-196. 1991.
6. McDonald RJ, St George JA, Pan LC, et al. Inflammation. 17(2):145-151. 1993.
7. Velázquez-Avila M, Balandrán JC, Ramírez-Ramírez D, et al. Leukemia. 33(6):1337-1348. 2019.
FA
Flow Cytometry
IF Microscopy

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.