Anti-Human CD195 (CCR5) [Clone HEK/1/85a/7a] – Purified in vivo GOLD™ Functional Grade

The clone HEK/1/85a/7a is specifically designed and used for in vivo mouse studies as a functional grade antibody targeting human CD195 (CCR5). This antibody is manufactured with special considerations for in vivo applications that distinguish it from standard research antibodies.

In Vivo Functional Grade Specifications

The HEK/1/85a/7a clone is available as an "in vivo GOLD™ Functional Grade" antibody, which indicates it has been specifically purified and tested for use in live animal studies. This functional grade designation ensures the antibody meets the stringent requirements necessary for in vivo applications, including appropriate purity levels and reduced endotoxin content.

Low Endotoxin Manufacturing

For in vivo mouse studies, this antibody is manufactured with low endotoxin levels (less than 1.0 EU/mg), which is crucial for preventing inflammatory responses that could confound experimental results. Some manufacturers also offer ultra-low endotoxin versions with even lower endotoxin content (<0.5 EU/mg) at higher purity levels (98% versus 95%) for more sensitive in vivo applications.

Species Reactivity Considerations

An important limitation for in vivo mouse studies is that the HEK/1/85a/7a antibody shows species-specific reactivity. The antibody recognizes human CCR5 but has limited cross-reactivity with mouse CCR5. This means that for standard mouse studies, researchers would typically need to use humanized mouse models that express human CCR5 rather than the native mouse protein, or the antibody would be used in xenograft models where human cells are transplanted into immunocompromised mice.

Dosing and Administration

The antibody is available in bulk quantities suitable for in vivo studies, including 5mg, 25mg, 50mg, and 100mg sizes, with multi-gram quantities available upon request. The specific dosing would depend on the experimental design, mouse strain, and study objectives, though exact protocols would need to be optimized for each specific research application.

The rat IgG2b isotype of this antibody is well-suited for in vivo applications as it typically has good stability and appropriate pharmacokinetic properties for mouse studies.

Storage Temperature for Clone HEK/1/85a/7a

For the sterile packaged (bulk/lyophilized, unopened) clone HEK/1/85a/7a (anti-human CD195/CCR5 antibody):

• Short-Term Storage: Store the purified antibody solution (typically in buffer) between 2°C and 8°C.
• Long-Term Storage: For extended shelf life, aliquot the antibody into working volumes (aliquots) without diluting, and store at –20°C or –80°C. This prevents degradation and maintains stability over months to years.
• Avoid Repeated Freeze-Thaw: Consistent freeze-thaw cycles should be avoided, as they can reduce antibody efficacy.

Additional Details

• Packaging Variants: If the antibody comes in a PBS-based buffer with a preservative (such as 0.02% sodium azide), some vendors recommend –15°C to –25°C for long-term storage. Always check the specific product datasheet for exact instructions.
• Clarification on Clone: Clone HEK/1/85a is the same as clone HEK/1/85a/7a.
• Light Sensitivity: Some formats may require protection from light during storage.

Summary Table

Always refer to the manufacturer’s datasheet for the specific product lot, as storage recommendations may vary slightly depending on formulation and packaging.

The antibody HEK/1/85a/7a is an anti-CCR5 antibody widely used for detecting the chemokine receptor CCR5 in research, especially in studies involving receptor pharmacology or immunology. In the literature, it is often used alongside other antibodies and proteins in experiments involving cell lines expressing or lacking CCR5 or related chemokine receptors.

Commonly used antibodies or proteins with HEK/1/85a/7a:

• Anti-CCR5 antibodies such as 1/74/3j, which targets the CCR5 N-terminal peptide, provide comparative or complementary CCR5 detection.
• Anti-CD4 antibodies are commonly used with anti-CCR5 antibodies to characterize receptor expression or to study co-receptor functions in immunology, particularly in HIV research.
• Anti-G protein antibodies may be employed where downstream signaling or G-protein coupling of CCR5 is of interest.

Additional context:

• In studies using HEK293 or HEK293T cells (which are commonly used for transfection and expression of recombinant proteins or receptors), antibodies that target human markers or other introduced genes/proteins may be used alongside CCR5 antibodies, depending on the experimental setup.
• Other proteins relevant in CCR5- or GPCR-related research include chemokines like CCL3 (the natural ligand for CCR5), which might be used to stimulate or assay receptor activity.

Researchers may choose further antibodies based on the experimental context (e.g., anti-MICA for immunology, various anti-GPCR/ligand antibodies for receptor pharmacology). However, for studies specifically referencing HEK/1/85a/7a, anti-CCR5, anti-CD4, and anti-G protein antibodies are the most directly and frequently mentioned counterparts in the literature.

Key Findings from Citations of Clone HEK/1/85a/7a

Clone Identification and Applications

• Clone HEK/1/85a/7a is an antibody targeting the human CD195 (CCR5) surface receptor, also known as C-C chemokine receptor type 5.
• This clone is recognized to bind the same epitope as HEK/1/85a and HEK/1/85a/7a, indicating that, functionally, these two clones are interchangeable.
• The primary use of this clone in the literature and commercial datasheets is for the detection and analysis of CCR5 expression in human cells, with reported applications including immunofluorescence microscopy.

Role in Scientific Research

• CCR5 (CD195) is a key receptor involved in immune system signaling, especially in the context of HIV infection, as CCR5 is a major co-receptor for HIV-1 viral entry into cells.
• The HEK/1/85a/7a clone has been cited in research involving phenotypic characterization of immune cells, particularly in studies of HIV persistence and immune evasion. For example, in studies of HIV-1 reservoir cells, CCR5 (detected with this clone) was one of several surface markers used to profile the immune phenotype of infected cell populations, alongside markers such as PD1, TIGIT, and KLRG1.
• However, there is no evidence in the search results that clone HEK/1/85a/7a itself is specifically cited for novel scientific findings; rather, it is primarily a tool for CCR5 detection in experimental contexts.

Summary Table: Key Points

Conclusion

The HEK/1/85a/7a clone is a well-established tool for detecting human CCR5, with confirmed equivalence to HEK/1/85a. It is widely used in immunology and virology research, particularly for studying HIV infection and immune cell phenotypes, but there is no evidence in the available citations that it is directly associated with a specific scientific breakthrough or novel biological insight. Its value lies in its reliability for CCR5 detection in experimental systems.