Anti-Human Histone H3 Nonmethyl (Lys9) – Purified No Carrier Protein

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.

Purified antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

This antibody may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.

9B1-2G6 activity is directed against unmethylated human histone H3 (Lys9) when Ser10 is phosphorylated (H3K9me0). 9B1-2G6 also detects mono-/di-methyl-Histone H3 (Lys4), Acetyl-Histone H3 (Lys14), H3K4me3, Histone H3 K14ac, Histone H3K4ac, and Histone H3 S10ph.

Nucleosomes are the basic building blocks of chromatin¹. Nucleosomes are composed of ~147 bp of DNA wrapped around a histone octamer. This octamer is composed of a central (H3-H4) tetramer that is flanked by two H2A-H2B dimers. Histones H2B and H4 are relatively conserved, but many variants of H2A and H3 exist. H3 variants in humans include H3.1, H3.2, H3.3, H3t/H3.4, H3.5, H3.Y, H3.X, CENP-A, H3.6, H3.7 and H3.8. Both H3.1 and H3.2, which differ by a single amino acid at residue 96, are expressed in S-phase during replication. In contrast, H3.3 is expressed throughout interphase and is enriched over actively transcribed genes and repetitive DNA elements. The other variants are less studied, but all histones are heavily regulated at transcriptional and post-translational levels, including methylation, acetylation, and phosphorylation. Histone modification status has a direct biophysical effect on gene expression and chromatin structure. Lysines may be unmethylated (me0), monomethylated (me1), demethylated (me2), and trimethylated (me3), each with its own regulatory effects.

9B1-2G6 was raised against a synthetic, branched, KLH-conjugated peptide corresponding to human unmethylated Histone H3 amino acids surrounding Lys9. 9B1-2G6 targets H3 when unmethylated (me0) at Lys9. This antibody is also known as the Histone H3K9me0 antibody.

H3 is expressed in nucleosomes.

1 Scott WA, Campos EI. Front Cell Dev Biol. 8:701. 2020.
2 Longarini EJ, Dauben H, Locatelli C, et al. Mol Cell. 83(10):1743-1760.e11. 2023.