Anti-Human IκB Kinase Alpha (CT-2) (IKKα)

Anti-Human IκB Kinase Alpha (CT-2) (IKKα)

Product No.: I-300

[product_table name="All Top" skus="I-300"]

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Target
IκB Kinase Alpha
Product Type
Polyclonal Antibody
Alternate Names
IKK-1: Inhibitor of Nuclear Factor Kappa-B Kinase Alpha Subunit, NFκB Inhibitor Kinase Alpha
Applications
IHC FFPE
,
WB

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Data

I-300 Western Blow
I-300 IHC HeLa Cells
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Rabbit
Immunogen
PN:I-314
Product Concentration
0.5 mg/ml
Formulation
This polyclonal antibody is formulated in phosphate buffered saline (PBS) pH 7.4 containing 0.02% sodium azide as a preservative.
Storage and Handling
This polyclonal antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at –20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Anti-Human IκB Kinase Alpha (IKKa) recognizes an epitope near the C-terminus of Human IKKa. This polyclonal antibody was purified using affinity chromatography.
Background
Nuclear factor κ B (NF-κB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-κB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFα and bacteria product LPS. NF-κB is associated with IkB proteins in the cell cytoplasm, which inhibit NF-κB activity. The long-sought IκB kinase (IKK), which phosphorylates IκB, and mediates IκB degradation and NF-κB activation, was recently identified by several laboratories.1-4 IKK is a serine protein kinase, and the IKK complex contains α and β subunits (IKKα and IKKΒ). IKKα and IKKb interact with each other and both are essential for the NF-κB activation. IKKα specifically phosphorylates IκB-Α.IKKα is expressed in a variety of human tissues.

Antigen Details

References & Citations

1. DiDonato, JA. et al. (1997) Nature 388:548
2. Regnier, CH. et al. (1997) Cell 90:373
3. Zandi, E. et al. (1997) Cell 91:243
4. Woronicz, JD. et al. (1997) Science 278:866
IHC FFPE
General Western Blot Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.