Anti-Human IL-18 BPa
Anti-Human IL-18 BPa
Product No.: I-622
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Clone 136033 Target IL-18 BPa Formats AvailableView All Product Type Monoclonal Antibody Alternate Names Interleukin-18 Binding Protien a Isotype IgG1 Applications WB |
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Antibody DetailsProduct DetailsReactive Species Human Host Species Mouse Immunogen Purified Recombinant IL-18 BPa >98% Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830744 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human IL-18 BPa this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:M114 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-18 BPa is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-622. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Mouse Anti-Human Interleukin 18 Binding Protien a (IL-18 BPa) (Clone 136033) recognizes an epitope on Human IL-18 BPa. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background IL-18-binding protein (IL-18BP) is a secreted glycoprotein that functions as a natural anti-inflammatory and immunosuppressive molecule neutralizing the effects of high IL18 levels during inflammation.1 There are four variants of IL-18BP and IL18BPa is the predominant form showing the highest affinity towards IL18.1, 2 IL-18 BPa has the potential to amplify anti-inflammatory actions of immunosuppressive drugs, and thus may prove to be a valuable novel pharmacological component in the treatment of human autoimmune diseases.3 PubMed References & Citations1. Novick, D. et al. (1999) Immunity 10: 127 2. Kim, SH. et al. (2000) Proceedings of the National Acad. of Sci. (USA) 197: 1190 3. Heiko, M. et al. (2003) Biochemical pharmacology 66: 505 Technical Protocols Certificate of Analysis |
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