Anti-Human IL-18 BPa – Biotin
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Antibody DetailsProduct DetailsReactive Species Human Host Species Goat Immunogen Purified Recombinant Human IL-18 BPa (Accession # O95998) Formulation This biotinylated antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 50 µg of bovine serum albumin per µg of antibody with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized, biotinylated antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for up to twelve months from date of receipt. The reconstituted biotin conjugate can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted conjugate, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830873 Applications and Recommended Usage? Quality Tested by Leinco Western Blotting: To detect Human IL-18 BPa this biotin conjugate can be used at a concentration of 0.1 - 0.2 µg/ml. This biotin conjugate should be used in conjunction with compatible second-step reagents such as PN:A106 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-18 BPa is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:I-546. ELISA Sandwich Assay: This antibody can be used as the detection antibody in a sandwich ELISA at a concentration of approximately 0.1-0.4 µg/mL. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Goat Anti-Human Interleukin 18 Binding Protien a (IL-18 BPa) recognizes Human IL-18 BPa. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background IL-18-binding protein (IL-18BP) is a secreted glycoprotein that functions as a natural anti-inflammatory and immunosuppressive molecule neutralizing the effects of high IL18 levels during inflammation.1 There are four variants of IL-18BP and IL18BPa is the predominant form showing the highest affinity towards IL18.1, 2 IL-18 BPa has the potential to amplify anti-inflammatory actions of immunosuppressive drugs, and thus may prove to be a valuable novel pharmacological component in the treatment of human autoimmune diseases.3 PubMed NCBI Gene Bank ID UniProt.org References & Citations1. Novick, D. et al. (1999) Immunity 10: 127 2. Kim, SH. et al. (2000) Proceedings of the National Acad. of Sci. (USA) 197: 1190 3. Heiko, M. et al. (2003) Biochemical pharmacology 66: 505 Technical ProtocolsELISA Det  Certificate of Analysis |
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