Anti-Human IL-20 Rα

Product No.: I-603


Target IL-20 R alpha Product Type Polyclonal Antibody Alternate Names Interleukin-20 Receptor Alpha, FLJ40993, IL-20R1, ZCYTOR7 Applications CyTOF® , FC , WB


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Antibody Details Product Details Reactive Species Human Host Species Goat Immunogen NS0 - derived human IL-20 Rα extracellular domain Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present. State of Matter Lyophilized Storage and Handling The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830726 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IL-20 Rα. A suggested method would be to stain cells expressing IL-20 Rα with 2.5 µg/10⁶ cells in a total staining volume of 100 µl followed by PN:G641. Western Blotting: To detect Human IL-20 Rα this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-20 Rα is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. Additional Applications Reported In Literature ? CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. Description Description Specificity Goat Anti-Human Interleukin-20 Receptor Alpha (IL-20 Rα) recognizes Human IL-20 Rα. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research. Background Interleukin 20 receptor alpha (IL-20RA) is a subunit for the interleukin-20 receptor that is expressed by endothelial cells lining the intimal microvessels, vasa vasorum, but rarely in nonatherosclerotic arteries.¹ IL-20RA is a class II cytokine receptor that has many physiological functions such as apoptosis, hematopoiesis, and anti-tumor activities. IL-20RA functions as an aggravating factor in promoting calcium deposition in kidney of mice.² PubMed IL-20 R alpha NCBI Gene Bank ID 53832 UniProt.org More Information on Uniprot.org References & Citations 1. Hackstein, H. et al. (2003) Genes Immun. 4:228 2. Wei, CC. and Chang, MS. (2008) Genes Immun. 9(8):668-79 View product citations for antibody I-603 on CiteAb Technical Protocols CyTOF® Certificate of Analysis Request COA

Antibody Details

Product Details

Reactive Species

Human

Host Species

Goat

Immunogen

NS0 - derived human IL-20 Rα extracellular domain

Endotoxin Level

<0.1 EU/µg as determined by the LAL method

Formulation

This antigen affinity purified polyclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 containing 5.0% w/v trehalose with no calcium, magnesium, or preservatives present.

State of Matter

Lyophilized

Storage and Handling

The lyophilized antigen affinity purified polyclonal antibody can be stored desiccated at -20°C to -70°C for twelve months from date of receipt. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. No detectable loss of activity was observed after six months.

Country of Origin

USA

Shipping

Next Day Ambient

RRIDAB_2830726

Applications and Recommended Usage?
Quality Tested by Leinco

Flow Cytometry: It is recommended to use the indirect method for signal enhancement when enumerating cells expressing IL-20 Rα. A suggested method would be to stain cells expressing IL-20 Rα with 2.5 µg/10⁶ cells in a total staining volume of 100 µl followed by PN:G641.
Western Blotting: To detect Human IL-20 Rα this polyclonal antibody can be used at a concentration of 0.1-0.2 µg/ml. This polyclonal antibody should be used in conjunction with compatible second-step reagents such as PN:G505 and a chromogenic substrate such as PN:T343. The detection limit for Human IL-20 Rα is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used.

Additional Applications Reported In Literature ?

CyTOF-ready: Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity

Goat Anti-Human Interleukin-20 Receptor Alpha (IL-20 Rα) recognizes Human IL-20 Rα. This antigen affinity purified polyclonal antibody was purified using a proprietary chromatographic technique that includes covalently immobilizing the antigen proteins or peptides to agarose based beads. This purification method enhances specificity, reduces nonspecific binding of extraneous IgG and provides you with the most reliable reagent available for your early discovery research.

Background

Interleukin 20 receptor alpha (IL-20RA) is a subunit for the interleukin-20 receptor that is expressed by endothelial cells lining the intimal microvessels, vasa vasorum, but rarely in nonatherosclerotic arteries.¹ IL-20RA is a class II cytokine receptor that has many physiological functions such as apoptosis, hematopoiesis, and anti-tumor activities. IL-20RA functions as an aggravating factor in promoting calcium deposition in kidney of mice.²

PubMed

IL-20 R alpha

NCBI Gene Bank ID

53832

UniProt.org

More Information on Uniprot.org