Anti-Human MMP-14 (MT1-MMP)

Pricing & Details

Product No.M1089
Clone
M43.266
Protein
MMP-14
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
MT1-MMP
Isotype
Mouse
IgG1 κ
Applications
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
M1089-0.2 mg
0.2 mg
$252.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
A synthetic peptide corresponding to amino acids 160 to 173 of human MMP-14
Product Concentration
1.0 mg/ml
Formulation
This purified antibody is formulated with no carrier proteins or preservatives added in 0.01 M phosphate buffered saline (PBS) pH 7.4, 150 mM NaCl.
Storage and Handling
The antibody should be stored at -20°C. Avoid repeated freezing and thawing. This product does not contain preservatives.
Country of Origin
USA
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Anti-Human MMP-14 (clone M43.266) was produced in mice using a synthetic peptide corresponding to amino acids 160 to 173 of human MMP-14.
Background
Matrix metalloproteinases (MMPs) are a family of enzymes that are responsible for the degradation of extracellular matrix components. Of the eleven proteins reported to date, ten are normally found as soluble molecules. The eleventh, and newest of these proteins, has been named MT-MMP for membrane bound MMP. MT-MMP contains a C-terminal transmembrane domain which allows it to localize to the cell surface. It is also unique from the other members of the MMP family in that it contains an 8 amino acid insert in the catalytic domain. The protein is encoded by a 4.5 kb mRNA species giving rise to a protein with a molecular weight of 60-66 kDa by SDS-PAGE. MT-MMP is responsible for cleaving gelatinase A (MMP-2, 72 kDa Type IV collagenase) to the active form. Evidence exists to suggest that MT-MMP itself requires an activation step which is most likely the result of activity of the membrane plasmin cascade. MT-MMP functions by binding TIMP-2 and then the COOH terminal end of MMP-2 resulting in a 105 kDa trimer which effects the cleavage of pro-MMP-2 to the biologically active form. The order of the binding of pro-MMP-2 and TIMP-2 to MT-MMP is critical as TIMP-2 will also inhibit the activity of MMP-2 when present in a soluble form. Since its discovery, a second form of MT-MMP has been identified resulting in a change in the name of the original protein to MT1-MMP.

Antigen Details

Protein
PubMed
Research Area
Apoptosis
.
Cell Biology
.
Immunology

References & Citations

1. Strongin, A. Y. et al. (1995) J. Biol. Chem. 270:5331
2. Takino, T. et al. (1995) J. Biol. Chem. 270:23013
3. Sato, H. et al. (1994) Nature 370:61
4. Cottam, DW. et al. (1993) Intl. J. Oncol. 2:861
5. Stetler-Stevenson, WG. et al. (1993) FASEB J. 7:1434
6. Fridman, R. et al. (1992) J. Biol. Chem. 267:15389
7. Woessner, JF. (1991) FASEB J. 5:2145
8. Goldberg, GI. et al. (1989) Proc. Natl. Acad. Sci. 86:8207
General Western Blot Protocol

Formats Available

Prod No.
Description
M1089
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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