Anti-Human/Mouse CDCP1 (CD318) – Purified in vivo GOLD™ Functional Grade

Common In Vivo Applications of Clone 9A2 in Mice

Clone 9A2 is a monoclonal antibody primarily used in in vivo mouse studies to investigate the biology of CDCP1 (CUB domain-containing protein 1, also known as CD318). While detailed protocols or specific experimental outcomes are not elaborated in the provided search results, the general applications can be inferred from the nature of monoclonal antibody use in biomedical research.

Typical Experimental Uses

• Direct Antibody Administration: Clone 9A2 is administered to mice to manipulate or block CDCP1 function in vivo, allowing researchers to study the role of CDCP1 in physiological or pathological processes.
• Immunofluorescent Labeling: The antibody can be used to visualize CDCP1 expression in tissues or specific cell populations within live mice, aiding in the spatial and temporal analysis of CDCP1 distribution during development, disease, or treatment.
• Functional Assays: By targeting CDCP1 with clone 9A2, researchers can perform in vivo functional assays to assess the impact of CDCP1 modulation on cellular behaviors such as migration, adhesion, or signaling in relevant disease models (e.g., cancer, metastasis, or wound healing).

Context of CDCP1 Studies

CDCP1 is a transmembrane glycoprotein implicated in processes such as cancer metastasis, cell adhesion, and signaling. Thus, in vivo applications of clone 9A2 are likely focused on understanding these roles in mouse models of disease, particularly in oncology where CDCP1 has been linked to tumor progression and metastasis.

Summary Table

Conclusion

Clone 9A2 is used in mice mainly to probe the in vivo functions of CDCP1 through targeted antibody intervention, visualization, and functional assessment, with a focus on understanding its role in disease processes such as cancer. Specific experimental designs (e.g., tumor xenograft models, metastasis assays) would depend on the research question, but these represent the core in vivo applications for this clone.

Based on available literature, there are at least two different antibodies named 9A2, each with distinct targets and research contexts. Below, I clarify which proteins or antibodies are commonly used alongside each 9A2, depending on the target.

In the Context of Oct-2 (POU2F2) Research

The 9A2 clone (e.g., ab243153, ab252246) is a well-characterized monoclonal rat antibody targeting the Oct-2 transcription factor, which is expressed in B lymphocytes and some other cell types. In the literature and laboratory protocols for Oct-2 research, 9A2 is typically used alone for Western blot (WB), immunohistochemistry (IHC), and flow cytometry (intracellular) to detect Oct-2 protein. There is no strong evidence from the available sources that 9A2 is routinely paired with other specific antibodies (aside from standard secondary antibodies) in a co-staining or co-immunoprecipitation context for Oct-2 studies.

Examples of Common Applications

• Western Blot: 9A2 is used to detect Oct-2 in lysates from human (e.g., Ramos, Raji, Daudi cells), mouse (e.g., A20 cells), and tissue samples (human tonsil, mouse spleen).
• Flow Cytometry: Intracellular staining of Oct-2 in fixed, permeabilized cells, often using a fluorescent secondary antibody (e.g., Goat Anti-Rat IgG Alexa Fluor® 488).
• IHC-P: Immunohistochemistry on paraffin-embedded tissues.

Typical Secondary and Control Antibodies

• Goat Anti-Rat IgG (HRP, ab205720): For WB detection.
• Goat Anti-Rat IgG (Alexa Fluor® 488, ab150157): For flow cytometry.
• Isotype control: Rat monoclonal IgG (unspecified clone) is used as a negative control in flow cytometry experiments.

There is no indication from the provided sources that 9A2 is routinely used in combination with other primary antibodies targeting different proteins in the same experiment, though it is standard to use it with appropriate secondary antibodies and isotype controls.

In the Context of VASP Research

Another 9A2 clone exists (e.g., #3132, Cell Signaling Technology), which is a rabbit monoclonal antibody targeting Vasodilator-Stimulated Phosphoprotein (VASP). This antibody is used in studies of cytoskeletal regulation, cell migration, and signaling pathways.

Commonly Used Proteins/Pathways Alongside VASP 9A2

• Actin: Studies of cytoskeletal dynamics often pair anti-VASP with anti-actin antibodies, given VASP's role in actin reorganization.
• Other Actin-Binding Proteins: In kits and multiplex assays, VASP 9A2 may be used alongside antibodies to other actin regulators, such as cofilin, profilin, or Arp2/3 complex components.

Typical Secondary Antibodies

• Anti-Rabbit IgG (HRP or fluorescent conjugates): For detection in WB, IHC, or IF, depending on the host species of the primary.
• Isotype control: Rabbit monoclonal IgG as a negative control.

Again, there is no specific mention of routine pairings with other primary antibodies in the available literature, but it is common in cytoskeletal research to use VASP 9A2 alongside antibodies to other cytoskeletal markers in multiplex assays.

Summary Table

Key Points

• Oct-2 9A2 is used to detect the Oct-2 transcription factor, typically with appropriate secondary antibodies and negative controls, but not in combination with other primary antibodies against distinct targets in the literature reviewed here.
• VASP 9A2 is used in cytoskeletal studies, often in parallel with anti-actin or related antibodies, especially in multiplex assays investigating cytoskeletal dynamics.
• There is no overlap or cross-reactivity between these two 9A2 clones; they recognize entirely different proteins (Oct-2 vs. VASP) and are used in separate research fields.

If your interest is in a specific 9A2 antibody (Oct-2 or VASP), please clarify the context, as the proteins and antibodies commonly used in conjunction depend entirely on the target and research area.

Based on the available search results, there is no substantial scientific literature specifically discussing clone 9A2 with clear, documented findings. The search results reveal that clone 9A2 appears in different contexts but lacks comprehensive citation data or key research findings.

Available Context for Clone 9A2

The limited information suggests that 9A2 is used as an antibody clone designation in immunological research, particularly:

Anti-Oct-2 9A2 Clone: This clone is primarily used in B-cell research for detecting the Oct-2 protein. The standard detection method involves using goat anti-rat IgG (either HRP-conjugated or fluorescently labeled) as the secondary antibody.

VASP (9A2): This represents a distinct antibody clone that targets a different protein and should not be confused with the anti-Oct-2 9A2 clone.

Absence of Citations

The search results explicitly state that "there is no citation to clone 9A2 in the provided scientific literature search results, nor is there any mention of such a clone in the available excerpts". The available research discusses other topics entirely, such as clonal hematopoiesis in cardiovascular disease and recombinant enzyme characterization, but these do not reference clone 9A2.

Context-Dependent Usage

The 9A2 identifier appears to be used across multiple research contexts—including HIV research, B-cell biology, and cytoskeleton regulation—but the associated proteins and antibodies differ depending on the specific field. Without additional context about which specific research area or application you're interested in, it's not possible to provide comprehensive findings about this clone's scientific impact or citations.

To obtain meaningful information about clone 9A2, you would need to specify the research domain, target protein, or application area of interest.

Dosing Regimens for Clone 9A2 (Anti-Human/Mouse CDCP1/CD318) Across Mouse Models

Clone 9A2 targets the CDCP1 (CD318) antigen and is used in vivo for experimental purposes, particularly in mouse models. However, the available literature does not specify substantial variations in dosing regimens for clone 9A2 across different mouse disease models. Most information is provided as a general, model-agnostic guidance.

Standard Dose Range and Administration

• Dose Range: 100–500 µg per mouse per administration.
• Route: Intraperitoneal (IP) injection is standard.
• Frequency: Dosing schedules are typically 2–3 times per week, or every 3–4 days, depending on the specific clone and application.
• Model Specificity: No evidence in the available literature indicates model-specific adjustments (e.g., cancer, infection, immunology) for clone 9A2; the recommended range appears to be broadly applicable across experimental setups.

Comparison to Other Antibody Clones

For context, other commonly used antibody clones (e.g., anti-PD-1, anti-PD-L1, anti-CTLA-4) have dosing regimens that are also generally consistent across mouse models, with minor adjustments based on the specific therapeutic goal or combination therapy but not fundamentally different between, for example, tumor types or infection models. For clone 9A2, no such detailed, model-specific guidance is documented in the searched literature.

Summary Table

Key Points

• No evidence from the literature suggests that clone 9A2 dosing varies significantly between different mouse models.
• The 100–500 µg range and IP administration appear to be standard, with dosing frequency adjusted based on experimental needs rather than model type.
• For specific applications (e.g., combination therapy, unique disease contexts), researchers should consult primary literature or perform pilot studies to optimize dosing, but no published data indicate a need for model-specific regimens at this time.

In summary, clone 9A2 dosing in mice is guided by a general protocol (100–500 µg IP, 2–3 times per week or every 3–4 days), without documented variations tailored to specific mouse models in the available sources. For precise applications, empirical optimization is recommended, as with most antibody therapies in preclinical research.