Anti-Human/Mouse CDCP1 (CD318) – Purified

Anti-Human/Mouse CDCP1 (CD318) – Purified

Product No.: C9110

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Clone
9A2
Target
CD318
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CDCP1; CD318; TRASK; SIMA135
Isotype
IgG2b κ
Applications
FC

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Product Size
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Antibody Details

Product Details

Reactive Species
Human/Mouse
Host Species
Mouse
Immunogen
His-tagged recombinant fragment from the internal region of human CUB domain-containing protein 1
Product Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This purified antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this 9A2 antibody for staining cells in flow cytometry is 1 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 9A2 recognizes an epitope within the extracellular domain of human/mouse CDCP1.
Antigen Distribution
CDCP1 is widely expressed in the esophagus, skin, colon, duodenum, stomach and other tissues.
Background
CDCP1 is a transmembrane glycoprotein of molecular weight 140 kDa. CDCP1 is a ligand for a receptor molecule (CD6) that is expressed on some T-cells and is thought to be involved in T-cell migration and chemotaxis. It has a large extracellular domain that includes two CUB domains, and a smaller intracellular domain. The smaller intracellular domain contains five tyrosine residues (Y707, Y734, Y743, Y762 and Y806). CDCP1 is cleaved next to Arg368 (at the extracellular domain) by serine proteases. This cleavage yields a truncated molecule with a molecular weight of 80 kDa.1 CDCP1 is not typically cleaved In vivo. However, its cleavage can be induced during tumorigenesis or tissue injury.2 CDCP1 acts as a substrate for Src family kinases which exclusively mediate the phosphorylation of CDCP1. In cultured cells, tyrosine phosphorylation of CDCP1 transpires when cells are stimulated to detach via trypsin or EDTA. This detachment is associated with the phosphorylation of CDCP1 along with the simultaneous dephosphorylation of focal adhesion proteins. Conversely, during cellular attachment, CDCP1 is dephosphorylated, while focal adhesion proteins are simultaneously phosphorylated. Furthermore, CDCP1 is suspected to play a role in autoimmune diseases such as encephalomyelitis, multiple sclerosis and inflammatory arthritis.3

Antigen Details

PubMed
NCBI Gene Bank ID
Research Area
Cell Biology
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Immunology
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Neuroscience
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Neuroscience Cell Markers
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Stem Cell

References & Citations

1. Moasser, MM. et al. (2005) Oncogene. 24(34):5333-43.
2. Moasser, MM. et al. (2013) Cancer Res. 73(3):1168-79.
3. Lin, F. et al. (2017) Proc Natl Acad Sci U S A. 114(33):E6912-21.
Flow Cytometry
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Formats Available

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Products are for research use only. Not for use in diagnostic or therapeutic procedures.