Anti-Human PD-L1 (CD274) (Clone 29E.2A3) – Purified in vivo PLATINUM™ Functional Grade

Anti-Human PD-L1 (CD274) (Clone 29E.2A3) – Purified in vivo PLATINUM™ Functional Grade

Product No.: P603

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Clone
29E.2A3
Target
PD-L1
Formats AvailableView All
Product Type
Hybridoma Monoclonal Antibody
Alternate Names
CD274, B7-H1, Programmed death-ligand 1
Isotype
Mouse IgG2b κ
Applications
B
,
FA
,
FC
,
IHC

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Select Product Size
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Antibody Details

Product Details

Reactive Species
Human
Host Species
Mouse
Immunogen
Full length Human PD-L1
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
≤ 0.5 EU/mg as determined by the LAL method
Purity
≥95% by SDS Page
≥98% monomer by analytical SEC
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
State of Matter
Liquid
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -70°C. Avoid Repeated Freeze Thaw Cycles.
Regulatory Status
Research Use Only
Country of Origin
USA
Shipping
2 – 8° C Wet Ice
Additional Applications Reported In Literature ?
B,
FA,
IHC,
FC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Human PD-L1 (CD274)
Background
Programmed cell death 1 ligand 1 (PD-L1; CD274; B7-H1) is a type I transmembrane glycoprotein widely expressed in many types of tissues that acts as a ligand for the immune inhibitory receptor programmed cell death 1 (PD-1; CD279)1, 2, 3. The PD-1 pathway is responsible for T cell activation, proliferation, and cytotoxic secretion, with PD-1/PD-L1 interaction triggering inhibitory signals that dampen T cell function. PD-L1 also plays a critical role in the differentiation of inducible regulatory T cells4.

In normal tissues, PD-L1/PD-1 ligation is crucial to maintaining homeostasis of the immune system and preventing autoimmunity during infection and inflammation4. In the tumor microenvironment, their interaction provides an immune escape mechanism for tumor cells by turning off cytotoxic T cells. As such, blocking the PD-L1/PD-1 interaction is a target of many anti-cancer immunotherapies.

29E.2A3 was generated by immunizing female BALB/c mice with purified hPD-L1 cDNA5. Spleen cells were fused with SP2/0 myeloma cells, and the resulting hybridomas were screened by ELISA for reactivity against hPD-L1–Ig fusion protein followed by cell-surface staining of hPD-L1–transfected Chinese hamster ovary cells and 300.19 cells.
Antigen Distribution
PD-L1 is commonly expressed on the surface of antigen presenting cells (macrophages, activated B cells, dendritic cells), some epithelial cells under inflammatory conditions, some activated T cells, and several types of tumors as well as tumor infiltrating immune cells. PD-L1 can also exist in a soluble form (sPD-L1) in myeloid-derived cells (monocytes, macrophages, and dendritic cells) and several human cancer lines.

Antigen Details

Ligand/Receptor
PD-1 (CD279)
NCBI Gene Bank ID
UniProt.org
Q9NZQ7
Research Area
Apoptosis
.
Cancer
.
Cell Biology
.
Cell Death
.
Immunology
.
Inhibitory Molecules
.
Tumor Suppressors

References & Citations

1. Freeman GJ, Long AJ, Iwai Y, et al. J Exp Med. 2000192(7):1027-1034. 2000.
2. Tsai KK, Zarzoso I, Daud AI. Hum Vaccin Immunother. 10(11):3111-3116. 2014.
3. Han Y, Liu D, Li L. Am J Cancer Res. 10(3):727-742. 2020.
4. Dermani FK, Samadi P, Rahmani G, et al. J Cell Physiol. 234(2):1313-1325. 2019.
5. Latchman Y, Wood CR, Chernova T, et al. Nat Immunol. 2(3):261-268. 2001.
6. Brown JA, Dorfman DM, Ma FR, et al. J Immunol. 170(3):1257-1266. 2003.
7. Cai G, Karni A, Oliveira EM, et al. Cell Immunol. 230(2):89-98. 2004.
8. Porichis F, Hart MG, Zupkosky J, et al. J Virol. 88(5):2508-2518. 2014.
9. Hughes MJ, McGettrick HM, Sapey E. J Immunol Methods. 483:112795. 2020.
10. Boyerinas B, Jochems C, Fantini M, et al. Cancer Immunol Res. 3(10):1148-1157. 2015.
11. Nakamoto N, Cho H, Shaked A, et al. PLoS Pathog. 5(2):e1000313. 2009.
12. Hegde S, Lockridge JL, Becker YA, et al. J Autoimmun. 37(1):28-38. 2011.
13. Broos K, Lecocq Q, Keersmaecker B, et al. Vaccines (Basel). 7(3):85. 2019.
14. Darga EP, Dolce EM, Fang F, et al. PLoS One. 16(11):e0260124. 2021.
B
FA
Flow Cytometry
IHC

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.