Anti-Human Retinoblastoma (Rb-1) – Purified

Home/Primary Monoclonal Antibodies/Tumor Markers/Anti-Human Retinoblastoma (Rb-1) – Purified

Pricing & Details

Product No.R125
Clone
Rb1
Protein
Retinoblastoma
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Tumor Suppressor Protein
Isotype
Mouse
IgG1
Applications
IHC FF
,
IP
Prod No.
Size
Price
Avail.
Qty
Add to cart
R125-0.25 mg
0.25 mg
$267.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Mouse
Immunogen
Recombinant human Rb1 protein
Product Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This purified antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Mouse Anti-Human Retinoblastoma (Rb) (Clone Rb1) recognizes an epitope on Human Retinoblastoma (Rb). This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype.
Background
The Rb antigen is a phosphoprotein that is expressed in most normal cells of vertebrates. It is a tumor suppressor gene which functions as a negative regulator of the cell cycle by interacting with transcription factors including E2F1, PU1, ATF2, UBF, Elf1 and cAbl (1, 2). This ability of Rb to alter transcription is regulated by phosphorylation catalyzed by the cyclin dependent protein kinases (cdks) (3, 4). Rb is phosphorylated on serine and threonine, but not on tyrosine residues. The active, underphosphorylated form of Rb (Rb or pRb) is primarily found in resting or fully differentiated cells. The hyperphosphorylated form (ppRb) is primarily found in proliferating cells. The underphosphorylated form of Rb may bind to viral oncogenes such as SV40 large T Ag, adenoviral EIA and HPV-E7, which may contribute to the transforming activity of these viral oncoproteins. Recent evidence shows that the Rb protein is cleaved by caspases. A caspase-resistant Rb can attenuate the death response to tumor necrosis factor–α, suggesting that Rb degradation contributes to the activation of cell-death pathway (5). Rb also stabilizes constitutive heterochromatin to maintain the overall chromatin structure. Aberrations in the Rb gene have been implicated in cancers of breast, colon, prostate, bladder, kidney, nasopharynx and leukemia, as well as the childhood cancer retinoblastoma (RB) (6).

Antigen Details

Research Area
Apoptosis
.
Cell Death
.
Tumor Suppressors

References & Citations

1. Das, S. K. et al. (2005) Biochim. Biophys. Acta 1726:328 2. Wu, C. L. et al. (1995) Mol. Cell. Biol. 15:2536 3. Münger, K. et al. (2002) Virus Res. 89:213 4. Vietri, M. et al. (2006) Cancer Cell Int. 6:3 5. Tan, X. et al. (1997) J. Biol. Chem. 272:9613 6. Murphree, A. L. et al. (1984) Science 223:1028
IHC
Immunoprecipitation Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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