Anti-Mouse CD137L (4-1BBL) – Purified in vivo PLATINUM™ Functional Grade
Anti-Mouse CD137L (4-1BBL) – Purified in vivo PLATINUM™ Functional Grade
Product No.: L320
Clone TKS-1 Target 4-1BBL Formats AvailableView All Product Type Monoclonal Antibody Alternate Names 4-1BBL, CD137L, TNFSF9, 4-1BB Ligand, TKS-1 Isotype Rat IgG2a κ Applications FC , in vivo |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Dilution Buffer Immunogen BALB/c mouse B lymphoma line 2PK-3 Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM<sup>TM</sup> antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C Additional Applications Reported In Literature ? FC Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity TKS-1 activity is directed against 4-1BBL (CD137L). Background 4-1BBL (CD137L) and 4-1BB (CD137) are a receptor ligand pair belonging to the tumor necrosis factor (TNF) receptor/TNF superfamily1,2. 4-1BBL is a type II transmembrane protein expressed by splenic B and T cells, macrophages, and dendritic cells1-5. Members of the TNF-TNF receptor superfamily play critical roles in regulating cellular activation, differentiation, and apoptosis6 and the 4-1BBL-4-1BB interaction is important in cellular immune responses5. 4-1BBL-4-1BB interaction provides a co-stimulatory signal to T cells, resulting in increased proliferation and cytokine production5.
A TKS-1-producing hybridoma was generated by immunizing a SD rat with 4-1BBL-transfected NRK cells (rat IgG2a,κ)5. Splenocytes were fused with P3U1 cells and hypoxanthine–aminopterin–thymidine selection was performed. The monoclonal antibody was identified by its strong reactivity with 4-1BBL-transfected L5178Y cells, cloned by limiting diffusion, purified from ascites, and found to bind to 4-1BBL/NRK and 4-1BBL/P815 cells5. Pre-incubation with TKS-1 blocks 4-1BB–Ig binding to 4-1BBL/L5178Y cells, indicating TKS-1 is specific to mouse 4-1BBL and can interrupt the interaction between 4-1BBL and 4-1BB5. Additionally, TKS-1 can block anti-CD3-stimulated T cell proliferation co-stimulated by 4-1BBL /P815 cells. The monoclonal antibodies 19H3 and TKS-1 bind to different sites on murine 4-1BBL, with TKS-1 binding to a site that overlaps with the receptor binding site7. Additionally, TKS-1 and 4-1BB bind to a similar site, making TKS-1 especially useful for blocking studies. Antigen Distribution 4-1BBL is expressed by activated B cells, macrophages, and dendritic cells. Ligand/Receptor 4-1BB (CDw137) Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. The most common in vivo application of clone TKS-1 in mice is to block or study the function of mouse 4-1BB ligand (4-1BBL, also called CD137L), a critical costimulatory molecule involved in T cell activation and survival, particularly of CD8+ T cells in immune and antitumor responses. Key in vivo applications of TKS-1 in mice include:
TKS-1 is well-validated for in vivo use in blocking studies and mechanistic immunological assays in mice, making it a critical reagent for dissecting the functional role of 4-1BBL in T cell biology and antitumor immunity. Commonly Used Proteins and Antibodies Alongside TKS-1TKS-1 is most commonly referenced as a monoclonal antibody targeting mouse 4-1BBL (CD137L), a member of the tumor necrosis factor receptor superfamily. In the literature, TKS-1 is often used in combination with other antibodies or proteins to investigate immunological mechanisms, particularly in the context of T-cell activation, migration, and antitumor responses. Directly Associated Antibodies and Proteins
Broader Context: Other Antibodies and Targets in Similar ResearchWhile not specifically used with TKS-1 in the provided literature, it is common in immunological research to encounter antibodies against:
Potential Mechanisms and Pathways
Summary Table
Key Points
If you have a specific experimental context or disease model in mind, additional antibodies or proteins may be relevant, but based on the available literature, 3H3 and mgp100 are the most directly associated with TKS-1 in published studies. Key findings from scientific literature citing clone TKS-1 focus on its specificity, functional properties, and applications in immunology research, particularly in the context of mouse 4-1BB ligand (4-1BBL/CD137L) biology.
In summary, literature citations of TKS-1 emphasize its high specificity for mouse 4-1BBL, its capacity to block receptor-ligand interaction, and its mild agonist activity relative to other clones, making it a preferred blocking antibody for mechanistic studies of 4-1BB/4-1BBL signaling in murine immunology. There is currently no published standard dosing regimen specific for clone TKS-1 (anti-mouse CD137L/4-1BBL) across different mouse models. According to product information resources and available literature, dosing protocols for TKS-1 have not been systematically reported or standardized in the scientific literature as of the present. Key context:
Implications:
In summary: References & Citations1. Goodwin RG, Din WS, Davis-Smith T, et al. Eur. J. Immunol. 23:2631-2641. 1993.
2. Alderson MR, Smith CA, Tough TW, et al. Eur. J. Immunol. 24:2219-2227. 1994. 3. Pollok KE, Kim YJ, Hurtado J, et al. Eur J Immunol. 24(2):367-374. 1994. 4. DeBenedette MA, Shahinian A, Mak TW, et al. J. Immunol. 158:551-559. 1997. 5. Futagawa T, Akiba H, Kodama T, et al. Int Immunol. 14(3):275-286. 2002. 6. Smith CA, Farrah T, Goodwin RG. Cell 76:959-962. 1994. 7. Mbanwi AN, Lin GHY, Wang KC, et al. J Immunol Methods. 450:81-89. 2017. 8. Zheng G, Wang B, Chen A. J Immunol. 173(4):2428-2434. 2004. 9. Madireddi S, Eun SY, Lee SW, et al. J Exp Med. 211(7):1433-1448. 2014. 10. Shrestha S, Noh JM, Kim SY, et al. Oncoimmunology. 5(1):e1067744. 2015. 11. Kang SW, Lee SC, Park SH, et al. Cancer Res. 77(21):5989-6000. 2017. Technical ProtocolsCertificate of Analysis |
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