Anti-Mouse CD106 (VCAM-1) (Clone M/K-2.7) – Purified in vivo PLATINUM™ Functional Grade
Anti-Mouse CD106 (VCAM-1) (Clone M/K-2.7) – Purified in vivo PLATINUM™ Functional Grade
Product No.: C2492
Clone M/K-2.7 Target CD106 (VCAM-1) Formats AvailableView All Product Type Hybridoma Monoclonal Antibody Alternate Names VCAM-1, INCAM-110 Isotype Rat IgG1 κ Applications IF , in vivo , N |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Dilution Buffer Immunogen Stromal cells derived from mouse bone marrow Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM<sup>TM</sup> antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Regulatory Status Research Use Only Country of Origin USA Shipping 2 – 8° C Wet Ice Additional Applications Reported In Literature ? IF, in vivo, N Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity The M/K-2.7 activity is specifically directed against mouse CD106 also known as VCAM-1 and
INCAM-110. Background CD106 is a single-chain type I glycoprotein with a molecular weight of 110 kDa. It is upregulated in
response to inflammatory stimuli and cytokines, and it plays an important role in leukocyte adhesion,
transmigration, and T-cell proliferation by binding to integrins CD49d/CD29 (VLA-4) and α4β71. It has
implications in several pathologies, including heart diseases, inflammation, and cancer metastasis2. The
regulation and function of CD106 in immune responses highlight its potential as a therapeutic target in
treating these conditions. The M/K-2.7 clone was developed using stromal cells derived from mouse bone marrow as the immunogen. It has been widely used in various research contexts, particularly in studies involving in vivo VCAM-1 neutralization, immunofluorescence techniques, and more. This clone demonstrates its versatility across a range of experimental setups and is a valuable tool for investigating vascular cell adhesion mechanisms and the inflammatory process. It is particularly useful for research focused on inflammatory processes, immune cell migration, and the study of vascular biology3-6. Antigen Distribution CD106 is predominantly expressed on activated vascular endothelial cells, as well
as on various other cells including follicular and interfollicular dendritic cells, some macrophages, and
bone marrow stromal cells. Its expression can also be found in non-vascular cells within joints, kidneys,
muscles, the heart, the placenta, and the brain. Ligand/Receptor VLA-4 (α4/β1 integrin) and LPAM-1 (α4/β7 integrin) NCBI Gene Bank ID UniProt.org Research Area Cell Adhesion . Cell Biology . Immunology . Neuroinflammation . Neuroscience . CD Molecules . Stem Cells Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Applications of M/K-2.7 Clone in In Vivo Mouse StudiesThe M/K-2.7 monoclonal antibody targets mouse CD106 (VCAM-1), a cell adhesion molecule highly expressed on activated vascular endothelial cells and involved in leukocyte recruitment during inflammation. Its use in in vivo mouse studies spans several key research areas: VCAM-1 Neutralization and Functional Studies Vascular Biology and Inflammation Research Drug Delivery and Targeting Typical Experimental Use Summary Table: In Vivo Uses of M/K-2.7
ConclusionThe M/K-2.7 clone is a versatile tool in mouse in vivo research, primarily used to neutralize VCAM-1 for functional studies, investigate inflammatory and vascular biology, and enhance targeted drug delivery to sites of VCAM-1 expression. Its specificity and efficacy in vivo have made it a staple in both basic and translational immunology research. The correct storage temperature for the sterile packaged clone M/K-2.7 is 4°C for up to 12 months, or –20°C for long-term storage. It is critical to avoid freeze-thaw cycles, as these can compromise the stability and activity of the antibody. These recommendations are standard for many monoclonal antibodies and are consistent with broader guidelines for antibody storage, which suggest aliquoting to minimize repeated thawing and emphasize storing antibodies away from light and in a stable, temperature-controlled environment. If you plan to store the antibody for longer than a year, transferring it to –20°C is advisable, and for very long-term storage (multiple years), –80°C is generally preferred for most antibodies, though the specific product datasheet should always be consulted for clone-specific instructions. Commonly Used Antibodies and Proteins with M/K-2.7 in the LiteratureM/K-2.7 is a monoclonal antibody specific for mouse CD106 (VCAM-1), widely used in research on inflammation, immune cell migration, and vascular biology. In experimental contexts, M/K-2.7 is often employed alongside other antibodies and proteins to study complex biological processes, particularly those involving leukocyte-endothelial interactions and immune responses. Key Antibodies and Proteins Used with M/K-2.7
Example Research Contexts
Summary Table: Common Pairings with M/K-2.7
ConclusionM/K-2.7 is most frequently paired with antibodies against its integrin ligands (VLA-4, ?4?7), markers for endothelial and leukocyte identification, and inflammatory cytokines that modulate VCAM-1 expression. These combinations enable detailed investigation of VCAM-1s role in immune cell trafficking, inflammation, and vascular biology. Clone M/K-2.7 is a monoclonal antibody targeting mouse CD106 (VCAM-1) and is widely cited in the literature for its ability to specifically bind and functionally neutralize VCAM-1, a vascular adhesion molecule implicated in leukocyte adhesion, migration, and inflammatory processes. Key findings from citations involving M/K-2.7 include:
In summary, clone M/K-2.7 is a benchmark antibody for studying VCAM-1 function in murine models, and its neutralizing ability is crucial for dissecting mechanisms in inflammation, immunity, and vascular biology research. References & Citations1. Tolstrup A, Hokland P, Nielsen B, Justesen J, Hokland M. J Interferon Res. 1993;13(6):433-441. 2. Salajegheh A, Salajegheh A. Springer International Publishing; 2016:375-379. 3. Hession C, Moy P, Tizard R, et al. Biochem Biophys Res Commun. 1992;183(1):163-169. 4. Osborn L, Hession C, Tizard R, et al. Cell. 1989;59(6):1203-1211. 5. Miyake K, Medina K, Ishihara K, Kimoto M, Auerbach R, Kincade PW. The Journal of cell biology. 1991;114(3):557-565. 6. Kumar AG, Dai XY, Kozak CA, Mims MP, Gotto AM, Ballantyne CM. The Journal of Immunology. 1994;153(9):4088-4098. 7. Yousef H, Czupalla CJ, Lee D, et al. Nat Med. 2019;25(6):988-1000. 8. de Juan A, Ince LM, Pick R, et al. Circulation. 2019;140(13):1100-1114. 9. He W, Holtkamp S, Hergenhan SM, et al. Immunity. 2018;49(6):1175-1190.e7. 10. Kapitsinou PP, Sano H, Michael M, et al. J Clin Invest. 2014;124(6):2396-2409. 11. Chow A, Huggins M, Ahmed J, et al. Nat Med. 2013;19(4):429-436. 12. Brinkman CC, Rouhani SJ, Srinivasan N, Engelhard VH. J Immunol. 2013;191(5):2412-2425. 13. Thomas SY, Scanlon ST, Griewank KG, et al. J Exp Med. 2011;208(6):1179-1188. |
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Products are for research use only. Not for use in diagnostic or therapeutic procedures.
