Anti-Mouse CD178 (FasL) – APC

Anti-Mouse CD178 (FasL) – APC

Product No.: M355

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Clone
MFL3
Target
CD178
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
FasL, Fas Ligand, FasL, Apo-1 Ligand, CD95 Ligand, TNFSF6
Isotype
IgG
Applications
FC

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Armenian Hamster
Immunogen
B6 mouse FasL cDNA-transfected baby hamster kidney (B6 FasL/BHK) cells
Product Concentration
0.2 mg/ml
Formulation
This Allophycocyanin (APC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
This Allophycocyanin (APC) conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Excitation Laser
Red Laser (650 nm)
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this CD178 antibody, clone MFL3, for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl. Titration of the reagent is recommended for optimal performance for each application.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone MFL3 recognizes an epitope on mouse FasL.
Background
FasL antibody, clone AFS98, recognizes Fas ligand (FasL), also known as CD178, Apo-1 ligand, and CD95 ligand. FasL is a 40 kDa type II integral membrane protein that belongs to the tumor necrosis factor (TNF) superfamily. FasL is expressed by activated T cells and natural killer (NK cells)1-3. Binding of FasL to its receptor Fas (CD95, APO-1) induces apoptotic cell death in Fas-expressing target cells, contributing to anti-viral immunity. FasL also contributes to peripheral tolerance and the downregulation of immune responses through activation-induced autocrine and paracrine T cell death4. FasL is also found in the anterior chamber of the eye and on Sertoli cells in the testis, and is implicated in immune-privilege at these sites5,6. FasL also contributes to CD8 proliferation and neutrophil recruitment7,8. Soluble FasL (26 kDa) can be released following cleavage by metalloproteinases and block FasL-mediated signaling9. Fas/FasL-signaling is involved in the development of many human diseases, including autoimmunity and cancer10. Many human tumors over-express FasL, resulting in tumor infiltrating lymphocyte (TIL) apoptosis and immune evasion, which is associated with poor prognosis11-14.
Antigen Distribution
FasL is expressed on activated T cells, NK cells, the eye, and testis.

Antigen Details

Ligand/Receptor
Fas (CD95)
PubMed
NCBI Gene Bank ID
Research Area
Apoptosis
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Cell Biology
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Cell Death
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Immunology
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Neuroscience
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Tumor Suppressors

References & Citations

1. Okumura K., et al. (1994) Proc Natl Acad Sci USA. 91:4930–4934
2. Nagata S., et al. (1995) J Immunol. 154:3806–3813
3. Saito T., et al. (1995) J Exp Med. 181:1235–1238
4. Ferguson T A., et al. (1995) Science. 270:1189–1192
5. Duke R C., et al. (1995) Nature (London). 377:630–632
6. Fink PJ. (2000) J Immunol. 165(10):5537-43
7. Matsuzawa A., et al. (1998) J Immunol. 161: 4484–4488
8. Nagata S., et al. (1998) Nat Med. 4(1):31-6
9. Hueber AO., et al (2019) Cancers (Basel). 11(5):639
10. Kabelitz D., et al. (2000) Cancer Res. 60: 822–828
11. Giannarelli D., et al. (2000) Int J Cancer. 89: 127–132
12. Kanno H., et al. (2000) Br J Cancer. 82: 1446–1452.
13. Nagano H., et al. (Cancer) Br J Cancer. 82: 1211–1217
Flow Cytometry

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.