Anti-Mouse CD178 (FasL) – Purified in vivo GOLD™ Functional Grade

Applications of Clone MFL3 in In Vivo Mouse Studies

Clone MFL3 is a hamster-derived monoclonal antibody that specifically targets mouse Fas Ligand (FasL, CD178, CD95 Ligand, TNFSF6), a type II transmembrane glycoprotein in the TNF superfamily. It is widely used in immunological research to study the biological roles of FasL in mice, including immune regulation, apoptosis, and immune evasion by tumors.

Key Uses of MFL3 in Mouse Models

• Blocking Apoptotic Signaling:
  MFL3 has been reported to efficiently block the cytotoxicity mediated by mouse Fas Ligand, thereby inhibiting CD178/CD95-induced apoptosis in vivo. This is particularly useful for investigating the role of the Fas/FasL pathway in immune homeostasis, autoimmunity, and inflammation.
• Modulating Immune Responses:
  The Fas/FasL interaction is essential for peripheral tolerance and the regulation of immune responses, including T-cell development and the control of activated lymphocytes. MFL3 can be used to manipulate these pathways, such as by preventing the elimination of autoreactive lymphocytes or by altering T-cell-mediated cytotoxicity.
• Tumor Immunology:
  Some tumors upregulate FasL to induce apoptosis of infiltrating immune cells, thus evading immune surveillance. MFL3 can be used to block this tumor-protective mechanism, potentially enhancing anti-tumor immune responses in mouse models of cancer.
• Inflammation and Leukocyte Trafficking:
  Beyond apoptosis, FasL is also involved in neutrophil extravasation and chemotaxis. MFL3 can be used to study the inflammatory roles of FasL in vivo, for example, in models of inflammation or tissue injury.
• Functional Grade for In Vivo Use:
  MFL3 is available in functional grade (low endotoxin, high purity), specifically formulated for in vivo administration in mice, allowing for direct manipulation of the Fas/FasL pathway in live animals.

Technical Considerations

• Specificity: MFL3 recognizes an epitope on mouse FasL and does not cross-react with rat leukocytes, making it a reliable tool for mouse studies.
• Activation Dependence: FasL is expressed at low levels on resting cells but is upregulated on activated T cells. To detect FasL expression ex vivo, cells must be appropriately activated, for example, with anti-CD3 antibodies.
• Formulation: The antibody is typically administered intraperitoneally or intravenously, with dosing optimized for the experimental model and desired biological effect. Since applications vary, each investigator should titrate the reagent to achieve optimal results.
• Control of Autoimmunity: Mice with defective FasL (gld mutants) develop autoimmune lymphoproliferative disease; MFL3 can be used to phenocopy or rescue aspects of this phenotype by modulating FasL activity.

Summary Table: In Vivo Uses of Clone MFL3

Conclusion

Clone MFL3 is a versatile tool for in vivo mouse studies, enabling researchers to block FasL-mediated apoptosis, modulate immune responses, investigate tumor immune evasion, and study inflammation. Its specific reactivity, functional blocking capability, and availability in in vivo-grade formulations make it a valuable reagent for dissecting the complex roles of the Fas/FasL pathway in mouse models.

I cannot find specific storage temperature information for the sterile packaged clone MFL3 in the provided search results. The search results contain storage temperature guidelines for various laboratory materials including Gibson Assembly kits, lambda vectors, ELISA kits, and general sterile supplies, but none specifically mention MFL3.

General Guidelines for Sterile Packaged Materials

While I cannot provide the exact storage temperature for MFL3, the search results do offer some general principles for storing sterile packaged items:

Standard Storage Conditions: The storage area for packaged sterilized items should be well ventilated and protected against dust, moisture, insects, and temperature and humidity extremes.

Common Laboratory Storage Temperatures: Many sterile biological materials are typically stored at standard laboratory temperatures such as:

• -80°C for long-term storage of sensitive biological materials
• -20°C for intermediate-term storage
• 4°C for short-term storage (up to one month for some kits)

Recommendation

To determine the correct storage temperature for your specific sterile packaged clone MFL3, I recommend:

1. Check the product documentation or packaging insert that came with MFL3
2. Contact the manufacturer directly for official storage guidelines
3. Consult your institution's protocols for similar sterile cloned materials

Without access to the specific product information for MFL3, I cannot provide the definitive storage temperature, as different cloned materials may have varying storage requirements depending on their composition and intended use.

Commonly Used Antibodies and Proteins with MFL3 in the Literature

MFL3 is a well-known Armenian hamster monoclonal antibody targeting mouse Fas ligand (CD178). It is widely used in immunological research, particularly in studies investigating apoptosis, T cell activation, and immune regulation.

Directly Co-Used Antibodies and Proteins

• Anti-CD3 Antibodies: In immunological research, especially studies focused on T cell activation and apoptosis, MFL3 (anti-FasL) is often used alongside anti-CD3 antibodies to activate T cells and then assess FasL expression or function on activated T cells. While the search results do not specify a particular study pairing MFL3 with anti-CD3, this combination is frequently reported in the broader immunological literature, as anti-CD3 is a standard stimulus for T cell activation, and FasL (CD178) is upregulated upon T cell activation.
• Anti-Fas (CD95) Antibodies: Fas ligand (FasL, CD178) exerts its apoptotic effect by binding to the Fas receptor (CD95). Therefore, antibodies against Fas (CD95) are sometimes used in parallel with MFL3 to study the Fas–FasL apoptotic pathway.
• Conjugation Partners and Controls: MFL3 is available in a phycoerythrin (PE)-conjugated form, and in flow cytometry, it is routinely paired with other fluorescently labeled antibodies (e.g., anti-CD4, anti-CD8, anti-CD25) to enable multiparametric analysis of immune cell populations. However, the literature does not specify a standard or exclusive "partner" antibody; the combination depends on the experimental design.

Other Contextual Uses in the Literature

While the provided search results do not detail specific co-administration of MFL3 with Fc fusion proteins or albumin fusions, these protein classes are commonly used in antibody and protein therapeutic research, including studies on half-life extension and targeted delivery. However, there is no direct evidence that MFL3 is routinely used with these molecules in the cited literature.

Summary Table

Conclusions

MFL3 is most commonly used in the literature alongside anti-CD3 antibodies and anti-Fas (CD95) antibodies for investigating T cell activation and apoptosis pathways, respectively. In flow cytometry, it is paired with a range of fluorescently labeled antibodies for detailed immune cell analysis. No standard co-use with Fc fusion proteins or albumin fusions is indicated in the provided literature.

Clone MFL3 is a monoclonal antibody widely cited in scientific literature for its role in detecting and functionally blocking mouse Fas Ligand (FasL, CD178), a key mediator in apoptotic cell death and immune regulation.

Key findings from citations of clone MFL3 in scientific literature include:

• Specificity to Mouse FasL (CD178): MFL3 reacts with mouse FasL, a member of the TNF superfamily expressed on activated T cells, NK cells, and immune-privileged sites like the eye and testis.
• Functional Blocking of Apoptosis: MFL3 has been reported to block FasL-induced apoptosis, demonstrating utility in functional studies of T cell regulation and cell-mediated cytotoxicity.
• Immunological Applications: The antibody is primarily used for flow cytometry (FCM), allowing quantification and characterization of FasL-expressing cells in murine models. Recommended concentration for flow cytometry is ? 0.25 ?g per 10^6 cells in 100 ?L volume.
• Contribution to Peripheral Tolerance and Anti-Viral Immunity: Research using MFL3 shows FasL’s critical role in inducing apoptosis of activated T cells, contributing to the maintenance of immune tolerance and regulation of immune responses.
• Citations and Usage: Product databases and catalogs indicate clone MFL3 is cited in at least 5 scientific publications for its role in murine immunology and apoptosis research.

Summary of supporting details:

• Background: FasL (CD178) is a 40 kDa transmembrane protein involved in triggering apoptosis through interaction with its receptor Fas (CD95).
• Mechanistic Insight: Studies using MFL3 have helped elucidate the role of FasL in immune privileged sites and in T cell developmental processes.
• Experimental Utility: Clone MFL3's blocking activity is utilized to experimentally dissect FasL-mediated apoptotic pathways and their implications in immune response and disease models.

In sum, clone MFL3 citations underscore its importance as an investigative tool in murine FasL biology, immune regulation, and functional blocking of apoptosis in preclinical studies.