Anti-Mouse CD226 (DNAM-1) [Clone 480.1] — Purified in vivo GOLD™ Functional Grade
Anti-Mouse CD226 (DNAM-1) [Clone 480.1] — Purified in vivo GOLD™ Functional Grade
Product No.: D391
Clone 480.1 Target DNAM-1 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names DNAX Accessory Molecule 1, CD226, DNAM1, PTA1, TLiSA1 Isotype Rat IgG2a κ Applications FC , in vivo |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Dilution Buffer Immunogen Th1 polarized T cell clones Product Concentration ≥ 5.0 mg/ml Endotoxin Level <1.0 EU/µg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2829933 Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 480.1 activity is directed against murine CD226 (DNAM-1; PTA-1). Background Murine (m) CD226 (DNAM-1; PTA1) is an adhesion molecule involved in intercellular adhesion, lymphocyte signalling, cytotoxicity, and lymphokine secretion mediated by cytotoxic T-lymphocyte and NK cells (Uniprot Q8K4F0)1. mCD226 is also essential for NK-mediated killing of immature dendritic cells2 and acts as an important costimulatory molecule in Th1 cell activation and effector function3.
CD226 binds to PVR (CD155) via its N-terminal Ig-like domain2 and with cell surface receptor NECTIN2 (CD112)1. Based on its similarity to human CD226 (Uniprot Q15762), mCD226 likely competes with PVRIG for NECTIN2 binding. mCD226 is specifically expressed on the surface of differentiated Th1 cells but not Th2 or Th0 cells3. mCD226 expression is enhanced by Th1 differentiation but down-regulated upon Th2 polarization. In vivo treatment with anti-CD226 (clone 10E5) inhibits T cell activation and delays the onset and reduces the severity of Th1-mediated autoimmune disease. Additionally, mCD226 is constitutively expressed on CD8+ T cells, subsets of naïve CD11b+ macrophages and NK cells, and is up-regulated on activated CD4+ cells2,3. mCD226 is a marker for mature T cells2. Antigen Distribution mCD226 is expressed on the surface of differentiated Th1 cells, CD8+ T cells, subsets of naïve CD11b+ macrophages and NK cells, and activated CD4+ cells. PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. There is no direct information in the provided search results about a specific "clone 480.1" used in in vivo mouse studies. None of the results mention this clone number or describe its experimental use. However, the search results do describe the use of other specific clones in mouse in vivo researchfor example, PD-1 antibody clones such as RMP1-14, J43, and 29F.1A12, which are routinely used to block the PD-1 pathway and study immune responses in cancer and infection models. These clones are selected based on their binding properties, efficacy in various disease models, and compatibility with therapeutic studies. If "480.1" refers to a similar antibody clone, it likely would be used to target a specific protein or cell surface marker in vivo, but without more context or direct evidence, its exact purpose and experimental application in mouse studies cannot be determined from the current search results. If you have additional details about the origin or specificity of clone 480.1 (e.g., target antigen, species, or research field), please provide them for a more precise answer. Otherwise, based on the available information, clone 480.1's use in in vivo mouse studies is not documented here. There were no search results that specifically mention a sterile packaged clone "480.1." Therefore, I can't provide a direct answer regarding the exact storage temperature for this product. If "clone 480.1" refers to a biological reagent or cell culture product, general best practices for such materials usually involve freezing at -20°C or lower for long-term storage, or refrigeration at 2–8°C for short-term or ready-to-use aliquots. However, without manufacturer-specific documentation, it is essential to consult the product datasheet or directly contact the supplier for precise storage instructionsimproper storage can compromise product integrity and experimental results. If your query refers to a different type of product (e.g., vaccine, antibody), please clarify, and I can provide more targeted guidance based on available data. The query does not specify what "480.1" refers to in the context of antibodies or proteins. However, I can provide some information on commonly used antibodies and proteins in various therapeutic and research contexts based on the available literature:
If "480.1" refers to a specific antibody or protein, more context would be needed to provide a relevant list. I don't have specific information about clone 480.1 citations in the provided search results. The search results contain information about DNAM-1 research involving targeted clones and various scientific studies, but none specifically mention "clone 480.1" or provide details about its key findings in scientific literature. The search results primarily discuss DNAM-1 knockout mice research, bioaccumulation mechanisms, chemical synthesis studies, and Medicare coverage policies, but don't contain the specific information you're looking for regarding clone 480.1 citations. To get accurate information about clone 480.1 and its key findings, you would need to search scientific databases specifically for publications that cite or discuss this particular clone, as the current search results don't provide this information. References & Citations1. https://www.uniprot.org/uniprotkb/Q8K4F0/entry
2. Seth S, Georgoudaki AM, Chambers BJ, et al. J Leukoc Biol. 86(1):91-101. 2009. 3. Dardalhon V, Schubart AS, et al. J Immunol. 175(3):1558-1565. 2005. 4. He Y, Peng H, Sun R, et al. J Autoimmun. 81:1-12. 2017. Technical Protocols  Certificate of Analysis |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
