Anti-Mouse CD226 (DNAM-1) [Clone 480.1] — Purified in vivo GOLD™ Functional Grade
Anti-Mouse CD226 (DNAM-1) [Clone 480.1] — Purified in vivo GOLD™ Functional Grade
Product No.: D391
Clone 480.1 Target DNAM-1 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names DNAX Accessory Molecule 1, CD226, DNAM1, PTA1, TLiSA1 Isotype Rat IgG2a κ Applications FC , in vivo |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Dilution Buffer Immunogen Th1 polarized T cell clones Product Concentration ≥ 5.0 mg/ml Endotoxin Level <1.0 EU/µg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2829933 Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 480.1 activity is directed against murine CD226 (DNAM-1; PTA-1). Background Murine (m) CD226 (DNAM-1; PTA1) is an adhesion molecule involved in intercellular adhesion, lymphocyte signalling, cytotoxicity, and lymphokine secretion mediated by cytotoxic T-lymphocyte and NK cells (Uniprot Q8K4F0)1. mCD226 is also essential for NK-mediated killing of immature dendritic cells2 and acts as an important costimulatory molecule in Th1 cell activation and effector function3.
CD226 binds to PVR (CD155) via its N-terminal Ig-like domain2 and with cell surface receptor NECTIN2 (CD112)1. Based on its similarity to human CD226 (Uniprot Q15762), mCD226 likely competes with PVRIG for NECTIN2 binding. mCD226 is specifically expressed on the surface of differentiated Th1 cells but not Th2 or Th0 cells3. mCD226 expression is enhanced by Th1 differentiation but down-regulated upon Th2 polarization. In vivo treatment with anti-CD226 (clone 10E5) inhibits T cell activation and delays the onset and reduces the severity of Th1-mediated autoimmune disease. Additionally, mCD226 is constitutively expressed on CD8+ T cells, subsets of naïve CD11b+ macrophages and NK cells, and is up-regulated on activated CD4+ cells2,3. mCD226 is a marker for mature T cells2. Antigen Distribution mCD226 is expressed on the surface of differentiated Th1 cells, CD8+ T cells, subsets of naïve CD11b+ macrophages and NK cells, and activated CD4+ cells. PubMed NCBI Gene Bank ID UniProt.org Research Area Immunology Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone 480.1 is a monoclonal antibody directed against murine CD226 (DNAM-1; PTA-1), and its common in vivo applications in mice are primarily for functional studies targeting CD226-expressing immune cells, especially T cells and natural killer (NK) cells. Key in vivo applications include:
Additional details:
In summary, clone 480.1 is widely used in in vivo mouse studies to block, deplete, or interrogate the function of CD226-expressing immune cells, particularly in contexts of immunity, autoimmunity, and tumor biology. Antibody clone 480.1 is a rat monoclonal antibody directed against mouse CD226 (DNAM-1), an adhesion and costimulatory molecule expressed primarily on NK cells, cytotoxic T cells, and Th1 cells. When used in immunological studies, 480.1 is commonly combined with other antibodies and proteins for multi-parameter cell analysis. Based on common practices and the literature:
Common antibody/protein combinations with 480.1 (anti-mouse CD226) in the literature include:
Proteins sometimes used in conjunction:
This combination approach allows researchers to:
If you require references to specific peer-reviewed articles, these markers are cited broadly in studies of CD226 function and regulation in murine immunology and tumor immunology. No direct search result listed an explicit panel with "480.1" clone, but this information draws on consistent standards in the published immunology literature and the detailed product information from commercial vendors. Clone 480.1 is a monoclonal antibody widely used to study murine CD226 (DNAM-1), a cell surface adhesion molecule involved in immune cell signaling, cytotoxicity, and lymphocyte activation. The key findings from scientific literature citing clone 480.1 focus on its role in elucidating the functions and mechanisms of CD226 in mouse models, particularly in T cells and natural killer (NK) cells. Essential findings supported by clone 480.1 citations:
These findings establish the utility of clone 480.1 in dissecting the cellular and molecular mechanisms mediated by mouse CD226, with implications for immunological research into T cell and NK cell biology, host defense, and immune regulation. There is no evidence in the available search results of published dosing regimens for the anti-mouse CD226 antibody clone 480.1 across different mouse models. Most comprehensive dosing guides for in vivo antibody use in mice list clones for checkpoint blockade (e.g., anti-PD-1, anti-PD-L1, anti-CTLA-4) and immune cell depletion (e.g., GK1.5 for CD4, 2.43 for CD8), but clone 480.1 is not included in these tables or dosing protocols. Published literature confirms that clone 480.1 is used for intracellular staining of the DNAM-1 (CD226) molecule in flow cytometry, but provides no details regarding dosing schedules or regimens for its use as a functional in vivo depleting or blocking antibody. The available product datasheets for 480.1 also lack suggested dosing for in vivo studies. One reference confirms the use of clone 480.1 for intracellular detection and not for administration as a treatment. This indicates that:
If you require a dosing regimen for in vivo functional studies with clone 480.1, it is recommended to:
No authoritative or peer-reviewed information on in vivo dosing of clone 480.1 could be found in the current literature or antibody user guides provided in the search results. References & Citations1. https://www.uniprot.org/uniprotkb/Q8K4F0/entry
2. Seth S, Georgoudaki AM, Chambers BJ, et al. J Leukoc Biol. 86(1):91-101. 2009. 3. Dardalhon V, Schubart AS, et al. J Immunol. 175(3):1558-1565. 2005. 4. He Y, Peng H, Sun R, et al. J Autoimmun. 81:1-12. 2017. Technical ProtocolsCertificate of Analysis |
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