Anti-Mouse CD226 (DNAM-1) – PE

Anti-Mouse CD226 (DNAM-1) – PE

Product No.: D393

[product_table name="All Top" skus="D353"]

- -
- -
Clone
480.1
Target
DNAM-1
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
DNAX Accessory Molecule 1, CD226, DNAM1, PTA1, TLiSA1, LFA-1 associated Molecule PTA-1
Isotype
IgG2a κ
Applications
FC

- -
- -
Product Size
- -
- -

Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
Jurkat cells transfected with mouse CD226
Product Concentration
0.2 mg/ml
Formulation
This R-phycoerythrin (R-PE) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
This R-phycoerythrin (R-PE) conjugate is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Excitation Laser
Blue Laser (488 nm) and/or Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Additional Applications Reported In Literature ?
FC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 480.1 activity is directed against murine CD226 (DNAM-1; PTA-1).
Antigen Distribution
mCD226 is expressed on the surface of differentiated Th1 cells, CD8+ T cells, subsets of naïve CD11b+ macrophages and NK cells, and activated CD4+ cells.
Background
Murine (m) CD226 (DNAM-1; PTA1) is an adhesion molecule involved in intercellular adhesion, lymphocyte signalling, cytotoxicity, and lymphokine secretion mediated by cytotoxic T-lymphocyte and NK cells (Uniprot Q8K4F0)1. mCD226 is also essential for NK-mediated killing of immature dendritic cells2 and acts as an important costimulatory molecule in Th1 cell activation and effector function3.

CD226 binds to PVR (CD155) via its N-terminal Ig-like domain2 and with cell surface receptor NECTIN2 (CD112)1. Based on its similarity to human CD226 (Uniprot Q15762), mCD226 likely competes with PVRIG for NECTIN2 binding.

mCD226 is specifically expressed on the surface of differentiated Th1 cells but not Th2 or Th0 cells3. mCD226 expression is enhanced by Th1 differentiation but down-regulated upon Th2 polarization. In vivo treatment with anti-CD226 (clone 10E5) inhibits T cell activation and delays the onset and reduces the severity of Th1-mediated autoimmune disease. Additionally, mCD226 is constitutively expressed on CD8+ T cells, subsets of naïve CD11b+ macrophages and NK cells, and is up-regulated on activated CD4+ cells2,3. mCD226 is a marker for mature T cells2.

Antigen Details

Ligand/Receptor
CD112 and CD155
PubMed
NCBI Gene Bank ID
Research Area
Immunology

References & Citations

1. https://www.uniprot.org/uniprotkb/Q8K4F0/entry
2. Seth S, Georgoudaki AM, Chambers BJ, et al. J Leukoc Biol. 86(1):91-101. 2009.
3. Dardalhon V, Schubart AS, et al. J Immunol. 175(3):1558-1565. 2005.
4. He Y, Peng H, Sun R, et al. J Autoimmun. 81:1-12. 2017.
Flow Cytometry
- -
- -

Formats Available

- -
- -
Products are for research use only. Not for use in diagnostic or therapeutic procedures.