Anti-Mouse CD226 (DNAM-1) – Purified in vivo PLATINUM™ Functional Grade

Home/Bulk In vivo Functional Grade Antibodies, Platinum Purified Bulk Antibodies, Primary Monoclonal Antibodies/Anti-Mouse CD226 (DNAM-1) – Purified in vivo PLATINUM™ Functional Grade

Pricing & Details

Product No.D392
Clone
480.1
Protein
DNAM-1
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
DNAX Accessory Molecule 1, CD226, DNAM1, PTA1, TLiSA1, LFA-1 associated Molecule PTA-1
Isotype
Rat
IgG2a κ
Applications
FC
Prod No.
Size
Price
Avail.
Qty
Add to cart
D392-1.0mg
1.0 mg
$225.00
In stock
Max:
Min: 1
Step: 1
D392-5.0mg
5.0 mg
$445.00
In stock
Max:
Min: 1
Step: 1
D392-25mg
25 mg
$1,285.00
In stock
Max:
Min: 1
Step: 1
D392-50mg
50 mg
$2,045.00
In stock
Max:
Min: 1
Step: 1
D392-100mg
100 mg
$2,800.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Th1 polarized T cell clones
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Other Applications Reported In Literature ?
FC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 480.1 activity is directed against murine CD226 (DNAM-1; PTA-1).
Antigen Distribution
mCD226 is expressed on the surface of differentiated Th1 cells, CD8+ T cells, subsets of naïve CD11b+ macrophages and NK cells, and activated CD4+ cells.
Background
Murine (m) CD226 (DNAM-1; PTA1) is an adhesion molecule involved in intercellular adhesion, lymphocyte signalling, cytotoxicity, and lymphokine secretion mediated by cytotoxic T-lymphocyte and NK cells (Uniprot Q8K4F0)1. mCD226 is also essential for NK-mediated killing of immature dendritic cells2 and acts as an important costimulatory molecule in Th1 cell activation and effector function3.

CD226 binds to PVR (CD155) via its N-terminal Ig-like domain2 and with cell surface receptor NECTIN2 (CD112)1. Based on its similarity to human CD226 (Uniprot Q15762), mCD226 likely competes with PVRIG for NECTIN2 binding.

mCD226 is specifically expressed on the surface of differentiated Th1 cells but not Th2 or Th0 cells3. mCD226 expression is enhanced by Th1 differentiation but down-regulated upon Th2 polarization. In vivo treatment with anti-CD226 (clone 10E5) inhibits T cell activation and delays the onset and reduces the severity of Th1-mediated autoimmune disease. Additionally, mCD226 is constitutively expressed on CD8+ T cells, subsets of naïve CD11b+ macrophages and NK cells, and is up-regulated on activated CD4+ cells2,3. mCD226 is a marker for mature T cells2.

Antigen Details

Protein
PubMed
NCBI Gene Bank ID
Research Area
Immunology

References & Citations

1. https://www.uniprot.org/uniprotkb/Q8K4F0/entry
2. Seth S, Georgoudaki AM, Chambers BJ, et al. J Leukoc Biol. 86(1):91-101. 2009.
3. Dardalhon V, Schubart AS, et al. J Immunol. 175(3):1558-1565. 2005.
4. He Y, Peng H, Sun R, et al. J Autoimmun. 81:1-12. 2017.
Flow Cytometry

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.