Anti-Mouse CD276 (Clone MJ18) – Purified in vivo PLATINUM™ Functional Grade

Pricing & Details

Product No.C3344
Clone
MJ18
Protein
CD276
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
CD276; CD276 antigen; B7h3; B7RP-2; AU016588; 6030411F23Rik
Isotype
Rat
IgG1 κ
Applications
B
,
FC
Prod No.
Size
Price
Avail.
Qty
Add to cart
C3344-1.0mg
1.0 mg
$225.00
In stock
Max:
Min: 1
Step: 1
C3344-5.0mg
5.0 mg
$445.00
In stock
Max:
Min: 1
Step: 1
C3344-25mg
25 mg
$1,285.00
In stock
Max:
Min: 1
Step: 1
C3344-50mg
50 mg
$2,045.00
In stock
Max:
Min: 1
Step: 1
C3344-100mg
100 mg
$2,800.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Immunogen
Mouse B7-H3 IgG2a fusion protein
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUMTM antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at -80°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC
Other Applications Reported In Literature ?
B
FC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
MJ18 activity is directed against mouse CD276 also known as B7-H3.
Antigen Distribution
CD276 is weakly expressed on activated lymphocytes, macrophages, dendritic cells, nasal and airway epithelial cells, osteoblasts, and some tumor cell lines. A soluble form is secreted by monocytes, dendritic cells, and activated T cells.
Background
CD276, also known as B7 homolog 3 protein (B7-H3), is a member of the B7 superfamily and acts as an immune checkpoint molecule and a costimulatory/coinhibitory immunoregulatory protein1. CD276 influences innate and adaptive immunity, regulates the aggressiveness of cancer cells, and is thought to play an important role in tumor development and cancer immunity. CD276 has been studied in many cancers, including breast, lung, ovarian, brain, gastric, and squamous cell carcinoma.

Human CD276 exists as either a soluble isoform or as a ~45–66 kDa type I transmembrane protein that is composed of an extracellular domain, a transmembrane domain, and a short intracellular domain1. In murine CD276, the extracellular domain is composed of a single pair of immunoglobulin variable and constant domains. Soluble CD276 is produced by cleavage from the cell surface or via alternative intron splicing and has been found in the secretomes of exosomes and other extracellular vesicles.

In normal human tissues, CD276 mRNA is widely and abundantly expressed but protein abundance is low1. miR-124 is thought to cause translational repression of CD276 by targeting its 3’-UTR, while other miRNAs are known to affect CD276 expression. In contrast, in tumor cells, CD276 mRNA and protein are abundant, and its presence is correlated with worsened prognosis, poor survival, recurrence rate, and enhanced invasive and migratory properties. CD276 blocking with monoclonal antibodies has been shown to reduce tumor growth and prolong survival in mouse models of various cancers. TREM-like transcript 2 (TLT-2) has been identified as a potential receptor2.

MJ18 was generated by immunizing Sprague Dawley rats with aa 1–242 of mouse CD276 extracellular domain linked to the Fc portion of mouse IgG2a3. After immunization, lymph node cells were fused with P3U1 myeloma cells, hybridomas were selected by flow cytometry, and MJ18 was purified from ascites.

Antigen Details

Protein
PubMed
NCBI Gene Bank ID
UniProt.org
Research Area
Immunology

References & Citations

1. Zhou WT, Jin WL. Front Immunol. 12:701006. 2021.
2. Hashiguchi M, Kobori H, Ritprajak P, et al. Proc Natl Acad Sci USA. 105: 10495–10500. 2008.
3. Nagashima O, Harada N, Usui Y, et al. J Immunol. 181(6):4062-71. 2008.
4. Yamato I, Sho M, Nomi T, et al. Br J Cancer. 101(10):1709-1716. 2009.
5. Kamachi F, Isshiki T, Harada N, et al. Biochem Biophys Res Commun. 463(4):739-45. 2015.
Flow Cytometry

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.