Anti-Mouse CD31 (PECAM-1) – Purified in vivo PLATINUM™ Functional Grade

Mouse 32D leukocyte cell line

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Liquid

Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM^TM antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only

2 – 8° C Wet Ice

390 activity is directed against mouse CD31 (PECAM-1).

PECAM-1 was the first immunoreceptor tyrosine-based inhibition motif (ITIM)-containing receptor to be identified in platelets and acts as a negative regulator of platelet activation¹. Platelet activation from a resting state has broad implications for many pathophysiological processes including anthogenesis, angiogenesis, inflammation, wound repair, and cancer metastasis. PECAM-1 inhibits platelet activation and thrombosis at sites of vascular injury by attenuating immunoreceptor tyrosine-based activation motif (ITAM)-containing receptor complex GPVI-FcR γ-chain signaling and by acting as a positive regulator of α_IIbβ₃-mediated outside-in signaling. PECAM-1, like all ITIM-containing receptors, belongs to the immunoglobulin receptor superfamily.

PECAM-1 also functions as a cell adhesion molecule and is capable of mediating calcium- dependent heterophilic aggregation². Additionally, PECAM-1 is expressed at cell-cell borders in the endocardial cells of the developing murine heart³. Because of its distribution pattern, PECAM-1 is a target molecule for the delivery of antithrombotic agents^4,5,6,7. Research is being conducted into the ability of PECAM-1 to deliver drugs to the endothelium.

Clone 390 was generated by immunizing rats with mouse 32D leukocyte cell line and screening against muPECAM-1Δ12,15^3,8. 390 binds to an epitope on Ig-domain 2 of muPECAM-1^7,9.

PECAM-1 is expressed on the majority of non-erythroid hematopoietic cells, including platelets, monocytes, neutrophils, T cells, and B cell subsets, as well as on vascular endothelium and the endocardial cells of developing heart tissue.

CD38, αV/β3 integrin

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4 Muzykantov VR, Christofidou-Solomidou M, Balyasnikova I, et al. Proc Natl Acad Sci U S A. 96(5):2379-2384. 1999.
5 Scherpereel A, Wiewrodt R, Christofidou-Solomidou M, et al. FASEB J. 15(2):416-426. 2001.
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10 DeLisser HM, Newman PJ, Albelda SM. Immunol Today. 15(10):490-495. 1994.
11 Wee JL, Jackson DE. Blood. 106(12):3816-3823. 2005.
12 Fu T, Sullivan DP, Gonzalez AM, et al. Immunity. 56(10):2311-2324.e6. 2023.