Anti-Mouse CD31 (PECAM-1) [Clone 390] — Purified in vivo PLATINUM™ Functional Grade

Clone 390 is a monoclonal rat antibody that targets mouse CD31 (PECAM-1), and it is widely used in in vivo mouse studies for several key applications:

• In vivo endothelial labeling and imaging: Clone 390 is used to label endothelial cells by intravenous or local injection, enabling visualization and tracking of blood vessels in live mice. For example, fluorescently labeled versions (such as AF488 or RPE conjugates) have been injected into tumor-bearing mice to mark vascular endothelium, allowing imaging by confocal laser endomicroscopy or fluorescence-guided fiber-optic methods. Proper fluorescent signal in perfused mouse liver requires doses of at least 1200 ng (1?µg/g tissue) to distinguish endothelial markers during imaging.

• Functional blockade of CD31: The antibody can be used to block in vivo functions mediated by CD31, such as inhibiting endothelial cell-cell adhesion, and thus is authoritative for studies requiring inhibition of angiogenic or inflammatory signaling mediated by this molecule. This blockade can impact leukocyte transendothelial migration and modulate angiogenesis processes during disease modeling.

• Intravital imaging and immunofluorescence: Clone 390 is also used for intravital microscopy and immunofluorescence to directly visualize blood vessels and endothelial interactions in live or freshly isolated mouse tissues.

• Flow cytometry: Labeled forms of the antibody are used to identify and quantify endothelial and select hematopoietic populations in living mice.

Key details about the antibody:

• Specific for mouse CD31/PECAM-1; recognizes a 130-140?kDa glycoprotein expressed on endothelium, platelets, and some leukocytes.
• Used unconjugated for blocking or with fluorophore conjugates (e.g., AF488, RPE, BUV395) for imaging.
• Shown to be effective for in vivo CD31 blockade, intravital imaging, immunofluorescence, and flow cytometry.

Typical use protocols:

• Injected intravenously (dose varies; for imaging, 5?µg can be used, or at least 1200?ng for liver segment perfusion).
• For blocking studies, dosage and regimen should be optimized based on the desired level of CD31 inhibition and mouse model specifics.

In summary, clone 390 is a versatile tool in mouse in vivo research for labeling, imaging, and functional studies of the vascular endothelium and CD31-mediated processes.

I don't have specific information available about "sterile packaged clone 390" or its required storage temperature. The search results provided contain general information about sterilization processes and biological storage temperatures, but they don't include details about this particular product.

To determine the correct storage temperature for sterile packaged clone 390, you would need to:

Check Product Documentation

• Review the product label or packaging insert
• Consult the manufacturer's instructions for use (IFU)
• Check any accompanying certificates or specification sheets

Contact the Manufacturer

• Reach out to the supplier or manufacturer directly
• Request specific storage and handling requirements
• Verify any temperature-sensitive storage conditions

General ConsiderationsBased on the available information about biological storage, sterile products typically require specific temperature ranges depending on their composition and intended use. Common storage categories include controlled room temperature (20°C to 25°C), refrigerated storage (2°C to 8°C), or various freezer storage options ranging from -20°C to cryogenic temperatures below -150°C.

However, without knowing the specific nature of clone 390 - whether it's a biological sample, pharmaceutical product, or medical device - I cannot provide the exact storage temperature requirements. The correct storage conditions would be critical for maintaining the product's sterility and efficacy, so consulting the official product documentation or manufacturer is essential.

The antibody 390 refers specifically to MSVA-390R, a recombinant rabbit monoclonal antibody directed against myelin basic protein (MBP). In published research, this antibody is most often used to assess myelination in brain tissues or to characterize demyelinating diseases.

When researchers use the 390 (MSVA-390R) antibody, other commonly used antibodies or proteins in the same studies typically include:

• Other anti-MBP antibodies: For validation purposes, the MSVA-390R is often compared directly with other anti-MBP antibodies, such as CAB002300. This allows cross-verification of staining specificity and pattern.

• Markers of other myelin components: Studies involving MBP frequently use antibodies against other myelin or oligodendrocyte proteins, such as:

  • Proteolipid protein 1 (PLP1)
  • Myelin oligodendrocyte glycoprotein (MOG)
  • Oligodendrocyte transcription factor (OLIG2)
  • CNPase (2',3'-cyclic-nucleotide 3'-phosphodiesterase)

• Neuronal and glial markers: To distinguish between cell types and identify demyelination or damage, researchers often include markers such as:

  • Neurofilament proteins
  • GFAP (Glial fibrillary acidic protein) for astrocytes
  • Iba1 or CD68 for microglia/macrophages

• Disease-related proteins: In studies of demyelinating or neurodegenerative disease, antibodies for disease markers (like amyloid-? in Alzheimer's or ?-synuclein in Parkinson’s studies) may be included to correlate MBP alterations with disease pathology.

• Human Protein Atlas validation: MSVA-390R's expression patterns have also been validated against RNA-seq data and other datasets (FANTOM5, GTEx), and are consistent with brain-specific expression.

Summary Table: Common co-used antibodies/proteins with MSVA-390R (anti-MBP 390)

These are representative of those most frequently used in combination with 390 for neurobiological and neuropathological research. If a different "390" antibody or protein was intended in your question, please clarify for a more targeted answer.

No evidence from the available search results indicates a widely recognized or cited "clone 390" entity in the scientific literature across major domains such as molecular biology, clinical research, or systematic reviews. However, there is a mention of "clone Ws23" and "clone Vm10" (not clone 390) in a 2001 bacteriology study focusing on PCR and cloning artifacts in microbial genomic research. This paper discusses sequence aberrations introduced during microbial clone library generation, noting that some clones contain mixed sequences, likely due to heteroduplex formation and polymerase errors, but it does not specify findings or citations related to any entity called "clone 390".

If you are seeking specific key findings for a "clone 390" in a particular context (e.g., cell biology, oncology, immunology), please clarify the domain or species, as no relevant results were found in the provided literature.