Anti-Mouse CD49d – FITC

Anti-Mouse CD49d – FITC

Product No.: C624

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Clone
R1-2
Target
CD49D
Formats AvailableView All
Product Type
Hybridoma Monoclonal Antibody
Alternate Names
VLA-4 α chain, integrin α4, ITGA4
Isotype
Rat IgG2b κ
Applications
FC

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Immunogen
AKR/Cum mouse Spontaneous T lymphoma line TK1
Product Concentration
0.2 mg/ml
Formulation
This Fluorescein (FITC) conjugate is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
State of Matter
Liquid
Storage and Handling
This Fluorescein conjugate is stable when stored at 2-8°C. Do not freeze.
Regulatory Status
Research Use Only
Country of Origin
USA
Shipping
2-8°C Wet Ice
Additional Applications Reported In Literature ?
FC
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
R1-2 activity is directed against mouse CD49d.
Background
Integrins are a large family of heterodimeric transmembrane molecules that mediate adhesion, migration, cell survival, and cell differentiation. CD49d is a single-pass type I membrane glycoprotein also known as integrin alpha-4 (Uniprot Accession P13612). CD49d is the α4 subunit of integrin heterodimers alpha-4/beta-1 (VLA-4; CD49d/CD29; α4β1 integrin) and alph-4/beta-7 (LPAM-1)1. These integrins act as receptors for fibronectin and VCAM1 (CD106). Integrin alpha-4/beta-7 is also a receptor for MADCAM1.

CD49d is expressed on most lymphocytes, granulocytes, monocytes, and thymocytes. CD49d/CD29 (VLA-4; α4β1) is expressed at high levels on the surface of lymphohematopoietic progenitors and is involved in their development and proliferation. CD49d/CD29 integrin/VCAM-1 interactions facilitate B cell adhesion to stromal cells and enhance B cell activation. In the absence of alpha-4 integrins, pre-B cells fail to transmigrate and proliferate.

R1-2 was generated by immunizing Fisher rats with TK1, a Peyer’s patch high endothelial venules (HEV) binding lymphoma line2. Spleen cells were subsequently fused with nonsecreting mouse myeloma P3x63Ag8.653 cells. Hybridomas producing antibodies reactive with TK1 cells, but not the HEV nonbinding lymphoma TK5, were cloned and screened for inhibition of lymphocyte binding to HEV of either peripheral nodes or Peyer’s patches.
Antigen Distribution
CD49d is expressed on T cells, B cells, NK cells, dendritic cells, thymocytes, monocytes, eosinophils, mast cells.

Antigen Details

Ligand/Receptor
VCAM-1, MAdCAM-1, fibronectin
NCBI Gene Bank ID
UniProt.org
Research Area
Cell Adhesion
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Immunology

References & Citations

1. Holzmann B, Weissman IL. EMBO J. 8(6):1735-1741. 1989.
2. Holzmann B, McIntyre BW, Weissman IL. Cell. 56(1):37-46. 1989.
3. Jin H, Aiyer A, Su J, et al. J Clin Invest. 116(3):652-662. 2006.
4. DeNucci CC, Pagán AJ, Mitchell JS, et al. J Immunol. 184(5):2458-2467. 2010.
5. Uchida Y, Kawai K, Ibusuki A, et al. J Immunol. 186(12):6945-6954. 2011.
6. Hadeiba H, Lahl K, Edalati A, et al. Immunity. 36(3):438-450. 2012.
7. Shokeen M, Zheleznyak A, Wilson JM, et al. J Nucl Med. 53(5):779-786. 2012.
8. Renkema KR, Li G, Wu A, et al. J Immunol. 192(1):151-159. 2014.
9. Hermida MD, Doria PG, Taguchi AM, et al. BMC Infect Dis. 14:450. 2014.
10. Mamedov MR, Scholzen A, Nair RV, et al. Immunity. 48(2):350-363.e7. 2018.
11. Rolot M, Dougall AM, Chetty A, et al. Nat Commun. 9(1):4516. 2018.
12. Martin MD, Sompallae R, Winborn CS, et al. Cell Rep. 31(2):107508. 2020.
13. Müller K, Gibbins MP, Roberts M, et al. EMBO Mol Med. 13(4):e13390. 2021.
14. Barrett SP, Riordon A, Toh BH, et al. J Leukoc Biol. 67(2):169-173. 2000.
15. Lin J, Qin L, Chavin KD, et al. Pathobiology. 63(3):119-132. 1995.
16. Chisholm PL, Williams CA, Lobb RR. Eur J Immunol. 23(3):682-688. 1993.
Flow Cytometry

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.