Anti-Mouse CD90.2 (Thy 1.2) [Clone 30-H12] — Purified in vivo PLATINUM™ Functional Grade

Anti-Mouse CD90.2 (Thy 1.2) [Clone 30-H12] — Purified in vivo PLATINUM™ Functional Grade

Product No.: C430

[product_table name="All Top" skus="C430"]

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Clone
30-H12
Target
CD90.2
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
Thy-1.2
Isotype
Rat IgG2b κ
Applications
Costim
,
CyTOF®
,
Depletion
,
FC
,
IHC FF
,
in vivo
,
PhenoCycler®
,
WB

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Data

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Antibody Details

Product Details

Reactive Species
Mouse
Host Species
Rat
Recommended Isotype Controls
Recommended Dilution Buffer
Immunogen
Mouse thymus or spleen
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
FC The suggested concentration for this 30-H12 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl or 100 μl of whole blood. Titration of the reagent is recommended for optimal performance for each application.
WB The suggested concentration for this 30-H12 antibody for use in western blotting is 1-10 μg/ml.
Additional Applications Reported In Literature ?
CODEX®
CyTOF®
IHC (Frozen)
Costim
Depletion
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Description

Specificity
Clone 30-H12 recognizes the Thy-1.2 leukocyte marker.
Background
CD90 is a 28-30 kD GPI-linked membrane glycoprotein and is part of the Ig superfamily. It interacts with CD45 in signal transduction. CD90 mediates adhesion of thymocytes to thymic stroma. It has been reported that CD90 binds with β2 and β3 integrins and is involved in the inhibition of hematopoietic stem cells proliferation and differentiation, as well as the regulation of cell adhesion and neurite outgrowth. It can be used as a marker for various stem cells, such as hematopoietic stem cells, and for the axonal processes of mature neurons. For use in FACS, CD90 is a popular surface marker for stem cells in combination with other markers such as CD34. There are two alleles for CD90 in mice that differ by one amino acid. The difference being that CD90.1 (Thy1.1) has an arginine and CD90.2 (Thy1.2) has a glutamine at position 108. CD90.2 is more prevalent and is expressed in most mice strains. CD90.1 is only expressed by a select few mice strains including AKR/J and PL strains. CD90.2 is a 25-35 kD GPI-anchored membrane glycoprotein. Like CD90, it is also in the Ig superfamily, interacts with CD45, and has involvement in signal transduction. The function of CD90.2 is thought to play roles in cognition, axon growth, T lymphocyte function, and apoptosis. CD90 acts as tumor suppressor for some tumors due to its action in upregulating thrombospondin, SPARC (osteonectin), and fibronectin. On the other hand, it has been suspected to aid in the spread of circulating melanoma cells. Regarding prostate cancer, CD90 has therapeutic potential for specific drug targeting due to its expression in cancer associated stroma, but not in normal stroma.
Antigen Distribution
CD90.2 is present on hematopoietic stem cells and neurons, all thymocytes, peripheral T cells of the Thy-1.2 bearing mice.
Ligand/Receptor
CD45
PubMed
NCBI Gene Bank ID
Research Area
Immunology

Leinco Antibody Advisor

Powered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments.

Clone 30-H12 is most commonly used in vivo in mice to deplete T lymphocytes and to distinguish cell populations expressing the CD90.2 (Thy1.2) marker, particularly T cells and hematopoietic stem cells.

Key in vivo applications include:

  • T cell depletion: 30-H12 is widely used to selectively deplete T cells in mice expressing the Thy1.2 allele (e.g., C57BL/6, BALB/c), facilitating studies of immune function, transplantation, and graft-versus-host disease. This application is central for experiments requiring immune modulation or removal of T cells to analyze their role in disease or therapy.
  • In vivo cell tracking, targeting, and sorting: Because the antibody specifically binds to CD90.2, it allows for tracking, isolation, and identification of Thy1.2-expressing cell populations, including T lymphocytes, hematopoietic stem cells, and some neural cells.
  • Functional assays: 30-H12 can induce calcium flux in thymocytes and is used for functional studies involving T cell activation, apoptosis, or inhibition of T cell proliferation in vivo.
  • Protection studies: It is used to assess dependency on T cells in various immunization or challenge protocols by depleting the T cell population and observing changes in immune responses or disease outcomes (for example, monitoring graft-versus-host disease or evaluating vaccine efficacy).

Additional points:

  • Clone 30-H12 does not cross-react with Thy1.1 (AKR/J, PL strains), making it strain-selective.
  • The antibody is reported for use in both acute and long-term T cell depletion regimens in immune modulation studies.
  • Besides in vivo uses, 30-H12 is also widely used for flow cytometry and immunohistochemical analysis of mouse lymphoid organs.

Summary:
The primary in vivo application of clone 30-H12 in mice is depletion of Thy1.2-positive T lymphocytes, enabling immune-modulation studies, with secondary uses in tracking and identifying Thy1.2-positive cell populations.

The 30-H12 antibody targets mouse CD90.2 (Thy1.2) and is commonly used to identify or deplete T cells, particularly in flow cytometry and in vivo cell-depletion studies. In literature, 30-H12 is rarely used alone and is frequently combined with other antibodies or proteins to distinguish cell populations or track immune responses.

Commonly co-used antibodies and proteins with 30-H12 include:

  • CD3 (pan-T cell marker): Helps in identifying all T cells.
  • CD4 and CD8: To further subdivide T cells into helper (CD4+) and cytotoxic (CD8+) subsets.
  • CD45: A general leukocyte (white blood cell) marker.
  • CD44, CD62L: Markers for T cell activation or memory status.
  • CD25: Activation marker or regulator T cell marker.
  • B220/CD19: B cell markers, to distinguish T cells (CD90.2+) from B cells (CD90.2-).
  • CD11b, Gr-1: Markers for myeloid lineage cells.
  • Sca-1, c-Kit: For identification of hematopoietic stem and progenitor cells, especially in mouse bone marrow studies.
  • NK1.1: For excluding or studying NK (natural killer) cells.
  • FOXP3 (intracellular): Regulatory T cell marker, often used in combination with CD4, CD25, and CD90.2.

In flow cytometry panels, it is standard to co-stain CD90.2 with the above antibodies to define T cell subsets, distinguish T cells from other immune or stromal cells, or identify stem cells in non-lymphoid tissues. These markers are cited in antibody company product pages and usage notes, which often discuss paired markers for typical flow or in vivo depletion experiments.

In summary, CD3, CD4, CD8, CD25, CD44, CD62L, CD45, B220/CD19, Sca-1, c-Kit, CD11b, Gr-1, and NK1.1 are some of the most commonly used antibodies or proteins together with 30-H12 in mouse immunology research.

Key findings from scientific literature citing clone 30-H12 primarily relate to its fundamental role as an antibody probe against murine CD90.2 (Thy-1.2), with significant impact in immunology, stem cell, and neuroscience research.

Main findings include:

  • Specificity: Clone 30-H12 specifically recognizes CD90.2, a glycoprotein present on most mouse thymocytes, T lymphocytes, intraepithelial lymphocytes, some neuronal and stromal cells, and hematopoietic stem cells. It does not cross-react with Thy-1.1 or rat Thy-1, making it valuable for studies distinguishing mouse strains or cell populations.

  • Signal transduction and cellular interaction: Studies using 30-H12 reveal that CD90.2 interacts with CD45 and is involved in signal transduction, particularly in T cells and thymocytes. It mediates cell adhesion (notably thymocyte-stroma interactions) and transduces signals that can modulate cell fate.

  • Functional manipulation of T cells:

    • Depletion and subset targeting: 30-H12 is routinely used in vivo and in vitro to deplete or identify T-cell populations, helping dissect T-cell contributions in immunity and disease models.
    • Flow cytometry: Widely adopted for flow cytometric quantification and isolation of murine T cell subsets, neural cells, and stem cells.
  • Impact on T-cell function and apoptosis:

    • Crosslinking 30-H12 on thymocytes induces Ca²⁺ influx; co-crosslinking with CD3/TCR enhances signal transduction, can trigger apoptosis, or inhibit CD3-mediated proliferation in mature T lymphocytes.
    • These manipulations assist in studying thymocyte development, TCR signaling, and programmed cell death.
  • Applications in disease models:

    • Depletion with 30-H12 antibody in animal models (e.g., mice infected with M. tuberculosis) provides direct evidence for the requirement of Thy-1.2⁺ T cells in vaccine-mediated protection.
    • Used for tracking T-cell subpopulations during immune responses, tissue regeneration, or neurobiological events.

Additional notes:

  • CD90.2 function in cell-cell interactions during brain development and tissue regeneration is also a research focus, extending applications beyond immunology.
  • The antibody is considered a gold standard for murine T-cell identification due to its consistent performance and lack of cross-reactivity with related antigens.

In summary, clone 30-H12 citations underscore its dual value as a phenotyping tool and a functional probe for dissecting mouse immune system components and mechanisms.

Clone 30-H12, an anti-mouse CD90.2 (Thy1.2) monoclonal antibody, is primarily used for T lymphocyte depletion in mouse models, with dosing regimens varying based on the specific research application and experimental design.

T Cell Depletion Studies

In protective immunity research, the 30-H12 antibody has been administered intraperitoneally with varying schedules depending on the experimental timeline. In one tuberculosis vaccination study, mice received anti-Thy-1.2 (clone 30-H12) treatment twice before challenge and then every 10 days following challenge. This schedule demonstrates a maintenance approach where initial depletion is established before pathogen exposure, followed by regular dosing to sustain T cell depletion throughout the observation period.

General Dosing Considerations

The specific dose amounts for clone 30-H12 are tailored to different mouse strains and research applications. While the search results don't provide exact microgram amounts for 30-H12 specifically, dosing strategies for similar depletion antibodies in mouse models typically follow certain patterns. The antibody is administered via intraperitoneal injection, which is the standard route for most in vivo antibody studies in mice.

Application-Specific Variations

Clone 30-H12's utility extends beyond simple depletion studies. The antibody has been reported to induce calcium flux in thymocytes, suggesting it can modulate T cell function in addition to depleting T cell populations. This functional capability means dosing may need adjustment depending on whether the goal is complete depletion or functional modulation.

The antibody's effectiveness is also strain-specific, as Thy1.2 is only expressed in certain mouse strains including C57BL/6, BALB/c, CBA, C3H, C58/, SJL, DBA, and NZB/. This strain restriction necessitates careful consideration when designing experiments and selecting appropriate dosing regimens.

References & Citations

1. Ledbetter, J. and Herzenberg, L. (1979) Immunol. Rev. 47:63
2. Unkeless, JC. (1979) J. Exp. Med. 150:580
Costim
CyTOF®
Depletion
Flow Cytometry
IHC FF
in vivo Protocol
PhenoCycler®
General Western Blot Protocol

Certificate of Analysis

Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.