Anti-Mouse CD90.2 (Thy 1.2) [Clone 30-H12] — Purified in vivo PLATINUM™ Functional Grade

Clone 30-H12 is most commonly used in vivo in mice to deplete T lymphocytes and to distinguish cell populations expressing the CD90.2 (Thy1.2) marker, particularly T cells and hematopoietic stem cells.

Key in vivo applications include:

• T cell depletion: 30-H12 is widely used to selectively deplete T cells in mice expressing the Thy1.2 allele (e.g., C57BL/6, BALB/c), facilitating studies of immune function, transplantation, and graft-versus-host disease. This application is central for experiments requiring immune modulation or removal of T cells to analyze their role in disease or therapy.
• In vivo cell tracking, targeting, and sorting: Because the antibody specifically binds to CD90.2, it allows for tracking, isolation, and identification of Thy1.2-expressing cell populations, including T lymphocytes, hematopoietic stem cells, and some neural cells.
• Functional assays: 30-H12 can induce calcium flux in thymocytes and is used for functional studies involving T cell activation, apoptosis, or inhibition of T cell proliferation in vivo.
• Protection studies: It is used to assess dependency on T cells in various immunization or challenge protocols by depleting the T cell population and observing changes in immune responses or disease outcomes (for example, monitoring graft-versus-host disease or evaluating vaccine efficacy).

Additional points:

• Clone 30-H12 does not cross-react with Thy1.1 (AKR/J, PL strains), making it strain-selective.
• The antibody is reported for use in both acute and long-term T cell depletion regimens in immune modulation studies.
• Besides in vivo uses, 30-H12 is also widely used for flow cytometry and immunohistochemical analysis of mouse lymphoid organs.

Summary:
The primary in vivo application of clone 30-H12 in mice is depletion of Thy1.2-positive T lymphocytes, enabling immune-modulation studies, with secondary uses in tracking and identifying Thy1.2-positive cell populations.

The 30-H12 antibody targets mouse CD90.2 (Thy1.2) and is commonly used to identify or deplete T cells, particularly in flow cytometry and in vivo cell-depletion studies. In literature, 30-H12 is rarely used alone and is frequently combined with other antibodies or proteins to distinguish cell populations or track immune responses.

Commonly co-used antibodies and proteins with 30-H12 include:

• CD3 (pan-T cell marker): Helps in identifying all T cells.
• CD4 and CD8: To further subdivide T cells into helper (CD4+) and cytotoxic (CD8+) subsets.
• CD45: A general leukocyte (white blood cell) marker.
• CD44, CD62L: Markers for T cell activation or memory status.
• CD25: Activation marker or regulator T cell marker.
• B220/CD19: B cell markers, to distinguish T cells (CD90.2+) from B cells (CD90.2-).
• CD11b, Gr-1: Markers for myeloid lineage cells.
• Sca-1, c-Kit: For identification of hematopoietic stem and progenitor cells, especially in mouse bone marrow studies.
• NK1.1: For excluding or studying NK (natural killer) cells.
• FOXP3 (intracellular): Regulatory T cell marker, often used in combination with CD4, CD25, and CD90.2.

In flow cytometry panels, it is standard to co-stain CD90.2 with the above antibodies to define T cell subsets, distinguish T cells from other immune or stromal cells, or identify stem cells in non-lymphoid tissues. These markers are cited in antibody company product pages and usage notes, which often discuss paired markers for typical flow or in vivo depletion experiments.

In summary, CD3, CD4, CD8, CD25, CD44, CD62L, CD45, B220/CD19, Sca-1, c-Kit, CD11b, Gr-1, and NK1.1 are some of the most commonly used antibodies or proteins together with 30-H12 in mouse immunology research.

Key findings from scientific literature citing clone 30-H12 primarily relate to its fundamental role as an antibody probe against murine CD90.2 (Thy-1.2), with significant impact in immunology, stem cell, and neuroscience research.

Main findings include:

• Specificity: Clone 30-H12 specifically recognizes CD90.2, a glycoprotein present on most mouse thymocytes, T lymphocytes, intraepithelial lymphocytes, some neuronal and stromal cells, and hematopoietic stem cells. It does not cross-react with Thy-1.1 or rat Thy-1, making it valuable for studies distinguishing mouse strains or cell populations.

• Signal transduction and cellular interaction: Studies using 30-H12 reveal that CD90.2 interacts with CD45 and is involved in signal transduction, particularly in T cells and thymocytes. It mediates cell adhesion (notably thymocyte-stroma interactions) and transduces signals that can modulate cell fate.

• Functional manipulation of T cells:

  • Depletion and subset targeting: 30-H12 is routinely used in vivo and in vitro to deplete or identify T-cell populations, helping dissect T-cell contributions in immunity and disease models.
  • Flow cytometry: Widely adopted for flow cytometric quantification and isolation of murine T cell subsets, neural cells, and stem cells.

• Impact on T-cell function and apoptosis:

  • Crosslinking 30-H12 on thymocytes induces Ca²⁺ influx; co-crosslinking with CD3/TCR enhances signal transduction, can trigger apoptosis, or inhibit CD3-mediated proliferation in mature T lymphocytes.
  • These manipulations assist in studying thymocyte development, TCR signaling, and programmed cell death.

• Applications in disease models:

  • Depletion with 30-H12 antibody in animal models (e.g., mice infected with M. tuberculosis) provides direct evidence for the requirement of Thy-1.2⁺ T cells in vaccine-mediated protection.
  • Used for tracking T-cell subpopulations during immune responses, tissue regeneration, or neurobiological events.

Additional notes:

• CD90.2 function in cell-cell interactions during brain development and tissue regeneration is also a research focus, extending applications beyond immunology.
• The antibody is considered a gold standard for murine T-cell identification due to its consistent performance and lack of cross-reactivity with related antigens.

In summary, clone 30-H12 citations underscore its dual value as a phenotyping tool and a functional probe for dissecting mouse immune system components and mechanisms.

Clone 30-H12, an anti-mouse CD90.2 (Thy1.2) monoclonal antibody, is primarily used for T lymphocyte depletion in mouse models, with dosing regimens varying based on the specific research application and experimental design.

T Cell Depletion Studies

In protective immunity research, the 30-H12 antibody has been administered intraperitoneally with varying schedules depending on the experimental timeline. In one tuberculosis vaccination study, mice received anti-Thy-1.2 (clone 30-H12) treatment twice before challenge and then every 10 days following challenge. This schedule demonstrates a maintenance approach where initial depletion is established before pathogen exposure, followed by regular dosing to sustain T cell depletion throughout the observation period.

General Dosing Considerations

The specific dose amounts for clone 30-H12 are tailored to different mouse strains and research applications. While the search results don't provide exact microgram amounts for 30-H12 specifically, dosing strategies for similar depletion antibodies in mouse models typically follow certain patterns. The antibody is administered via intraperitoneal injection, which is the standard route for most in vivo antibody studies in mice.

Application-Specific Variations

Clone 30-H12's utility extends beyond simple depletion studies. The antibody has been reported to induce calcium flux in thymocytes, suggesting it can modulate T cell function in addition to depleting T cell populations. This functional capability means dosing may need adjustment depending on whether the goal is complete depletion or functional modulation.

The antibody's effectiveness is also strain-specific, as Thy1.2 is only expressed in certain mouse strains including C57BL/6, BALB/c, CBA, C3H, C58/, SJL, DBA, and NZB/. This strain restriction necessitates careful consideration when designing experiments and selecting appropriate dosing regimens.