Anti-Mouse CD90.2 (Thy 1.2) [Clone 30-H12] — Purified in vivo PLATINUM™ Functional Grade
Anti-Mouse CD90.2 (Thy 1.2) [Clone 30-H12] — Purified in vivo PLATINUM™ Functional Grade
Product No.: C430
|  Clone 30-H12 Target		 CD90.2  Formats AvailableView All  Product Type Monoclonal Antibody  Alternate Names  Thy-1.2 Isotype		 Rat IgG2b κ Applications		 Costim ,  CyTOF® ,  Depletion ,  FC ,  IHC FF ,  in vivo ,  PhenoCycler® ,  WB | 
Data
| Antibody DetailsProduct DetailsReactive Species		 Mouse Host Species		 Rat Recommended Isotype Controls Recommended Dilution Buffer Immunogen Mouse thymus or spleen Product Concentration		 ≥ 5.0 mg/ml Endotoxin Level		 <0.5 EU/mg as determined by the LAL method Purity		 ≥98% monomer by analytical SEC  ⋅  >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.  Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C.  Avoid Repeated Freeze Thaw Cycles.  Country of Origin		 USA Shipping Next Day 2-8°C RRIDAB_2829713 Applications and Recommended Usage? Quality Tested by Leinco FC The suggested concentration for this 30-H12 antibody for staining cells in flow cytometry is ≤ 0.25 μg per 106 cells in a volume of 100 μl or 100 μl of whole blood. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for this 30-H12 antibody for use in western blotting is 1-10 μg/ml. Additional Applications Reported In Literature ?  CODEX® CyTOF® IHC (Frozen) Costim Depletion Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.  DescriptionDescriptionSpecificity Clone 30-H12 recognizes the Thy-1.2 leukocyte marker. Background CD90 is a 28-30 kD GPI-linked membrane glycoprotein and is part of the Ig superfamily. It interacts with CD45 in signal transduction. CD90 mediates adhesion of thymocytes to thymic stroma. It has been reported that CD90 binds with β2 and β3 integrins and is involved in the inhibition of hematopoietic stem cells proliferation and differentiation, as well as the regulation of cell adhesion and neurite outgrowth. It can be used as a marker for various stem cells, such as hematopoietic stem cells, and for the axonal processes of mature neurons. For use in FACS, CD90 is a popular surface marker for stem cells in combination with other markers such as CD34. There are two alleles for CD90 in mice that differ by one amino acid. The difference being that CD90.1 (Thy1.1) has an arginine and CD90.2 (Thy1.2) has a glutamine at position 108. CD90.2 is more prevalent and is expressed in most mice strains. CD90.1 is only expressed by a select few mice strains including AKR/J and PL strains. CD90.2 is a 25-35 kD GPI-anchored membrane glycoprotein. Like CD90, it is also in the Ig superfamily, interacts with CD45, and has involvement in signal transduction. The function of CD90.2 is thought to play roles in cognition, axon growth, T lymphocyte function, and apoptosis. CD90 acts as tumor suppressor for some tumors due to its action in upregulating thrombospondin, SPARC (osteonectin), and fibronectin. On the other hand, it has been suspected to aid in the spread of circulating melanoma cells. Regarding prostate cancer, CD90 has therapeutic potential for specific drug targeting due to its expression in cancer associated stroma, but not in normal stroma.  Antigen Distribution CD90.2 is present on hematopoietic stem cells and neurons, all thymocytes, peripheral T cells of the Thy-1.2 bearing mice. Ligand/Receptor CD45 PubMed NCBI Gene Bank ID UniProt.org Research Area		 Immunology Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone 30-H12 is most commonly used in vivo in mice to deplete T lymphocytes and to distinguish cell populations expressing the CD90.2 (Thy1.2) marker, particularly T cells and hematopoietic stem cells. Key in vivo applications include: 
 Additional points: 
 Summary: The 30-H12 antibody targets mouse CD90.2 (Thy1.2) and is commonly used to identify or deplete T cells, particularly in flow cytometry and in vivo cell-depletion studies. In literature, 30-H12 is rarely used alone and is frequently combined with other antibodies or proteins to distinguish cell populations or track immune responses. Commonly co-used antibodies and proteins with 30-H12 include: 
 In flow cytometry panels, it is standard to co-stain CD90.2 with the above antibodies to define T cell subsets, distinguish T cells from other immune or stromal cells, or identify stem cells in non-lymphoid tissues. These markers are cited in antibody company product pages and usage notes, which often discuss paired markers for typical flow or in vivo depletion experiments. In summary, CD3, CD4, CD8, CD25, CD44, CD62L, CD45, B220/CD19, Sca-1, c-Kit, CD11b, Gr-1, and NK1.1 are some of the most commonly used antibodies or proteins together with 30-H12 in mouse immunology research. Key findings from scientific literature citing clone 30-H12 primarily relate to its fundamental role as an antibody probe against murine CD90.2 (Thy-1.2), with significant impact in immunology, stem cell, and neuroscience research. Main findings include: 
 Additional notes: 
 In summary, clone 30-H12 citations underscore its dual value as a phenotyping tool and a functional probe for dissecting mouse immune system components and mechanisms. Clone 30-H12, an anti-mouse CD90.2 (Thy1.2) monoclonal antibody, is primarily used for T lymphocyte depletion in mouse models, with dosing regimens varying based on the specific research application and experimental design. T Cell Depletion StudiesIn protective immunity research, the 30-H12 antibody has been administered intraperitoneally with varying schedules depending on the experimental timeline. In one tuberculosis vaccination study, mice received anti-Thy-1.2 (clone 30-H12) treatment twice before challenge and then every 10 days following challenge. This schedule demonstrates a maintenance approach where initial depletion is established before pathogen exposure, followed by regular dosing to sustain T cell depletion throughout the observation period. General Dosing ConsiderationsThe specific dose amounts for clone 30-H12 are tailored to different mouse strains and research applications. While the search results don't provide exact microgram amounts for 30-H12 specifically, dosing strategies for similar depletion antibodies in mouse models typically follow certain patterns. The antibody is administered via intraperitoneal injection, which is the standard route for most in vivo antibody studies in mice. Application-Specific VariationsClone 30-H12's utility extends beyond simple depletion studies. The antibody has been reported to induce calcium flux in thymocytes, suggesting it can modulate T cell function in addition to depleting T cell populations. This functional capability means dosing may need adjustment depending on whether the goal is complete depletion or functional modulation. The antibody's effectiveness is also strain-specific, as Thy1.2 is only expressed in certain mouse strains including C57BL/6, BALB/c, CBA, C3H, C58/, SJL, DBA, and NZB/. This strain restriction necessitates careful consideration when designing experiments and selecting appropriate dosing regimens. References & Citations1. Ledbetter, J. and Herzenberg, L. (1979) Immunol. Rev. 47:63 2. Unkeless, JC. (1979) J. Exp. Med. 150:580 Technical ProtocolsCertificate of Analysis | 
Formats Available
| Prod No. | Description | 
|---|---|
| C1698 | |
| C1700 | |
| C283 | |
| C286 | |
| C285 | |
| C357 | |
| C284 | |
| C1692 | |
| C1693 | |
| C1694 | |
| C1696 | |
| C521 | |
| C393 | |
| C430 | 
 Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.

 
	
		 
		




