Anti-Mouse CD90 (Thy-1) (Clone M5/49.4.1) – Purified in vivo GOLD™ Functional Grade
Anti-Mouse CD90 (Thy-1) (Clone M5/49.4.1) – Purified in vivo GOLD™ Functional Grade
Product No.: C640
Clone M5/49.4.1 Target Thy1 (CD90) Formats AvailableView All Product Type Hybridoma Monoclonal Antibody Alternate Names Thy-1 membrane glycoprotein, CD90 Isotype Rat IgG2a Applications Depletion , FA , IP |
Antibody DetailsProduct DetailsHost Species Rat Recommended Dilution Buffer Immunogen Secondary mixed lymphocyte culture consisting of C57BL/6 mouse anti-irradiated BN rat lymphoma. Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. State of Matter Liquid Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Regulatory Status Research Use Only Country of Origin USA Shipping 2 – 8° C Wet Ice Additional Applications Reported In Literature ? Depletion, FA, IP Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity M5/49.4.1 activity is directed against mouse Thy1 (CD90). Background Thy1 is a highly conserved, GPI-linked member of the immunoglobulin superfamily that is
important in the immune and nervous systems1 and involved in T-cell activation and cell-cell
interactions2. The effects of Thy1 are context-dependent1. Thy1 is heavily N-glycosylated with a
carbohydrate content of up to 40% of its molecular mass, and its moiety composition varies
between tissues as well as between cells of the same lineage in different stages of differentiation.
Additionally, Thy1 is found in both membrane-bound and soluble forms, and, in mice, Thy1 is
encoded by two alleles, Thy1.1 and Thy1.2, which are distinguished by a single amino acid at
position 891. Thy1 deficiency does not compromise immunity2, but its presence or absence
modulates the phenotypes of certain cancers, fibrotic diseases, and neuronal injury1. Thy1.1 is an
alloantigen of the AKR/J and PL mouse strains, whereas Thy1.2 is expressed by most mouse
strains2,3. M5/49.4.1 was generated by immunizing (Lewis x BN) F1 rats with a secondary mixed lymphocyte culture consisting of C57BL/6 mouse anti-irradiated BN rat lymphoma4. Spleen cells were fused with NS-1 myeloma cells and hybridomas grown and assessed for binding to cytolytic T lymphocyte (CTL) surface molecules. Antigen Distribution CD90 is expressed in mice by thymocytes, peripheral T-cells, myoblasts,
epidermal cells, and keratinocytes. CD90 is considered a pan T cell marker of mice. Ligand/Receptor CD45 NCBI Gene Bank ID Research Area Immunology . Signal Transduction . Immunoglobulins Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. The clone M5/49.4.1 is a rat monoclonal antibody that specifically targets mouse CD90 (Thy-1), a GPI-anchored protein belonging to the immunoglobulin superfamily. This antibody is commonly used in in vivo mouse studies for several applications: Applications of Clone M5/49.4.1
Preparation and Use
Overall, the M5/49.4.1 clone is a versatile tool for researchers studying immune cell dynamics and functions in mouse models. The correct storage temperature for the sterile packaged clone M5/49.4.1, specifically for APC Anti-Mouse CD90 Antibody, is 2-8°C. It is important not to freeze the product and to protect it from prolonged exposure to light. Based on the available information, M5/49.4.1 is an anti-mouse CD90 (Thy1) antibody that is commonly used in research applications, particularly for in vivo depletion studies and immune cell analysis. While the search results don't provide extensive details about specific antibodies commonly co-used with M5/49.4.1, I can outline the typical research contexts and related reagents based on the available data. Primary Applications and ContextM5/49.4.1 is primarily used for T cell depletion studies and targets the Thy1 (CD90) surface marker. This antibody is frequently employed in bone marrow chimera preparation and immune cell depletion experiments. Related Cell Depletion AntibodiesIn the context of immune cell depletion studies where M5/49.4.1 might be used, researchers commonly employ other depletion antibodies targeting different cell populations: T Cell-Related Antibodies:
Other Immune Cell Depletion Antibodies:
Complement and Detection ReagentsSince M5/49.4.1 is used for depletion studies, it's commonly used with complement to enhance cell lysis, as mentioned in bone marrow chimera preparation protocols. The antibody is available in different formats including PE-conjugated versions for flow cytometry applications. Research ApplicationsThe antibody is frequently used in studies involving antigen-presenting cells and cytotoxic T lymphocyte responses, particularly in viral infection models studying LCMV and influenza virus. In these contexts, it would be used alongside various viral antigens and peptide epitopes for immune response analysis. Unfortunately, the search results don't provide comprehensive information about specific antibody combinations or detailed co-usage patterns with M5/49.4.1 in published literature. For more detailed information about commonly co-used antibodies, reviewing specific research papers that cite M5/49.4.1 usage would be necessary. Based on the available search results, clone M5/49.4.1 is an anti-mouse CD90 (Thy-1) antibody clone that appears to be widely used in immunological research, though the search results provide limited specific citations of key scientific findings directly attributed to this particular clone. Basic Characteristics of Clone M5/49.4.1Clone M5/49.4.1 is a rat IgG2a antibody that targets mouse CD90 (Thy-1), a cell surface glycoprotein. The antibody is commercially available from multiple suppliers and has been verified for use in flow cytometry applications, with recommended dilutions of 2 ?g/mL for 1×10?-5×10? cells. It has been validated using C57/BL6 mouse splenocytes. Research ApplicationsThe clone appears in antibody databases and commercial listings maintained by established research institutions, suggesting its reliability and widespread use in the scientific community. The Thy-1/CD90 molecule that this antibody targets is an important cell surface marker used to identify various cell populations, particularly T cells and other immune cell subsets. Limited Specific Literature FindingsWhile the search results confirm that M5/49.4.1 is a well-established research tool, they do not provide specific citations or detailed findings from studies that have directly utilized this particular clone. The antibody appears in commercial catalogs and antibody databases, indicating its commercial availability and presumed utility, but the key scientific discoveries or specific experimental results obtained using this clone are not detailed in the available search results. The search results suggest that this clone has been used in flow cytometry applications for immunophenotyping studies, but without access to the actual research papers that cite this antibody, it's not possible to provide specific findings about what discoveries have been made using clone M5/49.4.1 in particular versus other anti-CD90 antibodies. To obtain comprehensive information about the key scientific findings from studies using clone M5/49.4.1, a more targeted literature search focusing on research papers that specifically cite this clone would be necessary. References & Citations1 Bradley JE, Ramirez G, Hagood JS. Biofactors. 35(3):258-265. 2009. 2 Haeryfar SM, Hoskin DW. J Immunol. 173(6):3581-3588. 2004. 3 Rege TA, Hagood JS. FASEB J. 20(8):1045-1054. 2006. 4 Davignon D, Martz E, Reynolds T, et al. Proc Natl Acad Sci U S A. 78(7):4535-4539. 1981. 5 Weng X, Liao CM, Bagchi S, et al. Eur J Immunol. 44(12):3646-3657. 2014. Technical ProtocolsDepletion FA  Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
