Anti-Mouse CD90 (Thy-1) (Clone T24/31) – Purified in vivo PLATINUM™ Functional Grade

Clone T24/31 is an anti-mouse CD90 (also known as Thy1) monoclonal antibody used in in vivo mouse studies primarily for the depletion of T cells. Researchers administer T24/31 to mice, typically by intraperitoneal injection, to reduce or deplete the population of T cells in order to study their function in various immunological contexts.

Key details:

• Usage: T24/31 is used to deplete T cells in mice to evaluate the role of T cells in immune responses, disease models, or infection.
• Typical protocol: Mice are injected with 250??g of anti-Thy1 (clone T24/31) intraperitoneally, starting one day before experimental induction (such as infection) and every two days thereafter until tissue harvest.
• Effectiveness: Depletion regimens with T24/31 routinely result in an 85–90% reduction in CD4+ and CD8+ T cells, verified by flow cytometry from harvested tissues.
• Applications: These protocols are fundamental in research where T cell function needs to be selectively eliminated, such as studies on viral infection, immune tolerance, or inflammation.

T24/31 is considered an established tool for specific T cell depletion in vivo and is chosen for its effectiveness in removing both CD4+ and CD8+ T cells, thus allowing functional studies of these populations' roles in health and disease.

Based on the search results provided, I cannot find specific information about a sterile packaged clone T24/31. The search results mention a T24 cloning system from TurboKlone, but this appears to be a plant cloning machine rather than a sterile medical device, and no specific storage temperatures are mentioned for it.

General Storage Temperature Guidelines for Sterile Medical Devices

For sterile medical devices in general, the search results indicate that proper storage conditions are critical for maintaining sterility. Storage locations should have a maximum room temperature of 25°C (77°F) and must be dry, clean, protected from light and dust, and free from all unsterile objects.

Storage Duration Considerations

The permissible storage duration for sterile medical devices depends on actual storage conditions rather than just manufacturer specifications. The storage environment must be assessed by relevant hygiene commissions and specified in writing within hygiene plans. Some sterile instruments in single packaging must be used within 24 hours if storage conditions are not optimal.

Recommendation

Without specific information about the T24/31 clone you're referencing, I recommend checking the manufacturer's labeling and packaging for storage temperature requirements. If this is indeed a sterile medical device, it should display appropriate storage symbols and temperature ranges according to ISO standards. For any sterile medical product, maintaining temperatures at or below 25°C in controlled, clean environments is typically required for long-term storage.

Studies using T24/31 antibodies, which target transthyretin (TTR) amyloid in amyloidosis research, commonly pair them with other antibodies or proteins to detect, quantify, or localize TTR and related pathological species. Based on the available literature and practices in amyloid research, the following are among the most commonly used:

• Anti-TTR antibodies: Other monoclonal or polyclonal antibodies specific for TTR, such as those targeting different epitopes (e.g., 115–124, 118–122). The T24 antibody itself targets residues 118–122, and its humanized form, RT24, is sometimes used in parallel or for confirmatory studies on deposits, aggregation, and antibody specificity.

• Patient mutation-targeting antibodies: Mutations like V30M, Y114C, A120S, and V122I—which are linked to familial amyloid polyneuropathy—are sometimes the subject of antibodies specific for the mutated residues, used to compare reactivity or evaluate pathogenicity in experimental constructs.

• Amyloid structure or conformation-specific antibodies: These detect generic features or conformation of amyloid deposits, such as oligomeric or fibrillar arrangements; antibodies that distinguish between aggregated and monomeric TTR are sometimes employed for specificity controls.

• Coomassie Brilliant Blue Staining and Western Blot Controls: While not antibodies, these protein detection methods often accompany immunoblotting to validate antibody specificity and loading efficiency.

Beyond these TTR-specific reagents, broader amyloid research sometimes incorporates:

• Serum amyloid P component (SAP) antibodies: Used to distinguish between different types of amyloid deposits, though not directly paired with T24/31 in most TTR studies.
• Secondary antibodies: For detection in ELISA or Western blotting, including HRP-conjugated anti-mouse or anti-human IgG.

In related fields, bispecific antibodies, antibody–cytokine fusion proteins (immunocytokines like IL-2, IL-12, or TNF), engineered antibody fragments (Fab, scFv), and diagnostic scaffold proteins (affibodies, DARPins, monobodies) are now increasingly common, though their routine use with T24/31 in TTR amyloidosis-specific research is not yet broadly described.

In summary, anti-TTR variants and conformation-specific antibodies are most commonly used with T24/31, sometimes supplemented by mutation-targeting antibodies and established protein detection techniques.

Key findings from clone T24/31 citations in scientific literature indicate its primary use as a monoclonal antibody for in vivo and ex vivo T cell depletion—especially in mouse models studying tissue-resident memory T cells (TRM) and immune responses.

Essential Context and Findings:

• Clone T24/31 is identified as a pan-anti-Thy1 monoclonal antibody (IgG2b, from Bio X cell) used for depletion of T cells in mice.

• It is widely employed in experiments to distinguish between tissue-resident and circulating T cell populations. In a prominent study, the antibody was used to deplete peripheral T cells in mouse models of allergic airway disease (HDM-exposed mice), thereby enabling researchers to show that lung CD4^+^ TRM, but not circulating T cells, mediate sustained airway hyperresponsiveness.

• When administered, anti-Thy1 T24/31 antibody depleted 75% of lung CD4^+^ T cells and >95% of CD8^+^ T cells. The remaining lung CD4^+^ cells were identified as TRM by their phenotypic markers and protection from intravascular antibody labeling. This allowed researchers to conclude that TRM populations persist and are functionally distinct after antibody-mediated depletion of circulating cells.

• The biological insight gained is that lung CD4^+^ TRM can maintain immune memory and airway hyperresponsiveness independently of recirculating T cells—a key finding for understanding mechanisms of chronic allergic inflammation and potential targets for therapeutic intervention.

Additional Notes:

• The cited literature does not identify T24/31 as a cell line, but specifically as an antibody reagent for T cell depletion. This clarifies potential ambiguity with T24—a well-known bladder carcinoma cell line—or T24M, its metastatic derivative, but clone T24/31 refers specifically to an antibody used in immunological experimental contexts.

• There are no indications in the search results or the broader literature of clone T24/31 being used outside the context of murine T cell depletion experiments.

Summary Table:| Clone T24/31 | Key Use | Findings Supported ||--------------|---------------------|------------------------------------------------------------|| Anti-Thy1 Ab | T cell depletion in mice | Permits in situ study of TRM cells by depleting circulating T cells |

If you are seeking findings about T24 as a cell line or gene clone, please clarify your intent, but the above represents the literature profile for clone T24/31 as cited in immunological research.