Anti-Mouse CD90 (Thy-1) (Clone T24/31) – Purified in vivo PLATINUM™ Functional Grade

Clone T24/31 is a rat monoclonal antibody that recognizes mouse CD90 (Thy-1), a 25-35 kDa GPI-anchored protein expressed on thymocytes, peripheral T cells, and certain intraepithelial T lymphocytes. This antibody has become a valuable tool in immunological research, particularly for manipulating T cell populations in mouse models.

T Cell Depletion Studies

The primary in vivo application of clone T24/31 is T cell depletion in mouse models. The antibody recognizes a non-polymorphic determinant on both Thy1.1 and Thy1.2 alloantigens, allowing it to target T cells across different mouse strains. This makes it particularly useful for studying immune responses in the absence of T cells or for examining the role of specific T cell populations in various physiological and pathological conditions.

Tissue-Resident Memory T Cell Research

Clone T24/31 has been specifically utilized in studies investigating tissue-resident memory T cells (TRM). These specialized T cell populations remain in peripheral tissues and provide localized immune surveillance. By depleting T cells with this antibody, researchers can examine how TRM populations develop, maintain themselves, and contribute to immune protection in different tissue compartments.

Ex Vivo T Cell Priming Assays

Beyond direct in vivo depletion, clone T24/31 is employed in ex vivo T cell priming protocols where it helps enrich T cell populations from mouse spleens and lymph nodes for subsequent functional studies. In these applications, the antibody facilitates the isolation and manipulation of T cells that will be used to study dendritic cell-T cell interactions and antigen presentation mechanisms.

The antibody is manufactured in ultra-low endotoxin formulations (typically <0.5 EU/mg) with high purity (>95% by SDS-PAGE), making it suitable for in vivo functional studies where endotoxin contamination could confound experimental results.

The T24/31 antibody, which targets mouse CD90 (also known as Thy-1), is commonly used in immunological studies, particularly for T cell depletion in mouse models. While specific antibodies used alongside T24/31 in the literature are not detailed in the provided search results, here are some general applications and companion proteins that might be used in similar contexts:

1. Beta Tubulin: In immunocytochemistry, T24/31 is often paired with antibodies like beta tubulin for dual labeling, which helps in identifying specific cell types or structures.

2. CD200: When studying immune responses, antibodies targeting CD200, such as clone OX-90, might be used in conjunction with T24/31 for more comprehensive analysis of immune cell populations.

3. General Companion Proteins in Immunology: Other proteins and antibodies used in similar studies include those targeting various immune cell markers (e.g., CD4, CD8, CD11b), which are crucial for characterizing immune responses and cell populations.

These examples illustrate the kinds of antibodies or proteins that might be used in conjunction with T24/31 in research settings, although specific pairings can vary based on the research question and experimental design.

The key findings from scientific literature citations on clone T24/31 focus on its role as a monoclonal antibody for in vivo and ex vivo T cell depletion in mouse models, especially in studies of tissue-resident memory T cells (TRM) and broader immune responses.

Supporting Details:

• Clone T24/31 is a pan-anti-Thy1 (CD90) monoclonal antibody (IgG2b isotype) used to target the Thy-1 antigen, which is broadly expressed on mouse T cells.
• Its main application in scientific research is for functionally depleting T cells in mice—a critical tool in experiments dissecting the roles of T cells in immunity, inflammation, and tissue-specific immune memory.
• It helps define the functional roles of different T cell subsets, such as tissue-resident memory T cells, by allowing selective removal of these populations in live animals or in cell cultures.
• The antibody is also used in flow cytometry and immunofluorescence applications, sometimes supporting studies on neurite outgrowth in neuronal biology, when applied to certain neuronal cultures. In these contexts, the antibody has been demonstrated to promote neurite outgrowth in peripherin-stained sympathetic rat neurons.

In summary:

• The primary scientific usage of clone T24/31 is as a depleting antibody to study T cell function and memory, particularly in mouse immune system modeling.
• No direct evidence indicates that T24/31 is used for studies involving human T24 cells (e.g., the T24 bladder carcinoma line); its applications are specific to mouse immunology and cell biology.

If you require findings related to the T24 human bladder carcinoma cell line instead (as sometimes “T24” may refer to this context), please clarify your interest.

Dosing regimens of clone T24/31 (anti-mouse CD90/Thy-1) in mouse models vary considerably depending on several experimental factors, including the type of mouse model, stage of tumor establishment, the compound or therapy being tested in combination, and the specific objectives of the study.

Key details supported by current search results:

• Variation by Experimental Objective: Investigators adjust dosing protocols of clone T24/31 based on their specific research goals (such as immune cell depletion, flow cytometry, or functional assays) and model parameters like tumor growth phase or disease state.
• Determined by Application: For cell staining, a recommended dose is up to 2.0 μg per (10^6) cells in 100 μl volume, but titration is advised for each unique experiment. For in vivo or depletion applications, optimal dosage and frequency are not fixed and require individual titration by the experimenter.
• Mouse Model Context: The regimen may further depend on the genetic background of the mice, presence or absence of tumors, or use in specific disease models (e.g., chemically induced inflammation, as in DSS models).
• No Universally Fixed Regimen: Product instructions and published protocols advise that each investigator must empirically determine the most effective dosing schedule and concentration for their particular model system and application.

There is no single standardized protocol for dosing clone T24/31 across all mouse models; regimens are tailored case-by-case, with guidance recommending titration and optimization for the unique experimental context. If your application is cell depletion, tumor immunology, or tissue-specific targeting, dose, administration schedule, and even route (i.e., intraperitoneal, intravenous) may differ, so consulting primary literature for model-specific regimens or performing pilot titration studies is essential.