Anti-Mouse CTLA-4 (CD152) [Clone UC10-4F10-11] — Purified in vivo PLATINUM™ Functional Grade

The clone UC10-4F10-11 is used in in vivo mouse studies primarily to neutralize murine CTLA-4 (CD152), thereby blocking the inhibitory effects of CTLA-4 on T cell activation and promoting T cell co-stimulation in immunological experiments.

In detail:

• UC10-4F10-11 is an Armenian hamster IgG monoclonal antibody.
• In in vivo applications, it is injected into mice to block CTLA-4 binding to its B7 co-receptors, preventing negative regulation of T cells and thus allowing for enhanced CD28-mediated T cell co-stimulation.
• This blockade augments the immune response, often in studies investigating immune checkpoint blockade therapies, tumor immunity, or fundamental T cell costimulation biology.
• Beyond in vivo use, UC10-4F10-11 is also validated for flow cytometry and Western blot detection of mouse CTLA-4, but its unique value is for live animal studies where functional blockade of CTLA-4 is required.

While other anti-CTLA-4 clones (like 9H10 and 9D9) can also deplete intra-tumoral regulatory T cells (Tregs), UC10-4F10-11 is primarily characterized as a CTLA-4 neutralizing antibody rather than a depleting one. It is recommended as a robust tool for studying immune checkpoint blockade and can be obtained in low-endotoxin, in vivo-ready formulations for research purposes.

The correct storage temperature for the sterile packaged clone UC10-4F10-11 (anti-mouse CTLA-4/CD152 antibody) is 2–8°C (refrigerated) for short-term storage (up to one month). For longer-term storage, it should be aliquoted and stored at ? -70°C.

• The antibody is typically shipped with an ice pack, and on receipt, it should be stored at the recommended temperature immediately.
• Some formulations (such as conjugated antibodies in solution) specify to store undiluted at 4°C and not to freeze.
• To avoid loss of activity, repeated freeze/thaw cycles should be avoided.
• Always check the product-specific datasheet for any variation due to formulation or conjugate.

If your UC10-4F10-11 is a purified, functional grade antibody, use 2–8°C for up to a month, and aliquot at ? -70°C for longer storage. If the antibody is conjugated (e.g., PE or APC), store undiluted at 4°C and do not freeze.

When using UC10-4F10-11 (an anti-mouse CTLA-4 antibody) in research, other commonly used antibodies or proteins include:

• Anti-mouse CTLA-4 antibodies from other clones: 9H10 (Syrian hamster IgG) and 9D9 (mouse IgG2b) are frequently cited alternatives or comparators in literature, with overlapping but distinct mechanisms—especially regarding Treg depletion and neutralization.
• Isotype controls: Armenian hamster IgG isotype controls are typically employed as negative controls during in vivo studies to account for non-specific binding effects.
• CD28 antibodies or proteins: Since CTLA-4 and CD28 have competing roles for the B7 ligands, researchers sometimes use anti-CD28 antibodies to study T cell co-stimulation alongside CTLA-4 blockade.
• Other T cell markers and checkpoint antibodies: Studies commonly utilize antibodies against CD3, CD4, CD8, FoxP3 (for Tregs), and sometimes anti-PD-1 or anti-PD-L1 in combination immunotherapy experiments to profile immune cell populations and checkpoint interplay.
• Secondary detection reagents: For flow cytometry or immunoblot, labeled secondary antibodies targeting Armenian hamster IgG (the isotype for UC10-4F10-11) are used.

In summary, the most commonly referenced antibodies or proteins used with UC10-4F10-11 include other anti-CTLA-4 clones (9H10, 9D9), isotype controls, anti-CD28, and various T cell phenotyping antibodies. Experimental context (e.g., tumor immunity, T cell studies) determines which are prioritized.

Clone UC10-4F10-11 is a widely used monoclonal antibody targeting murine CTLA-4 (CD152), with several key findings and applications documented in the scientific literature:

• Neutralization and Detection: UC10-4F10-11 is an Armenian hamster IgG that neutralizes murine CTLA-4 both in vitro and in vivo. It is effective for Western blot and uniquely validated for flow cytometry—differentiating it in application versatility from other anti-CTLA-4 clones.

• Functional Mechanism: This antibody promotes T cell co-stimulation by blocking CTLA-4’s binding to B7 ligands (CD80/CD86), thus permitting costimulatory signaling via CD28, which enhances T cell activation.

• Immunomodulatory Effects in Disease Models: Injection of UC10-4F10-11 during the priming phase of immune responses has been shown to:

  • Inhibit Th1 memory responses and confer protection in experimental models such as TNBS-induced colitis.
  • Induce the development of CD4^+^ ICOS^high^ T cells that produce higher levels of IL-10 and IL-4, with a concomitant reduction in IFN?, suggesting a shift toward regulatory/Th2-like immune profiles.
  • This effect is dependent on STAT6, implicating Th2 polarization in the skewing of the immune response after anti-CTLA-4 treatment.

• Experimental Applications:

  • UC10-4F10-11 is employed for blocking assays, flow cytometric analysis of CTLA-4 expression, and functional studies of T cell activation and regulation in murine models.
  • The antibody is produced to high purity standards and is commonly used as a reference clone for assessing CTLA-4 function and immune modulation.

In summary, scientific literature cites clone UC10-4F10-11 as a reliable tool for both mechanistic studies of CTLA-4 in mouse models and for inducing regulatory or Th2-biased immune responses, especially during experimental manipulations of immune priming and disease models.