Anti-Mouse CTLA-4 (CD152) [Clone UC10-4F10-11] — Purified in vivo PLATINUM™ Functional Grade
Anti-Mouse CTLA-4 (CD152) [Clone UC10-4F10-11] — Purified in vivo PLATINUM™ Functional Grade
Product No.: C2444
Clone UC10-4F10-11 Target CTLA-4 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names CD152, Cytotoxic T Lymphocyte-Associated Antigen-4, Ly-56 Isotype IgG1 Applications FA , FC , in vivo , IP , WB |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Armenian Hamster Recommended Isotype Controls Recommended Dilution Buffer Immunogen Mouse CTLA-4 IgG2a Fusion Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2829541 Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone UC10-4F10-11 recognizes an epitope on mouse CTLA-4. Background CTLA-4 is a 33 kD member of the Ig superfamily similar to CD28 in amino acid sequence, structure, and genomic organization. CTLA-4 is a protein receptor that functions as an immune checkpoint and downregulates immune responses. It is involved in the development of protective immunity and thymocyte regulation, in addition to the induction and maintenance of immunological tolerance. CTLA-4 has therapeutic potential both as an agonist to reduce immune activity, and an antagonist to increase immune activity. Antigen Distribution CTLA-4 is expressed on activated T and B lymphocytes. Ligand/Receptor CD80 (B7-1), CD86 (B7-2) NCBI Gene Bank ID UniProt.org Research Area Immunology Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone UC10-4F10-11 is widely used in in vivo mouse studies to neutralize murine CTLA-4 (CD152), thereby blocking CTLA-4-mediated inhibition of T cell activation and promoting immune responses, most frequently in immunology and immuno-oncology research. Key in vivo applications of clone UC10-4F10-11 in mice include:
Additional applications (sometimes overlapping with in vitro use) include flow cytometry detection of mouse CTLA-4 on activated T cells, and validation in functional assays demonstrating T cell activation. In summary, UC10-4F10-11 is a standard tool for in vivo blockade of CTLA-4 in mice to model and dissect T cell-mediated immune activation and checkpoint inhibition in a variety of disease contexts. Commonly used antibodies or proteins paired with UC10-4F10-11 (an anti-mouse CTLA-4 clone) in the literature include a range of T cell, immune checkpoint, and phenotyping antibodies, as well as isotype and experimental controls. The most frequently co-used antibodies and proteins are:
The specific combination depends on the experiment, such as tumor immunity studies (using broader immune phenotyping and checkpoint controls), or T cell functional assays focusing on co-inhibitory/co-stimulatory signaling pathways. Key findings from scientific literature on clone UC10-4F10-11 (an anti-mouse CTLA-4 monoclonal antibody) can be summarized as follows:
In summary, UC10-4F10-11 is a standard tool for blocking CTLA-4 in mouse immunological studies, enabling researchers to dissect T cell co-stimulation, immune checkpoint function, and antitumor immunity in a neutralizing, non-Treg-depleting fashion. Dosing regimens for clone UC10-4F10-11 (anti-mouse CTLA-4) in mouse models typically fall within a range of 50–250 µg per mouse per dose, but exact regimens are highly dependent on mouse strain, experimental context, and disease model. Commonly published protocols use 100 µg per mouse administered intraperitoneally (i.p.) at multiple discrete time points, while some preclinical studies use higher doses adjusted by weight, such as 20 mg/kg. Key dosing regimens across different models:
Additional considerations:
Summary Table:| Mouse Model / Experimental Context | Typical Dose and Schedule ||----------------------------------------|------------------------------------------------|| General in vivo (various strains) | 50–250 µg/mouse per dose || C57BL/6, B16 melanoma (tumor) | 100 µg/mouse i.p. on days 4, 7, and 10 || Tumor models (weight-based) | 20 mg/kg (≈400 µg for 20g mouse) || Immunization/autoimmune priming | 100 µg/mouse on 3 consecutive days | In summary, regimens using clone UC10-4F10-11 are generally consistent in dose (100–250 µg/mouse), but can vary by timing, frequency, and weight adjustments depending on the specific mouse strain and experimental application. References & CitationsTechnical ProtocolsCertificate of Analysis |
Formats Available
