Anti-Mouse Dendritic Cells – Biotin

Home/Primary Monoclonal Antibodies/Surfacetag Mouse CD Markers/Anti-Mouse Dendritic Cells – Biotin

Pricing & Details

Product No.D105
Clone
33D1
Protein
Dendritic Cells
Formats AvailableView All
Product Type
Monoclonal Antibody
Alternate Names
DC Marker, 33D1, DCIR2 (dendritic cell inhibitory receptor 2)
Isotype
Rat
IgG2b
Applications
FC
Prod No.
Size
Price
Avail.
Qty
Add to cart
D105-100 µg
100 µg
$77.00
In stock
Max:
Min: 1
Step: 1
D105-500 µg
500 µg
$253.00
In stock
Max:
Min: 1
Step: 1

Antibody Details

Product Details

Reactivity Species
Mouse
Host Species
Rat
Product Concentration
0.5 mg/ml
Formulation
This biotinylated antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This biotinylated antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day Ambient
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Clone 33D1 recognizes mouse DCIR2.
Antigen Distribution
Murine DCIR2 is found on dendritic cells of the thymus, spleen, lymph nodes, and Peyer’s patches. DCs in the bone marrow may express DCIR2 in the presence of GM-CSF. However, this expression is notably downregulated when IL-4 is present. Furthermore, DCIR2 has been found In vivo on brain dendritic cells post infection with T. gondii.
Background
Dendritic cells are antigen presenting cells that have two functions. They scan the body collecting and processing antigen material that they present on the cell surface to T cells, and they maintain T cell tolerance to “self”. The morphology of dendritic cells is characterized by an extremely large surface-to-volume ratio. Murine splenic dendritic cells can occur in two types: myeloid (cDC) and lymphoid (pDC). Lymphoid dendritic cells produce high amounts of IFN-α and are also called Plasmacytoid dendritic cell because they have an appearance similar to plasma cells. Myeloid, or conventional dendritic cells, secrete IL-12, IL-6, TNF, and chemokines and can be further categorized into three subtypes (CD4−CD8+, CD4+CD8− and CD4−CD8−). These differ from other migratory dendritic cells such as Langerhans cells and interstitial dendritic cells that migrate from peripheral tissues to the lymph nodes. The exact nature and biological activity of the dendritic cell surface marker DCIR2 is currently unknown. DCs are known to play a role in several diseases including myeloid cancer, pDC leukemia, HIV, lupus erythematosus, Crohn's disease and ulcerative colitis. However, it is thought that DCs may be able to control cancer progression because increased densities of DC populations have been linked with better clinical outcome. Lung cancers have been found to include four different subsets of dendritic cells; some of which can activate immune cells that can suppress tumor growth. Dendritic cells have also been shown to play a role in the success of cancer immunotherapies in experimental models. Specifically, the immune checkpoint blocker anti-PD-1 has been shown to indirectly activate DCs through IFN-γ released from drug-activated T cells. Agonizing the non-canonical NF-κB pathway also activates DCs and further enhances anti-PD-1 therapy in an IL-12-dependent manner.

Antigen Details

Research Area
Immunology

References & Citations

Steinman, R. M. et al. (1982) Pro. Natl. Acad. Sci. USA 79:161 Steinman, R. M. et al. (1983) J. Exp. Med. 157:613
Flow Cytometry

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.