Anti-Mouse F4/80 – Purified in vivo GOLD™ Functional Grade

Thioglycolate-induced peritoneal macrophages from C57BL/6 mice

This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.

Liquid

Functional grade preclinical antibodies are manufactured in an animal free facility using only in vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.

Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.

Research Use Only

2 – 8° C Wet Ice

Cl:A3-1 activity is directed against mouse F4/80.

Macrophages play an important role in innate and adaptive immune system functions¹. F4/80 antigen is a widely used cell-surface marker of murine macrophages^{1, 2} encoded by the Adgre1 locus³. F4/80 antigen is a G protein-coupled receptor with an extracellular domain containing repeated Epidermal Growth Factor-like calcium-binding domains and as such is a member of the EGF-TM7 protein family, which itself is a subfamily of the adhesion G protein-coupled receptors (ADGRE) family. F4/80 is thought to have dual adhesion and signaling functions due to its receptor domains and is known to be required for the differentiation of antigen-specific CD8⁺ T regulatory cells¹ . Adgre1 null mutant mice have a dysregulated autoimmune response and regulatory T cell generation³. Additionally, unstimulated resting macrophages express F4/80 at higher levels than activated macrophages¹.

Cl:A3-1 was generated by immunizing a rat with thioglycolate-induced peritoneal macrophages from C57BL/6 mice⁴. Spleen cells were fused with mouse myeloma cell line NS1. Cl:A3-1 does not bind to macrophages via Fc receptors and is not cytotoxic. Cl:A3-1 is able to modulate Listeria-induced cytokine signaling between macrophages and natural killer cells².

F4/80 is constitutively expressed on most macrophages, except those located in T cell areas and marginal zones of lymph nodes and spleen, as well as on some dendritic cells. Blood monocytes also express F4/80, but at low levels relative to mature macrophages.

N/A

1 Lin HH, Faunce DE, Stacey M, et al. J Exp Med. 201(10):1615-1625. 2005.
2 Warschkau H, Kiderlen AF. J Immunol. 163(6):3409-16. 1999.
3 Waddell LA, Lefevre L, Bush SJ, et al. Front Immunol. 9:2246. 2018.
4 Austyn JM, Gordon S. Eur J Immunol. 11(10):805-815. 1981.
5 Hume DA, Perry VH, Gordon S. Anat Rec. 210(3):503-512. 1984.
6 Perry VH, Hume DA, Gordon S. Neuroscience. 15(2):313-326. 1985.
7 Morris L, Graham CF, Gordon S. Development. 112(2):517-526. 1991.
8 Haidl ID, Jefferies WA. Eur J Immunol. 26(5):1139-1146. 1996.