Anti-Mouse IL-12 p70
Anti-Mouse IL-12 p70
Product No.: I-666
- -
- -
Clone 48110 Target IL-12 p70 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names NKSF, TSF, Maturation Factor, Cytotoxic Lymphocyte Maturation Factor (CLMF), p35, Interleukin-12 p70 Isotype IgG1 Applications ELISA Cap , ELISPOT , WB |
- -
- -
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Immunogen Purified Recombinant Mouse IL-12 p70 (>98%) Purity >95% by SDS Page and HPLC Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2830787 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is at a concentration of approximately 1.0-0.4 µg/ml. ELISPOT Capture: This Low Endotoxin Functional Formulation antibody has been reported for use as the capture antibody in a sandwich ELISPOT assay. Aseptically coat plates at a concentration of 2-8 µg/ml. A suitable detection antibody is PN:I-268. Western Blotting: To detect Mouse IL-12 p70 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse IL-12 p70 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is I-219. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rat Anti-Mouse Interleukin-12 p70 (IL-12 p70) (Clone 48110) recognizes an epitope on Mouse IL-12 p70. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody detects an epitope within the p35 subunit. Background Interleukin 12 (IL-12, NK cell stimulatory factor, cytotoxic lymphocyte maturation factor) is a heterodimeric cytokine that is naturally produced by dendritic cells1 , macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 is involved in the differentiation of naive T cells into Th1 cells, which is important in resistance against pathogens. It is known as a T cell stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of IFN-γ and TNF-α from T and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-γ. IL-12 also has anti-angiogenic activity, which can block the formation of new blood vessels. IL-12 binds to the IL-12 receptor and upon binding, IL-12R-β2 becomes tyrosine phosphorylated and provides binding sites for kinases, Tyk2 and Jak2. These are important in activating critical transcription factor proteins such as STAT4 which are implicated in IL-12 signaling in T cells and NK cells.2 IL-12 contributes to the antimycobacterial immune response by enhancing production of interferon-gamma, facilitating development of Th1 cells and augmenting cytotoxicity of antigen-specific T cells and natural killer cells.3 Antigen DetailsPubMed Research Area Other Molecules References & Citations1. Kapsenberg, ML. et al. (1997) J. Immunol. 159: 28 2. Ritz, J. et al. (2001) Blood. 97(12):3860-6. 3. Barnes, PF. et al. (1994) J Clin Invest. 93(4):1733-9. Technical Protocols ELISPOT  |
Related Products
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
