Anti-Mouse IL-12 p70
Anti-Mouse IL-12 p70
Product No.: I-666
Formats AvailableView All
NKSF, TSF, Maturation Factor, Cytotoxic Lymphocyte Maturation Factor (CLMF), p35, Interleukin-12 p70
Purified Recombinant Mouse IL-12 p70 (>98%)
>95% by SDS Page and HPLC
This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.3 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present.
Storage and Handling
The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months.
Country of Origin
Next Day Ambient
Applications and Recommended Usage?
Quality Tested by Leinco
ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 2-8 µg/ml. A suitable detection antibody is at a concentration of approximately 1.0-0.4 µg/ml.
ELISPOT Capture: This Low Endotoxin Functional Formulation antibody has been reported for use as the capture antibody in a sandwich ELISPOT assay. Aseptically coat plates at a concentration of 2-8 µg/ml. A suitable detection antibody is PN:I-268.
Western Blotting: To detect Mouse IL-12 p70 this monoclonal antibody can be used at a concentration of 1-2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T343. The detection limit for Mouse IL-12 p70 is 25 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is I-219.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Rat Anti-Mouse Interleukin-12 p70 (IL-12 p70) (Clone 48110) recognizes an epitope on Mouse IL-12 p70. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. This antibody detects an epitope within the p35 subunit.
Interleukin 12 (IL-12, NK cell stimulatory factor, cytotoxic lymphocyte maturation factor) is a heterodimeric cytokine that is naturally produced by dendritic cells1 , macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation. IL-12 is involved in the differentiation of naive T cells into Th1 cells, which is important in resistance against pathogens. It is known as a T cell stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of IFN-γ and TNF-α from T and natural killer (NK) cells, and reduces IL-4 mediated suppression of IFN-γ. IL-12 also has anti-angiogenic activity, which can block the formation of new blood vessels. IL-12 binds to the IL-12 receptor and upon binding, IL-12R-β2 becomes tyrosine phosphorylated and provides binding sites for kinases, Tyk2 and Jak2. These are important in activating critical transcription factor proteins such as STAT4 which are implicated in IL-12 signaling in T cells and NK cells.2 IL-12 contributes to the antimycobacterial immune response by enhancing production of interferon-gamma, facilitating development of Th1 cells and augmenting cytotoxicity of antigen-specific T cells and natural killer cells.3
References & Citations
1. Kapsenberg, ML. et al. (1997) J. Immunol. 159: 28
2. Ritz, J. et al. (2001) Blood. 97(12):3860-6.
3. Barnes, PF. et al. (1994) J Clin Invest. 93(4):1733-9.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.