Anti-Murine Norovirus Capsid [Clone A6.2] — Purified in vivo PLATINUM™ Functional Grade

Based on the search results, there are references to clone A6, A6/2/2, and A6.1 in the context of monoclonal antibodies (e.g., anti-AP2, anti-TSP/thrombospondin), but none directly describe an in vivo mouse study specifically using "clone A6.2" as an experimental tool, therapeutic agent, or genetic reagent.

Details from Search Results

• A6/2/2: This is a clone name for a monoclonal anti-AP2 antibody, used for detecting AP-2 (TFAP2) in techniques like immunohistochemistry and Western blot. There is no evidence in the provided sources that this clone is used directly in live mouse experiments (in vivo); instead, it is used for ex vivo or in vitro protein detection.
• A6 (in the context of guide RNA): In a gene-editing study, "A6" refers to a specific single-guide RNA (sgRNA-A6) targeting a site in the Rpe65 gene, used in combination with an adenine base editor (NG-ABE) to correct mutations in a mouse model of Leber congenital amaurosis (LCA). The combination of sgRNA-A6 and NG-ABE was delivered via lentiviral vector injected subretinally, resulting in targeted base editing in retinal pigment epithelium (RPE) cells in vivo. However, this "A6" is a genetic targeting reagent, not an antibody or cell line clone.
• A6.1: This is an anti-thrombospondin monoclonal antibody clone, referenced in a study for immunohistochemistry on mouse tissue sections. Again, this is an ex vivo detection method, not an in vivo application.

Summary Table

Conclusion

There is no evidence in the provided search results that "clone A6.2" is used in in vivo mouse studies. The closest relevant in vivo use is the sgRNA-A6 in a gene-editing context, but this is not an antibody or cell line clone. For monoclonal antibodies with similar names (e.g., A6/2/2, A6.1), their use is limited to ex vivo protein detection, not live animal experiments. If "clone A6.2" refers to a specific cell line, antibody, or reagent not covered in these results, additional information or context would be needed to clarify its in vivo application.

The correct storage temperature for a sterile packaged clone A6.2—assuming it is a biological sample such as cells or antibodies—should generally be below ?80°C for long-term preservation, ideally in liquid nitrogen (LN?, typically at ?150°C to ?190°C).

• For short-term storage (less than 7 days), ?80°C is sufficient.
• For long-term storage and best preservation of function, transfer the sample to liquid nitrogen storage—either the vapor or liquid phase—to ensure molecular activity is completely halted and cell viability/functionality are sustained.

Supporting Details:

• Storage in liquid nitrogen (either vapor or liquid phase) is advised for biological samples like PBMCs, stem cells, or clones to avoid metabolic activity and degradation, as molecular movement continues even at ?80°C but halts at the water glass transition (~?130°C).
• Handling should minimize exposure to elevated temperatures, especially during transfers (use dry ice for brief transitions, but not for storage).
• In contrast, sterile-packaged non-cellular medical devices or supplies may be stored at 18–23°C, but this does not apply to living biological samples.

If “clone A6.2” refers to a specific cell line or antibody preparation, follow manufacturer guidelines if supplied—otherwise, the above protocol applies for biological sample integrity. If additional details about “clone A6.2” are provided, storage recommendations may be refined.

Commonly Used Antibodies and Proteins with "A6.2" in Scientific Literature

The search results do not explicitly detail antibodies or proteins commonly used in combination with "A6.2." However, based on the available information, here’s a structured summary of relevant findings and contextual clues:

Antibody and Protein Context for "A6.2"

• AP2 alpha Antibody (A6/2/2): This monoclonal antibody (clone A6/2/2, human reactivity, mouse IgG isotype) is directed against the C-terminal peptide of AP2 alpha. It is validated for various applications such as ELISA, Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Radioimmunoassay, and Western Blot.
• Applications and Controls: The product datasheet notes "matched isotype control" as a standard control in flow cytometry experiments. Positive controls mentioned include Raji cells and breast carcinomas. CyTOF (mass cytometry) readiness is also highlighted, suggesting compatibility with multiplex antibody panels used in advanced cytometry, though no specific companion antibodies are listed.
• Reactive Proteins: The main target is AP2 alpha (and alpha/beta forms), but no direct mention is made of companion antibodies or proteins used synchronously in the literature for this clone.

Comparison to Similar Naming Conventions (A6.x)

• A6.1 Antibody: The literature discusses monoclonal antibody A6.1, generated against thrombospondin-1 (TSP-1), not a companion for A6/2/2, but rather an example of antibodies with a similar naming scheme (A6.x). Epitope mapping studies for A6.1 analyze its binding to TSP-1 fragments and calcium-dependent epitopes. There is no indication that A6.1 is used together with A6/2/2.
• A6 Antibody (CD45/LCA): Another “A6” (not A6.2) recognizes unique isoforms of the leukocyte common antigen (CD45) and is used to identify functional T-cell subsets. Again, this is unrelated to the A6/2/2 clone and is more relevant in immunology and cell phenotyping.

General Practices (Extrapolation)

• Multiplex Analysis: In advanced cytometry (e.g., CyTOF, flow cytometry), it is common to use panels of antibodies targeting different cell markers to simultaneously assess multiple parameters. However, the specific antibodies paired in such panels would depend on the experimental design (e.g., cell type, pathway of interest), not on the A6/2/2 clone itself.
• Positive/Negative Controls: Isotype-matched antibodies and known positive controls (e.g., Raji cells for B-cell markers, breast carcinomas for epithelial markers) are standard in immunodetection, but these are not unique to A6/2/2.
• Gene/Protein Families: If A6/2/2 targets AP2 family members (alpha, beta), one might find literature on other AP2 isoforms (gamma, delta) studied with tools targeting this protein family, but not necessarily specifically with A6/2/2.

Summary Table: Relevant Antibodies and Proteins

Conclusion

There is no direct evidence in the provided literature or product information that specific antibodies or proteins are consistently used together with the A6/2/2 clone in published protocols. The use of isotype controls and known positive control cell lines (e.g., Raji, breast carcinoma) is standard, but not unique to A6/2/2. Companion antibodies in multiplex assays would be experiment-specific. If you have a particular application or biological context, a tailored literature search in that field may yield more specific information on commonly co-used reagents.

I don't have specific information about clone A6.2 citations in the provided search results. The search results contain general information about cloning methodologies, patent citations, and some specific clones, but none specifically mention clone A6.2 or its citation patterns in scientific literature.

The search results do discuss various aspects of cloning research, including synthetic clone pair dataset generation, clonal selection algorithms, and specific cloning vectors like pGEM-T, but they don't contain data about the particular clone A6.2 that you're asking about.

To provide accurate information about the key findings from clone A6.2 citations, I would need search results that specifically reference this clone and its impact in the scientific literature, including citation metrics, research applications, and any notable discoveries or developments associated with it.

If you have access to scientific databases like PubMed, Web of Science, or Google Scholar, searching directly for "clone A6.2" would likely yield more relevant and specific information about its citations and research impact.