Anti-Phosphotyrosine Purified

Pricing & Details

Product No.P111
Clone
PY20
Protein
Antibody
Formats AvailableView All
Product Type
Monoclonal Antibody
Isotype
Mouse
IgG2b
Applications
WB
Prod No.
Size
Price
Avail.
Qty
Add to cart
P111-0.5 mg
0.5 mg
$217.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Antibody Details

Product Details

Reactivity Species
Phosphotyrosine
Host Species
Mouse
Immunogen
KLH-conjugated phosphotyrosine
Product Concentration
0.5 mg/ml
Formulation
This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative.
Storage and Handling
This purified antibody is stable when stored at 2-8°C. Do not freeze.
Country of Origin
USA
Shipping
Next Day 2-8°C
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Anti-Phosphotyrosine (Clone PY20) recognizes phosphorylated tyrosine residues in all species tested (human, mouse, rat, dog, chicken, and frog). This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. The PY20 antibody has been shown to be useful for flow cytometry, immunoprecipitation, WB, and IF.
Antigen Distribution
Phospho-Specific
Background
Tyrosine phosphorylation represents a major mechanism for the activation of signal transduction pathways in response to growth factors, hormones and cytokines. There are approximately 100 tyrosine kinases that are both receptor tyrsosine kinase receptors for various growth factors and cytokines. The extent and duration of tyrosine phosphorylation are tightly regulated by the 107 protein tyrosine phosphatases. Phosphorylation can occur on threonine, serine, and tyrosine residues.

Antigen Details

Protein
PubMed
Research Area
Cell Biology
.
Immunology
.
Signal Transduction

References & Citations

1. Koland, JG. et al. (1998) Biochem J. 334(Pt 1):189-95. Article Link
2. Häring, HU. et al. (1998) Diabetologia 41:833-838. Article Link
3. Katada, T. et al. (1996) J Biol Chem. 271(3):1534-7. Article Link
4. Häring, HU. et al. (1997) Diabetologia. 40(11):1358-62. Article Link
5. Häring, HU. et al. (1997) Diabetologia. 40: 863–866. Article Link
General Western Blot Protocol

Formats Available

Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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