Antibody DetailsProduct DetailsReactivity Species Phosphotyrosine Host Species Mouse Immunogen KLH-conjugated phosphotyrosine Product Concentration 0.5 mg/ml Formulation This purified antibody is formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.4, 1% BSA and 0.09% sodium azide as a preservative. Storage and Handling This purified antibody is stable when stored at 2-8°C. Do not freeze. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2831511 Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Anti-Phosphotyrosine (Clone PY20) recognizes phosphorylated tyrosine residues in all species tested (human, mouse, rat, dog, chicken, and frog). This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. The PY20 antibody has been shown to be useful for flow cytometry, immunoprecipitation, WB, and IF. Antigen Distribution Phospho-Specific Background Tyrosine phosphorylation represents a major mechanism for the activation of signal transduction pathways in response to growth factors, hormones and cytokines. There are approximately 100 tyrosine kinases that are both receptor tyrsosine kinase receptors for various growth factors and cytokines. The extent and duration of tyrosine phosphorylation are tightly regulated by the 107 protein tyrosine phosphatases. Phosphorylation can occur on threonine, serine, and tyrosine residues. Antigen DetailsProtein PubMed Research Area Cell Biology . Immunology . Signal Transduction References & Citations1. Koland, JG. et al. (1998) Biochem J. 334(Pt 1):189-95. Article Link 2. Häring, HU. et al. (1998) Diabetologia 41:833-838. Article Link 3. Katada, T. et al. (1996) J Biol Chem. 271(3):1534-7. Article Link 4. Häring, HU. et al. (1997) Diabetologia. 40(11):1358-62. Article Link 5. Häring, HU. et al. (1997) Diabetologia. 40: 863–866. Article Link Technical Protocols |
Formats Available
Prod No. | Description |
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P347 |
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P339 |
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P111 |
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P112 |
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P113 |
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P114 |
