Anti-Rel (Phospho-Ser503) Antibody (43020)

Anti-Rel (Phospho-Ser503) Antibody (43020)

Product No.: 43020

- -
- -
Target
Rel (Phospho-Ser503)
Product Type
Polyclonal
Isotype
Rabbit
Applications
IF

- -
- -
Select Product Size

Data

Immunofluorescence: Detection of Rel (phospho-503) in methanol-fixed HeLa cells.
- -
- -

Antibody Details

Product Details

Reactivity Species
Human
Host Species
Rabbit
Immunogen
Peptide sequence that includes phosphorylation site of Serine 503 (T-S-S(p)-D-S) derived from human Rel and conjugated to KLH.
Product Concentration
1.0 mg/ml
Formulation
PBS (without Mg2 and Ca2 ), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
State of Matter
Liquid
Product Preparation
Affinity-purified on phosphopeptide; non-phosphopeptidereactive antibodies were removed by chromatography on non-phosphorylated peptide
Storage and Handling
This antibody is stable for at least one (1) year at -20°C.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco
Immunofluorescence: use at dilution of 1:100-1:200.

These are recommended working dilutions.

End user should determine optimal dilutions for their applications.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Polyclonal Antibody specific to Rel (Phospho-Ser503)
Background
Transcription factor c-Rel is a member of the Rel/NFkappaB family which also includes Rel-A, Rel-B, NFkappaB1, and NFkappaB2. These proteins are related through a highly conserved N-terminal region referred to as the “Rel domain†which is responsible for DNA binding, dimerization, nuclear localization, and binding to the NFkappaB inhibitor.

Antigen Details

Function
Proto-oncogene that may play a role in differentiation and lymphopoiesis. NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. The NF-kappa-B heterodimer RELA/p65-c-Rel is a transcriptional activator.
NCBI Gene Bank ID
UniProt.org
Research Area
Phosphospecific Antibodies

References & Citations

IF
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.