Anti-Replication Factor C (p37) Antibody (56253)
Anti-Replication Factor C (p37) Antibody (56253)
Product No.: 56253
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Clone 1320 Target Replication Factor C (p37) Formats AvailableView All Product Type Monoclonal Alternate Names Activator 1 40 kDa subunit, A1 40 kDa subunit, Activator 1 subunit 2, Replication factor C 40 kDa subunit, RF-C 40 kDa subunit, RFC40 Isotype Mouse IgG2b Applications ICC , IF , WB |
Data
![Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p37 antibody [1320] (56253) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.](https://www.leinco.com/wp-content/uploads/2025/01/qed-bioscience-anti-replication-factor-c-p37-antibody-56253-1.jpg)
Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with p37 antibody [1320] (56253) diluted at 1:500. The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody. antibody at 1:200 dilution.](https://www.leinco.com/wp-content/uploads/2025/01/qed-bioscience-anti-replication-factor-c-p37-antibody-56253-2.jpg)
Immunofluorescence analysis of methanol-fixed MCF7, using p37 [1320](56253) antibody at 1:200 dilution. - -
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Antibody DetailsProduct DetailsReactive Species Human ⋅ Yeast Host Species Mouse Immunogen Recombinant protein corresponding to the p37 subunit of human Replication Factor C expressed in E. coli. Product Concentration Lot Specific Formulation PBS, pH 7.4. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This antibody is stable for at least one (1) year at -70°C. Avoid multiple freeze-thaw cycles. Regulatory Status Research Use Only Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1:2,000 dilution. A band of 37 kD is detected.
Positive controls: HeLa cell lysate. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This antibody recognizes the p37 subunit of human and yeast (S. cerevisiae) Replication Factor C (RFC). RFC is a heteropentameric protein consisting of p140, p40, p38, p37, and p36 subunits, RFC participates in targeting DNA polymerase delta processivity factor (PCNA) on to a primed DNA template during DNA synthesis and repair. RFC has also been associated with DNA repair in conjunction with a PCNA-like heterotrimer of Hus1, Rad1, and Rad9. Function The elongation of primed DNA templates by DNA polymerase delta and epsilon requires the action of the accessory proteins proliferating cell nuclear antigen (PCNA) and activator 1. This subunit binds ATP (By similarity). {ECO:0000250}. NCBI Gene Bank ID UniProt.org Research Area Cancer Research References & CitationsTechnical ProtocolsICC IF  Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
