Anti-SNAT1 Neurotransmitter Transporter Antibody (56522)
Anti-SNAT1 Neurotransmitter Transporter Antibody (56522)
Product No.: 56522
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Clone S104-32 Target SNAT1 Neurotransmitter Transporter Formats AvailableView All Product Type Monoclonal Alternate Names Amino acid transporter A1, rATA1, Glutamine transporter, N-system amino acid transporter 2, Solute carrier family 38 member 1, System A amino acid transporter 1, System A transporter 2, System N amino acid transporter 1 Isotype Mouse IgG1 Applications ICC , IF , IHC , WB |
Data
Western Blot analysis of Rat brain membrane lysate showing detection of SLC38A1 protein using Mouse Anti-SLC38A1 Monoclonal Antibody, Clone S104-32 (56522). Primary Antibody: Mouse Anti-SLC38A1 Monoclonal Antibody (56522) at 1:1000.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SNAT1 Monoclonal Antibody, Clone S104-32 (56522). Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 4% PFA for 15 min. Primary Antibody: Mouse Anti-SNAT1 Monoclonal Antibody (56522) at 1:200 for overnight at 4°C with slow rocking. Secondary Antibody: AlexaFluor 488 at 1:1000 for 1 hour at RT. Counterstain: Phalloidin-iFluor 647 (red) F-Actin stain; Hoechst (blue) nuclear stain at 1:800, 1.6mM for 20 min at RT. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) SNAT1 Antibody (D) Composite.
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-SNAT1 Monoclonal Antibody, Clone S104-32 (56522). Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-SNAT1 Monoclonal Antibody (56522) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60min RT, 5min RT. Localization: Cell Membrane. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) SNAT1 Antibody. (D) Composite. - -
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Antibody DetailsProduct DetailsReactive Species Human ⋅ Mouse ⋅ Rat Host Species Mouse Immunogen Fusion protein corresponding to aa 1-63 (cytoplasmic N-terminus) of rat SNAT1 (accession no. NP_620187.1). Product Concentration 1.0 mg/ml Formulation PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography. State of Matter Liquid Product Preparation Purified by Protein G affinity chromatography Storage and Handling This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles. Regulatory Status For in vitro investigational use only. Not for
use in therapeutic or diagnostic procedures. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Immunoblotting: use at 1-2ug/mL. A band of ~50kDa is detected.
Immunohistochemistry: use at 1-5ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: Rat neocortical neurons cultured under amino acid starvation conditions. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity This antibody recognizes human, mouse,
and rat SNAT1. Background The sodium-coupled neutral amino acid transporters (SNAT) include System A subtypes SNAT1, SNAT2, and SNAT4 and System N subtypes SNAT3 and SNAT5. These transporters are essential for uptake of nutrients, energy production, metabolism, detoxification, and the cycling of neurotransmitters. SNAT1 is responsible for transport of glutamine and may be involved in the generation of glutamate in the retina. SNAT1 is found in heart, brain, and placenta, and expression levels are high in certain neuronal populations in the central nervous system. Function Functions as a sodium-dependent amino acid transporter. Mediates the saturable, pH-sensitive and electrogenic cotransport of glutamine and sodium ions with a stoichiometry of 1:1. May also transport small zwitterionic and aliphatic amino acids with a lower affinity. May supply glutamatergic and GABAergic neurons with glutamine which is required for the synthesis of the neurotransmitters glutamate and GABA. {PubMed:10660562, PubMed:11692272, PubMed:11756489, PubMed:12684517}. NCBI Gene Bank ID UniProt.org Research Area Neuroscience References & CitationsTechnical ProtocolsICC IF   Certificate of Analysis |
Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
