Anti-Tet Repressor Antibody (12594)

Anti-Tet Repressor Antibody (12594)

Product No.: 12594

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Target
Tet Repressor
Product Type
Polyclonal
Alternate Names
Tetracycline Repressor, Tetracycline repressor protein class D
Isotype
Rabbit
Applications
ELISA

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Antibody Details

Product Details

Reactivity Species
Eukaryotic
Host Species
Rabbit
Immunogen
Synthetic peptide corresponding to aa 31-49 (AQKLGVEQPTLYWHVKNKR) of the Tet repressor protein.
Product Concentration
1.0 mg/ml
Formulation
PBS, 1mg/ml BSA, 0.02% sodium azide.
State of Matter
Liquid
Product Preparation
Purified by immunoaffinity chromatography
Storage and Handling
Upon arrival, we recommend aliquoting the undiluted antibody into smaller working volumes (10-30 µL/vial, depending on your typical usage). Store these aliquots long-term at -20°C or -80°C. Keep a separate working aliquot at 4°C for short-term use. To maintain antibody integrity, avoid repeated freeze/thaw cycles. The antibody is stable for at least one year when stored as recommended.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco


Western Blot: use at 1ug/ml, detection limit 0.8ng TetR

ELISA: detection limit 0.2ng TetR.

End users should determine optimal concentrations for their applications. Not all applications have been investigated.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Polyclonal Antibody specific to Tet Repressor
Background
The Tet repressor protein (TetR), initially discovered in Gram-negative bacteria where it plays a crucial role in tetracycline resistance, forms the foundation of the innovative Tet system. This system has emerged as the most widely used and effective method for achieving inducible, conditional gene expression in a vast array of eukaryotic cells. While TetR can, in certain organisms, directly regulate gene expression on its own, its application in more complex systems like mammals and many other eukaryotes typically involves fusing it with specific eukaryotic transcriptional activator or repressor domains. This modification enhances its efficiency and allows for precise, robust control over gene activity.

Antigen Details

UniProt.org
P0ACT4
Research Area
DNA

References & Citations

Indirect Elisa Protocol
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.