Anti-Tet Repressor Antibody (12594)
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Antibody DetailsProduct DetailsReactivity Species Eukaryotic Host Species Rabbit Immunogen Synthetic peptide corresponding to aa 31-49 (AQKLGVEQPTLYWHVKNKR) of the Tet
repressor protein. Product Concentration 1.0 mg/ml Formulation PBS, 1mg/ml BSA, 0.02% sodium azide. State of Matter Liquid Product Preparation Purified by immunoaffinity chromatography Storage and Handling Upon arrival, we recommend aliquoting the undiluted antibody into smaller working volumes (10-30 µL/vial, depending on your typical usage). Store these aliquots long-term at -20°C or -80°C. Keep a separate working aliquot at 4°C for short-term use. To maintain antibody integrity, avoid repeated freeze/thaw cycles. The antibody is stable for at least one year when stored as recommended. Country of Origin USA Shipping Next Day 2-8°C Applications and Recommended Usage? Quality Tested by Leinco Western Blot: use at 1ug/ml, detection limit 0.8ng TetR ELISA: detection limit 0.2ng TetR. End users should determine optimal concentrations for their applications. Not all applications have been investigated. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rabbit Polyclonal Antibody specific to Tet Repressor Background The Tet repressor protein (TetR), initially discovered in Gram-negative bacteria where it plays a crucial role in tetracycline resistance, forms the foundation of the innovative Tet system. This system has emerged as the most widely used and effective method for achieving inducible, conditional gene expression in a vast array of eukaryotic cells. While TetR can, in certain organisms, directly regulate gene expression on its own, its application in more complex systems like mammals and many other eukaryotes typically involves fusing it with specific eukaryotic transcriptional activator or repressor domains. This modification enhances its efficiency and allows for precise, robust control over gene activity. Antigen DetailsUniProt.org P0ACT4 Research Area DNA References & CitationsTechnical Protocols |
