Anti-Tet Repressor Antibody (12594)

Anti-Tet Repressor Antibody (12594)

Product No.: 12594

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Target
Tet Repressor
Product Type
Polyclonal
Isotype
Rabbit
Applications
ELISA

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Antibody Details

Product Details

Reactivity Species
Eukaryotic
Host Species
Rabbit
Immunogen
Synthetic peptide corresponding to aa 31-49 (AQKLGVEQPTLYWHVKNKR) of the Tet repressor protein.
Product Concentration
1.0 mg/ml
Formulation
PBS, 1mg/ml BSA, 0.02% sodium azide.
State of Matter
Liquid
Product Preparation
Purified by immunoaffinity chromatography
Storage and Handling
Store at 2-8°C for up to 3 months or at -20°C for at least one (1) year. Store in appropriate aliquots to avoid multiple freeze-thaw cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Applications and Recommended Usage?
Quality Tested by Leinco


Western Blot: use at 1ug/ml, detection limit 0.8ng TetR

ELISA: detection limit 0.2ng TetR.

End users should determine optimal concentrations for their applications. Not all applications have been investigated.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
Rabbit Polyclonal Antibody specific to Tet Repressor
Background
The Tet repressor protein (TetR) regulates transcription of a family of tetracycline resistance determinants in Gram-negative bacteria. The Tet system is a widely used regulatory system for conditional gene expression in eukaryotic cells and can be used for that purpose in some organisms without further modifications. However, in mammalian cells and in other organisms, eukaryotic transcriptional activator or repressor domains are fused to TetR to turn it into an efficient regulator.

Antigen Details

Research Area
DNA

References & Citations

Indirect Elisa Protocol
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.