Armenian Hamster IgG Isotype Control F(ab’)2 fragment [Clone PIP] — Purified in vivo GOLD™ Functional Grade
Armenian Hamster IgG Isotype Control F(ab’)2 fragment [Clone PIP] — Purified in vivo GOLD™ Functional Grade
Product No.: I-140-FAB2
Clone PIP Formats AvailableView All Product Type F(ab')2 Isotype Control Isotype Armenian Hamster IgG Applications FC , in vivo |
Antibody DetailsProduct DetailsHost Species Armenian Hamster Recommended Dilution Buffer Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C Working Concentration This isotype control antibody should be used at the same concentration as the primary antibody. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This Armenian Hamster IgG isotype control monoclonal antibody has been tested against selected species' cells and tissues to assure minimal cross-reactivity. Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Common In Vivo Applications of Clone PIP in MiceDefinition and Nature of Clone PIP The term "clone PIP" in this context refers to the Armenian Hamster IgG isotype control antibody clone PIP, produced by Leinco Technologies. It is important to note that PIP is not a specific target antigen, but rather an isotype control: a non-reactive antibody matched to the class (e.g., IgG) and type (e.g., Armenian Hamster) of the primary antibody used in experiments, intended to control for non-specific binding and background signal. However, there seems to be some confusion or ambiguity in the literature referenced here: several sources mention that "clone PIP (prolactin-inducible protein) is primarily used to investigate its roles in immunity, cancer progression, and host defense by employing both PIP knockout mice and syngeneic murine tumor models with PIP-expressing cell lines". This suggests a conflation between the Armenian Hamster IgG clone PIP (an isotype control) and the prolactin-inducible protein (PIP), which is an unrelated protein that participates in human breast cancer biology and has been studied in mouse models via knockout and overexpression systems. Clarifying the ContextIsotype Control Antibody (Clone PIP)
Prolactin-Inducible Protein (PIP) Gene Product
Summary Table: Clone PIP vs. PIP Protein
Key Takeaways
If you are seeking applications specifically for the Armenian Hamster IgG clone PIP, its in vivo use is strictly as a non-reactive, isotype-matched control in antibody experiments. If you are interested in prolactin-inducible protein (PIP) and its biological roles in mice, the focus is on gene knockout and overexpression models to study cancer, immunity, and host defense. Clarification from the original source is recommended if the distinction is unclear. Commonly used antibodies or proteins studied with PIP (which can refer to either phosphatidylinositol phosphates or prolactin-induced protein, depending on context) are typically chosen based on the specific PIP species and research focus. Here are examples for both major contexts: 1. Phosphatidylinositol Phosphates (PIPs) in Cell Signaling/TraffickingAntibodies and proteins frequently used in literature alongside PIPs (like PI4P, PI(4,5)P2, and PI(3,4,5)P3) include:
2. Prolactin-Induced Protein (PIP) in Cancer/Immunology
Summary Table: Commonly Used Antibodies/Proteins with PIP
If your context is specifically phosphatidylinositol phosphates or prolactin-induced protein, the precise set of companion markers or antibodies may differ as shown above. Key findings from scientific literature referencing “clone PIP” or related “PIP” clones show the molecule’s roles in immunoregulation, cancer biology, cell signaling, virulence, and biotechnology. Key findings include:
Summary Table of Core Roles Identified from “clone PIP” Citations
These findings illustrate clone PIP’s diverse scientific relevance, spanning basic immunology, cancer research, microbial pathogenesis, and biotechnological tool development. Based on the available search results, there is limited specific information about dosing regimens for clone PIP across different mouse models. The search results primarily discuss dosing regimens for piperacillin/tazobactam (PIP/TAZ) antibiotics and other drugs in mouse models, rather than clone PIP as a biological reagent. Piperacillin/Tazobactam Dosing in Mouse ModelsFor antimicrobial studies using piperacillin/tazobactam in mice, researchers have developed humanized dosing regimens to mimic clinical therapeutic exposure. One established protocol involves administering multiple subcutaneous doses over a 6-hour period: mice receive TZP 500/62.5 mg/kg at baseline, followed by 200/25 mg/kg at 25 minutes, and 100/12.5 mg/kg at 2.5 hours. Another study utilized PIP dosing at either 120 or 240 mg/kg across three different regimens. General Principles for Mouse DosingThe search results indicate that dosing regimens often require higher doses on a mg/kg basis in mice compared to humans to achieve similar pharmacokinetic and pharmacodynamic parameters. This is because mice have different metabolism and physiology compared to humans, affecting drug clearance rates and exposure times. Limited Information on Clone PIPWhile one search result mentions clone H22 (a murine hepatoma cell line) having varying dosing regimens across different mouse models, specific details about clone PIP dosing strategies are not provided in the available sources. Without additional specific information, it is not possible to provide comprehensive details about how clone PIP dosing regimens vary across different mouse model applications. References & Citations1.) Schreiber, RD. et al. (2017) Cancer Immunol Res. 5(2):106-117. PubMed 2.) Oldstone, MBA. et al. (2017) Proc Natl Acad Sci U S A. 114(14): 3708–3713. PubMed 3.) Schreiber, RD. et al. (2015) PLoS One.10(5):e0128636. PubMed 4.) Diamond, MS. et al. (2017) J Virol. 91(22): e01419-17. PubMed 5.) Gubin, M. et al. (2018) Cell. 175(4):1014–1030.e19 Journal Link 6.) Czepielewski, R. et al. (2021) Immunity 54(12):2795-2811.e9 Journal Link 7.) Winkler, E. et al. (2020) Cell 182(4):901-918.e18 Journal Link Technical ProtocolsCertificate of Analysis |
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