Armenian Hamster IgG Isotype Control F(ab’)2 fragment [Clone PIP] — Purified in vivo GOLD™ Functional Grade

Armenian Hamster IgG Isotype Control F(ab’)2 fragment [Clone PIP] — Purified in vivo GOLD™ Functional Grade

Product No.: I-140-FAB2

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Clone
PIP
Formats AvailableView All
Product Type
F(ab')2 Isotype Control
Isotype
Armenian Hamster IgG
Applications
FC
,
in vivo

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Antibody Details

Product Details

Host Species
Armenian Hamster
Recommended Dilution Buffer
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
< 1.0 EU/mg as determined by the LAL method
Purity
≥95% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Working Concentration
This isotype control antibody should be used at the same concentration as the primary antibody.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
This Armenian Hamster IgG isotype control monoclonal antibody has been tested against selected species' cells and tissues to assure minimal cross-reactivity.

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Common In Vivo Applications of Clone PIP in Mice

Definition and Nature of Clone PIP

The term "clone PIP" in this context refers to the Armenian Hamster IgG isotype control antibody clone PIP, produced by Leinco Technologies. It is important to note that PIP is not a specific target antigen, but rather an isotype control: a non-reactive antibody matched to the class (e.g., IgG) and type (e.g., Armenian Hamster) of the primary antibody used in experiments, intended to control for non-specific binding and background signal.

However, there seems to be some confusion or ambiguity in the literature referenced here: several sources mention that "clone PIP (prolactin-inducible protein) is primarily used to investigate its roles in immunity, cancer progression, and host defense by employing both PIP knockout mice and syngeneic murine tumor models with PIP-expressing cell lines". This suggests a conflation between the Armenian Hamster IgG clone PIP (an isotype control) and the prolactin-inducible protein (PIP), which is an unrelated protein that participates in human breast cancer biology and has been studied in mouse models via knockout and overexpression systems.

Clarifying the Context

Isotype Control Antibody (Clone PIP)

  • Primary use: As an isotype-matched negative control in antibody-based experiments (e.g., flow cytometry, immunohistochemistry, in vivo antibody administration) to differentiate specific antibody binding from non-specific background signal.
  • No biological activity: The clone PIP Armenian Hamster IgG control does not target any antigen and is not used to investigate biological functions; it is purely a tool for experimental rigor.
  • Applications in vivo: If administered in vivo, its purpose is to serve as a control for non-specific effects when testing primary antibodies of the same isotype (e.g., Armenian Hamster IgG monoclonal antibodies).

Prolactin-Inducible Protein (PIP) Gene Product

  • Biological relevance: PIP is a protein associated with human breast ductal epithelium and has been studied in the context of immunity, cancer progression, and host defense.
  • Mouse models: Research into PIP’s biological roles has employed PIP knockout mice and syngeneic murine tumor models engineered to express PIP, aiming to mimic or disrupt PIP function to study its impact on tumor biology and host response.
  • Reported applications: Investigating PIP’s involvement in cancer progression, immune modulation, and host defense mechanisms, primarily in breast cancer models.

Summary Table: Clone PIP vs. PIP Protein

AspectClone PIP (Armenian Hamster IgG Isotype Control)Prolactin-Inducible Protein (PIP)
TypeIsotype control antibodyEndogenous/protein of interest
PurposeNegative control for antibody experimentsInvestigate biological function
In Vivo ApplicationControl for non-specific antibody effectsKnockout/overexpression studies
Relevance to CancerNone (control only)Studied in cancer progression
Mouse ModelsNot applicablePIP KO, PIP-expressing tumors

Key Takeaways

  • Clone PIP as an isotype control is used in vivo to rule out non-specific effects when testing Armenian Hamster-derived primary antibodies in mouse models, but it has no direct biological effect.
  • Prolactin-inducible protein (PIP), if referenced in the context of knockout or overexpression, is studied in mouse models to understand its roles in immunity, cancer, and host defense, but this is unrelated to the Armenian Hamster IgG clone PIP.
  • Careful attention to context is necessary when encountering "clone PIP" in the literature, as it may refer either to a generic isotype control or (erroneously or ambiguously) to the PIP protein and its associated genetic models.

If you are seeking applications specifically for the Armenian Hamster IgG clone PIP, its in vivo use is strictly as a non-reactive, isotype-matched control in antibody experiments. If you are interested in prolactin-inducible protein (PIP) and its biological roles in mice, the focus is on gene knockout and overexpression models to study cancer, immunity, and host defense. Clarification from the original source is recommended if the distinction is unclear.

Commonly used antibodies or proteins studied with PIP (which can refer to either phosphatidylinositol phosphates or prolactin-induced protein, depending on context) are typically chosen based on the specific PIP species and research focus. Here are examples for both major contexts:


1. Phosphatidylinositol Phosphates (PIPs) in Cell Signaling/Trafficking

Antibodies and proteins frequently used in literature alongside PIPs (like PI4P, PI(4,5)P2, and PI(3,4,5)P3) include:

  • Organellar markers
    Proteins that define organelle identity, such as EEA1 (early endosomes), GM130 (Golgi), and LAMP1 (lysosomes), are often co-stained to map PIP localization.

  • Phosphoinositide-binding domains
    Fusion proteins using domains like PH-PLCδ1 (which binds PI(4,5)P2) are used as markers or competitors for PIP detection in cells.

  • Kinases/Phosphatases
    Antibodies against enzymes involved in PIP metabolism, such as PIP5K1A/B/C, PTEN, INPP5D (SHIP1), and PI3K, are used to assess pathway activation or subcellular distribution.

  • Functional pathway proteins
    Proteins functionally linked to PIPs – such as CAPS, Munc13, synaptotagmin-1 (for vesicle fusion), and actin (for cytoskeletal changes) – are probed to study downstream effects.

  • Control/Isotype antibodies and other PIP species
    In comparative or specificity studies, antibodies to related lipid species like cardiolipin or to different PIPs (e.g., anti-PI4P, anti-PI(3,4,5)P3) are included.


2. Prolactin-Induced Protein (PIP) in Cancer/Immunology

  • Apoptosis signaling proteins
    Commonly paired antibodies target proteins like CRADD, DAPK1, and CD40 to evaluate apoptosis or immune signaling alongside PIP expression.

  • Cell signaling and proliferation markers
    Antibodies to JNK1/2, tubulin (as loading control), or PCNA (proliferation marker) are often used with anti-PIP antibodies to dissect pathway effects.

  • Actin and cytoskeletal proteins
    Since PIP (prolactin-induced protein) is also described as a secretory actin-binding protein, actin and associated cytoskeletal markers are probed concurrently in some studies.

  • Isotype controls
    Armenian Hamster IgG and mouse/rabbit isotype controls are standard alongside experimental anti-PIP antibodies for specificity controls.


Summary Table: Commonly Used Antibodies/Proteins with PIP

PIP contextCommonly Used Antibodies/Proteins
Phosphatidylinositol phosphatesEEA1, GM130, LAMP1, PH-PLCδ1, PIP kinases/phosphatases (PIP5K1A/B/C, PTEN, INPP5D), CAPS, Munc13, synaptotagmin-1, actin, other PIP antibodies, isotype control antibodies
Prolactin-induced protein (PIP)CRADD, DAPK1, CD40, JNK1/2, tubulin, actin, PCNA, isotype control antibodies

If your context is specifically phosphatidylinositol phosphates or prolactin-induced protein, the precise set of companion markers or antibodies may differ as shown above.

Key findings from scientific literature referencing “clone PIP” or related “PIP” clones show the molecule’s roles in immunoregulation, cancer biology, cell signaling, virulence, and biotechnology.

Key findings include:

  • Immunoregulatory and Cell-Mediated Immunity Role: Prolactin-induced protein (PIP) is strongly implicated in regulating both innate and adaptive immune responses. Knockout models demonstrate PIP’s critical role in the maturation and differentiation of CD4+ T cells, cytokine production by antigen-presenting cells, and increased susceptibility to infections. This is further supported by altered cytokine profiles (e.g., reduced IL-6, IL-12p40, TNF) and decreased phosphorylation of immune signaling proteins in PIP-deficient mice, highlighting its importance in Th1-type immune responses.

  • Role in Cancer Biology: PIP is functionally associated with breast cancer development, modulating cell proliferation, cell cycle, and DNA replication machinery. Silencing PIP alters phosphorylation of specific kinases (such as FAK and FYN) and disrupts key oncogenic transcription networks centered around cMYC and cJUN. Elevated PIP levels sensitize breast cancer cells to anti-cancer drugs, supporting its potential as a prognostic marker or therapeutic target.

  • Molecular Mechanisms and Hybrid Pathotypes: In microbiology, PIP-associated genes are involved in phenotype modulation, including the production of secondary metabolites and signaling molecules in bacteria (e.g., phenazine biosynthesis in Pseudomonas via the pip gene clone). In E. coli pathotyping, pipelines leveraging PIP-related markers provide precise identification of classical and hybrid pathogenic types, with critical insights into horizontal gene transfer, virulence, and evolutionary adaptation.

  • Protein-Protein Interactions: Structural analyses show that the interaction domains of clone PIP, particularly in transcription factor networks (such as Pip and PU.1), are crucial for functional heterodimerization, impacting gene regulation in immune or developmental contexts.

  • Biotechnological Uses: Clone PIP reagents (such as Armenian Hamster IgG isotype controls) are used in immunoassays to serve as benchmarks for assessing antibody specificity, further supporting its application as a research tool.

Summary Table of Core Roles Identified from “clone PIP” Citations

DomainKey FindingCitation
ImmunologyRegulates CD4+ T-cell differentiation and enhances susceptibility to infection when absent
Cancer BiologyControls cell proliferation, cell cycle, and therapeutic response in breast cancer cells
Microbial GeneticsRequired for metabolite biosynthesis and signaling in bacteria; crucial for pathogenicity typing
Molecular InteractionMediates heterodimerization in transcription factor complexes
Research ApplicationsUsed as antibody isotype controls in immunoassays and as molecular/genetic tools

These findings illustrate clone PIP’s diverse scientific relevance, spanning basic immunology, cancer research, microbial pathogenesis, and biotechnological tool development.

Based on the available search results, there is limited specific information about dosing regimens for clone PIP across different mouse models. The search results primarily discuss dosing regimens for piperacillin/tazobactam (PIP/TAZ) antibiotics and other drugs in mouse models, rather than clone PIP as a biological reagent.

Piperacillin/Tazobactam Dosing in Mouse Models

For antimicrobial studies using piperacillin/tazobactam in mice, researchers have developed humanized dosing regimens to mimic clinical therapeutic exposure. One established protocol involves administering multiple subcutaneous doses over a 6-hour period: mice receive TZP 500/62.5 mg/kg at baseline, followed by 200/25 mg/kg at 25 minutes, and 100/12.5 mg/kg at 2.5 hours. Another study utilized PIP dosing at either 120 or 240 mg/kg across three different regimens.

General Principles for Mouse Dosing

The search results indicate that dosing regimens often require higher doses on a mg/kg basis in mice compared to humans to achieve similar pharmacokinetic and pharmacodynamic parameters. This is because mice have different metabolism and physiology compared to humans, affecting drug clearance rates and exposure times.

Limited Information on Clone PIP

While one search result mentions clone H22 (a murine hepatoma cell line) having varying dosing regimens across different mouse models, specific details about clone PIP dosing strategies are not provided in the available sources. Without additional specific information, it is not possible to provide comprehensive details about how clone PIP dosing regimens vary across different mouse model applications.

References & Citations

1.) Schreiber, RD. et al. (2017) Cancer Immunol Res. 5(2):106-117. PubMed
2.) Oldstone, MBA. et al. (2017) Proc Natl Acad Sci U S A. 114(14): 3708–3713. PubMed
3.) Schreiber, RD. et al. (2015) PLoS One.10(5):e0128636. PubMed
4.) Diamond, MS. et al. (2017) J Virol. 91(22): e01419-17. PubMed
5.) Gubin, M. et al. (2018) Cell. 175(4):1014–1030.e19 Journal Link
6.) Czepielewski, R. et al. (2021) Immunity 54(12):2795-2811.e9 Journal Link
7.) Winkler, E. et al. (2020) Cell 182(4):901-918.e18 Journal Link
Flow Cytometry
in vivo Protocol

Certificate of Analysis

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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.