Recombinant Human Ephrin-A5

Pricing & Details

Product. No.E200
Alternate Names
AL-1, RAGS, LERK-7, ERL-5, EFNA5, AF1, EPLG7, GLC1M
Expression Host
NS0 cells
Reactivity Species
Human
Prod No.
Size
Price
Avail.
Qty
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E200-200 µg
200 µg
$279.00
In stock
Max:
Min: 1
Step: 1

Background

Ephrin-A5, also known as EFNA5, is a member of the ephrin gene family that prevents axon bundling in cocultures of cortical neurons with astrocytes, a model of late stage nervous system development and differentiation. The EPH and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, particularly in the nervous system. EPH receptors typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats.

Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are similarly divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin-A5 is required for the proper guidance and mapping of retinal axons in the mammalian midbrain.1

Protein Details

Purity
>90% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Fusion Protein Tag
Fc Fusion Protein
Expression Host
NS0 cells
Protein Accession No.
Amino Acid Sequence
qdpgskavad ryavywnssn prfqrgdyhi dvcindyldv fcphyedsvp edkteryvly mvnfdgysac dhtskgfkrw ecnrphspng plkfsekfql ftpfslgfef rpgreyfyis saipdngrrs clklkvfvrp tnscmktigv hdrvfdvndk venslepadd tvhesaepsr geniegrmdp kscdkthtcp pcpapellgg psvflfppkp kdtlmisrtp evtcvvvdvs hedpevkfnw yvdgvevhna ktkpreeqyn styrvvsvlt vlhqdwlngk eykckvsnka lpapiektis kakgqprepq vytlppsrde ltknqvsltc lvkgfypsdi avewesngqp ennykttppv ldsdgsffly skltvdksrw qqgnvfscsv mhealhnhyt qkslslspgk
N-terminal Sequence Analysis
No results obtained: Gln21 predicted
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human Ephrin-A5 is Mr 48.6 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 50-55 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Barbacid, M. et al. (1998) Neuron 20: 235
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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