Purified Human IgM, Fc5μ Fragment (>98%)
This secondary antibody is lyophilized from 0.01M Tris buffered saline (TBS) pH 8.0 containing 0.25M NaCl, 15 mg/ml IgG free BSA and 0.05% sodium azide as a preservative.
This F(ab')2 secondary antibody was generated by optimal pepsin degestion of the antisera followed by antigen affinity chromatography to elimiate the presence of Fc fragment and whole molecules.
Storage and Handling
This lyophilized secondary antibody conjugate should be stored desiccated at 2-8°C. The reconstituted Alkaline Phosphatase (Alk Phos) conjugate containing glycerol should be stored at -20°C in a manual defrost freezer.
One year from date of reconstitution
Country of Origin
Next Day Ambient
It is recommended that working dilutions be prepared fresh daily. WB: 1:5,000 - 1:50,000ELISA: 1:5,000 - 1:50,000Secondary antibody concentrations reconstituted with glycerol will be diluted by 50%. Therefore, the working dilution may be one half of what is recommended.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.
Goat F(ab')2 Anti-Human IgM, Fc5μ Fragment recognizes Human IgM, Fc5μ Fragment. This secondary antibody was purified using antigen affinity chromatography and has been adsorbed against serum proteins and immunoglobulins to minimize species cross-reactivity.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.