HEPES buffered saline with EDTA (10X)

Pricing & Details

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Prod No.
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H1679-4 x 1L
4 x 1L
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HEPES is a zwitterionic (“hybrid”) organic chemical buffering agent; one of the twenty Good’s Buffers excellence in early discovery research HEPES Buffered Saline with EDTA (10X) Cell Culture Buffer & Flow Cytometry Sorting Reagents which derives from a set of buffers described by Dr. Norman Good and his colleagues in 1966 (Good et.al., Biochemistry 1966). HEPES is widely used in cell culture because it is better at maintaining a physiological pH compared to bicarbonate buffers and provides extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO2 incubator. "Good’s Buffers" and HEPES are associated with the following characteristics: - pKa values between 6.0 and 8.0 - high solubility - membrane impermeability - limited effect on biochemical reactions - very low visible and ultraviolet light absorbance (great for Flow Cytometry applications) - chemically and enzymatically stable

Manufacturing Standards

All Leinco Technologies PBS and sheath fluid solutions are specially manufactured in our class 10,000 clean room environment located in St. Louis, Missouri. Each lot is packaged under our ISO 9001:2015 and cGMP quality standards. For custom options and improved supply chain management/continuity please contact Leinco for custom bulk options and auto-ship programs.

Product Specifications

pH Range
10X Liquid Concentrate
Sterile filtered and aseptically packaged, contains EDTA
Components Molecular Weight Concentration (mg/L) mM
HEPES (C8H18N2O4S) 136.09 300 1.0581
Sodium Chloride (NaCl) 58.44 8,800 154
EDTA 141.96 452.2 5.6
Note: Does not contain calcium, magnesium, sodium pyruvate or phenol red.

Storage and Stability

Store Leinco sheath fluids at room temperature. DO NOT FREEZE. Stable when stored as directed for 12 months from date received.

Country of Origin

Products are for research use only. Not for use in diagnostic or therapeutic procedures.