Lysosome Associated Membrane Protein-1 Blocking Peptide

15 amino acids near the center of human LAMP-1.

This peptide is formulated in PBS pH 7.2 (0.01 M Sodium Phosphate, 0.13 M NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide.

Store this peptide in working aliquots at -20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Next Day Ambient

15 amino acids near the center of human LAMP-1.

Lysosome Associated Membrane Protein 1 peptide is applicable as a control peptide for blocking antibody binding in Western blotting.

1. Chen JW, Murphy TL, Willingham MC, et al. Identification of two lysosomal membrane glycoproteins. J. Cell Biol. 1985; 101:85-95. 2. Kisen GO, Tessitore L, Costelli P, et al. Reduced autophagic activity in primary rat hepatocellular carcinoma and ascites hepatoma cells. Carcinogenesis 1993; 14:2501-5. 3. Kamada Y, Funakoshi T, Shintani T, et al. Tor-mediated induction of autophagy via Apg1 protein kinase complex. J. Cell. Biol. 2000; 150:1507-13. 4. Granger BL, Green SA, Gabel CA, et al. Characterization and cloning of the lgp110, a lysosomal glycoprotein from mouse and rat cells. J. Biol. Chem. 1990; 265:12036-43. 5. Tanaka Y, Guhde G, Suter A, et al. Accumulation of autophagic vacuoles and cardiopathy in LAMP-2-deficient mice. Nature 2000; 902-6. 6. Nishino I, Fu J, Tanji K, et al. Primary LAMP-2 deficiency causes X-linked vacuolar cardiomyopathy and myopathy (Danon disease). Nature 2000; 906-10. 7. Gough NR, Hatem CL, and Fambrough DM. The family of LAMP-2 proteins arises by alternative splicing from a single gene: characterization of the avian LAMP-2 gene and identification of mammalian homologs of LAMP-2b and LAMP-2c. DNA Cell Biol. 1995; 14:863-7.