Mouse IgG2a Isotype Control [Clone C1.18.4] — Purified in vivo GOLD™ Functional Grade
Antibody DetailsProduct DetailsHost Species Mouse Recommended Isotype Controls Recommended Dilution Buffer Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C Working Concentration This isotype control antibody should be used at the same concentration as the primary antibody. RRIDAB_2737531 Applications and Recommended Usage? Quality Tested by Leinco FC This isotype control antibody should be used at the same concentration as the primary antibody. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This Mouse IgG2a (Anti-KLH) isotype control antibody has been tested against selected species' cells and tissues to assure minimal cross reactivity. The isotype of this antibody is Mouse IgG2a, κ. This antibody was chosen as an isotype control after screening on a variety of resting, activated, live, and fixed mouse, rat and human tissue Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone C1.18.4 is a mouse IgG2a isotype control antibody that serves as a negative control in various in vivo mouse studies rather than targeting specific antigens. Its primary function is to account for non-specific binding and background effects in experimental settings where antigen-specific mouse IgG2a antibodies are being tested. Primary Role in In Vivo ResearchThe C1.18.4 clone functions as an isotype-matched negative control to distinguish specific biological effects from non-specific antibody interactions. This antibody has been specifically tested against mouse cells and tissues to ensure minimal cross-reactivity, making it particularly suitable for in vivo mouse experiments. Common Experimental ApplicationsIn mouse studies, C1.18.4 is used to control for potential confounding factors including:
Researchers typically administer C1.18.4 alongside the test antibody to separate target-specific effects from these non-specific responses. Technical Specifications for In Vivo UseThe clone is manufactured to meet stringent requirements for in vivo applications, including low endotoxin levels (less than 1.0 EU/mg or even below 0.5 EU/mg for ultra-low endotoxin versions). The antibody achieves high purity (>95%) through protein A or G affinity chromatography and undergoes 0.2 µm filtration for sterility. These specifications are critical for minimizing inflammatory responses and other artifacts in living animals. Fc-Modified VariantsA specialized version of C1.18.4 with LALA-PG mutations is available for experiments requiring Fc silencing. This Fc-muted variant eliminates effector functions while maintaining the isotype control properties, useful when researchers need to isolate antigen-binding effects from Fc-mediated immune responses. Commonly Used Antibodies and Proteins Alongside C1.18.4C1.18.4 is a well-characterized mouse monoclonal antibody of the IgG2a, kappa isotype, used almost exclusively as a negative (isotype-matched) control in immunological and cell biology experiments. Its primary function is to provide a benchmark for non-specific binding and background signal when evaluating antigen-specific antibodies of the same isotype. Typical Experimental Pairings
Example Applications in the Literature
Summary Table: Typical Antibodies Used with C1.18.4
Key Points
If you have a specific experimental context or citation, more tailored examples of antibody pairings may be available. For the most common uses, however, the above covers the typical antibodies and proteins used alongside C1.18.4 in the literature. Clone C1.18.4 is a mouse IgG2a isotype control antibody used extensively in immunological and biomedical research to control for non-specific binding—rather than to study any biological target or effect itself. Key findings from its citations in scientific literature focus on its role as an experimental control, not as a probe for a biological process or marker. Key supported roles and findings:
Nature of Citations:
Summary Table: Key Points about Clone C1.18.4
If you are seeking findings related to biological activity, mechanism, or phenotype linked specifically to C1.18.4, no such results exist in scientific literature; citations exclusively validate its use as a negative control. Dosing Regimens of Clone C1.18.4 in Mouse ModelsClone C1.18.4 is a mouse IgG2a isotype control antibody, widely used as a negative control in immunological experiments to assess non-specific binding and establish baseline immune responses. Its primary utility lies in experimental control rather than therapeutic targeting, which influences how it is used and dosed in mouse studies. General Dosing Approach
Specific Examples and Variability
Key Considerations
Summary Table
ConclusionDosing regimens for clone C1.18.4 in mouse models are not fixed but are instead tailored to match the administration protocol of the experimental antibody under investigation. The primary goal is to provide a valid negative control, ensuring that any observed effects can be confidently attributed to the specific action of the test antibody rather than non-specific immune modulation. Researchers should carefully document the dosing strategy used in their specific model to ensure reproducibility and accurate data interpretation. References & Citations1.) Ikeda Y. et al. (2003) Blood.101(2):621-3. Article Link 2.) Wright, TM et al. (2000) J Immunol.164(12):6138-46.PubMed 3.) Wright, TM et al. (2001) Arthritis Rheum. 44(7):1654-9. PubMed 4.) Nishikawa, Takeji et al. (2000) Journal of Investigative Dermatology 114(1): 88-94. PubMed 5.) Gubin et al. (2018) Cell. 175:1014–1030 Journal Link Technical ProtocolsCertificate of Analysis |
Formats Available
Prod No. | Description |
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I-923 | |
I-105 | |
I-129 | |
I-915 | |
I-916 | |
I-917 | |
I-919 | |
I-921 | |
I-107 | |
I-118 | |
I-106 | |
I-134 | |
P381 |
