Mouse IgG2b Isotype Control [Clone MPC-11] — Purified in vivo PLATINUM™ Functional Grade

Mouse IgG2b Isotype Control [Clone MPC-11] — Purified in vivo PLATINUM™ Functional Grade

Product No.: M1415

[product_table name="All Top" skus="M1415"]

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Clone
MPC-11
Target
unknown binding specificity
Formats AvailableView All
Product Type
Isotype Control
Isotype
Mouse IgG2b
Applications
FA
,
FC
,
ICC
,
ICFC
,
IF Microscopy
,
IF Staining
,
in vivo
,
IP
,
WB

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Select Product Size
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Antibody Details

Product Details

Host Species
Mouse
Recommended Dilution Buffer
Product Concentration
≥ 5.0 mg/ml
Endotoxin Level
<0.5 EU/mg as determined by the LAL method
Purity
≥98% monomer by analytical SEC
>95% by SDS Page
Formulation
This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.
Product Preparation
Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Pathogen Testing
To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile.
Storage and Handling
Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles.
Country of Origin
USA
Shipping
Next Day 2-8°C
Working Concentration
This isotype control antibody should be used at the same concentration as the primary antibody.
Applications and Recommended Usage?
Quality Tested by Leinco
FC This isotype control antibody should be used at the same concentration as the primary antibody.
Additional Applications Reported In Literature ?
FA
ICC
ICFC
IF Microscopy
IF Staining
WB
For specific conjugates of this clone, review literature for suggested application details.
Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change.

Description

Specificity
This Mouse Mouse isotype control antibody has been tested against selected species' cells and tissues to assure minimal cross reactivity.

Leinco Antibody Advisor

Powered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments.

The clone MPC-11 is used in in vivo mouse studies primarily as a mouse IgG2b isotype control antibody. Its main function is to serve as a negative control to measure nonspecific binding or background signal when testing other mouse IgG2b monoclonal antibodies in live animal studies.

Key points about its use:

  • Non-reactive isotype control: MPC-11 is selected for its lack of known target specificity, making it ideal for distinguishing between true biological effects of a specific test antibody and non-specific effects due to the antibody isotype itself.
  • Experimental application: In in vivo studies, it is administered to mice in parallel with a test antibody to control for effects unrelated to the target antigen, such as Fc receptor-mediated effects or immune activation due solely to antibody presence.
  • Quality and purity: The antibody is available with low endotoxin levels (<2 EU/mg, or even lower in some preparations), which is important for minimizing unintended immune activation in live mice.
  • Model system: It is particularly used in experiments involving mouse IgG2b isotype antibodies—such as studies of immunotherapy, tumor growth, or immune cell depletion—to ensure observed effects are due to target-specific action.
  • Administration: MPC-11 is typically administered via the same route, dose, and regimen as the test antibody in animal models.

MPC-11 is not used to target or neutralize any specific protein in mice; it is strictly a control tool for in vivo experimental rigor.

The MPC-11 antibody, which is a mouse IgG2b isotype control, is commonly used in various immunological assays as a negative control to assess non-specific binding. Here are some other commonly used antibodies or proteins that might be used in conjunction with MPC-11 in the literature:

  1. Polyclonal Antibodies against Murine Plasmacytoma Cell Globulins: These are used in studies involving myeloma cells, such as MPC-11 cells themselves, to target multiple antigens on these cells.

  2. Goat Anti-Rabbit Secondary Antibodies: These are often used in ELISA and Western blotting assays where rabbit-derived primary antibodies are employed.

  3. Caspase-3, Caspase-8, and Caspase-9 Antibodies: These are used to study apoptosis pathways in cells like MPC-11, especially in Western blotting.

  4. Other Isotype Controls: Depending on the specific antibody being used in an experiment, other isotype controls like IgG1, IgG2a, or IgM might be used alongside MPC-11 to ensure that any observed effects are specific to the antigen-targeting antibody rather than non-specific binding.

  5. Antibodies for Flow Cytometry: When performing flow cytometry, antibodies specific to cell surface antigens are often used in conjunction with isotype controls like MPC-11 to validate staining specificity.

These antibodies and proteins help ensure the specificity and reliability of the results obtained in immunological experiments.

Key findings from clone MPC-11 citations in scientific literature center around its use as a murine plasmacytoma cell line to study immunoglobulin heavy chain genetics, plasma cell biology, tumor progression, and therapeutic response:

  • Immunoglobulin Heavy Chain Structure: Cloning and sequencing efforts for MPC-11 (notably using cDNA of its heavy chain variable region) revealed that the variable region contains 121 amino acids, falls into subgroup II of mouse heavy chains, and features a J (joining) segment of 16 residues overlapping the third hypervariable region. Importantly, there is no discontinuity between the end of the variable region and the start of the constant region, clarifying the genetic arrangement in this model.

  • Model for Plasma Cell Malignancy: MPC-11 is a widely used syngeneic mouse cell line model for studying plasma cell biology, tumor progression, and testing cancer therapeutics, including chemotherapy, immunotherapy, and targeted treatments. Researchers utilize MPC-11 to explore genetic alterations, signaling pathways, immune responses, and drug sensitivity in plasmacytoma research.

  • Class Switching in Immunoglobulin Genes: Studies using MPC-11 have demonstrated DNA rearrangements and immunoglobulin heavy chain class switching in vitro. Specifically, the IgG2b-producing MPC-11 cell line can give rise to IgG2a-producing cells, helping elucidate mechanisms of class switch recombination in plasma cells.

  • Tool for Antibody Production: Clone MPC-11 is also used to produce mouse IgG2b, kappa isotype control antibodies, a critical reagent for flow cytometry and immunoassays, validating its utility beyond basic research.

These findings highlight the scientific significance of clone MPC-11 as a model system for genetic, molecular, and translational research on plasma cell tumors and antibody biology.

Dosing regimens for clone MPC-11 (mouse IgG2b monoclonal antibody, commonly used as an isotype control) are typically not standardized across different mouse models, as the antibody is employed as a negative control rather than for direct therapeutic or functional effect. However, dosing practices are informed by the need to match both the dose and administration schedule of the experimental antibody being controlled for, as well as ensuring minimal biological activity from the isotype itself.

Key considerations for dosing MPC-11:

  • Typical Dose Range: 100–250 ?g per mouse per injection is commonly reported for isotype control antibodies of this class in in vivo immunological studies. When used as a control, MPC-11's dose should equal or slightly exceed the dose of the experimental IgG2b antibody being compared, to ensure any observed effects are not due to antibody quantity.
  • Route of Administration: Most frequently delivered by intraperitoneal (IP) injection, consistent with the administration of therapeutic or experimental antibodies in mouse protocols.
  • Dosing Frequency: Often administered on the same schedule as the experimental antibody—for example, every 3–4 days, 2–3 times per week, or at specified intervals corresponding to the immune activation window of the model in use.
  • Mouse Model Variability: The specific mouse model (e.g., tumor-bearing, autoimmune, wild-type) typically does not strongly affect the MPC-11 dose, since it is a non-reactive isotype control designed for negligible biological activity. However, researchers sometimes tailor dosing slightly based on model sensitivity, mouse size (e.g., juvenile versus adult), or to match the therapeutic antibody protocol exactly.
  • Regimen Matching: Investigators should match doses, routes, and schedules of MPC-11 to the experimental antibody in use, adjusting only if the experimental protocol calls for a specific adjustment due to model sensitivity or antibody pharmacokinetics.
ParameterTypical MPC-11 Regimen
Dose100–250 ?g per mouse per injection
RouteIntraperitoneal injection (IP); occasionally intravenous
ScheduleMatched to test antibody (e.g., every 3 days, or 2–3x/week)
Mouse ModelNot protocol-specific; standard dosing unless otherwise noted
PurposeNegative/isotype-matched control (minimal/no biological effect)

Supporting details from supplier and procedural references:

  • Bio X Cell and IchorBio recommend using MPC-11 at doses and schedules identical to experimental IgG2b antibodies.
  • When used for flow cytometry, different concentrations apply, typically ?L/test for cell suspensions, but this differs from in vivo dosing regimens.

Summary:
Dosing regimens for MPC-11 usually do not vary across mouse models; they are instead chosen to precisely match the experimental conditions of the antibody under investigation for proper control. Always consult the experimental protocol and antibody supplier recommendations for final dose selection.

References & Citations

FA
Flow Cytometry
ICC
ICFC
IF Microscopy
IF Staining
in vivo Protocol
Immunoprecipitation Protocol
General Western Blot Protocol

Certificate of Analysis

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Formats Available

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Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.