Mouse IgG2b Isotype Control [Clone MPC-11] — Purified in vivo PLATINUM™ Functional Grade
Mouse IgG2b Isotype Control [Clone MPC-11] — Purified in vivo PLATINUM™ Functional Grade
Product No.: M1415
Clone MPC-11 Target unknown binding specificity Formats AvailableView All Product Type Isotype Control Isotype Mouse IgG2b Applications FA , FC , ICC , ICFC , IF Microscopy , IF Staining , in vivo , IP , WB |
Antibody DetailsProduct DetailsHost Species Mouse Recommended Dilution Buffer Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C Working Concentration This isotype control antibody should be used at the same concentration as the primary antibody. RRIDAB_2831345 Applications and Recommended Usage? Quality Tested by Leinco FC This isotype control antibody should be used at the same concentration as the primary antibody. Additional Applications Reported In Literature ? FA ICC ICFC IF Microscopy IF Staining WB For specific conjugates of this clone, review literature for suggested application details. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This Mouse Mouse isotype control antibody has been tested against selected species' cells and tissues to assure minimal cross reactivity. Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. The clone MPC-11 is used in in vivo mouse studies primarily as a mouse IgG2b isotype control antibody. Its main function is to serve as a negative control to measure nonspecific binding or background signal when testing other mouse IgG2b monoclonal antibodies in live animal studies. Key points about its use:
MPC-11 is not used to target or neutralize any specific protein in mice; it is strictly a control tool for in vivo experimental rigor. The correct storage temperature for sterile packaged clone MPC-11 (commonly a mouse IgG2b ? monoclonal antibody or isotype control) is 2–8°C. It should be protected from prolonged exposure to light and must not be frozen. This storage condition is consistent across multiple vendors and product forms, including sterile liquid antibodies and ready-to-use formats. Some suppliers specifically note that the product is stable for at least one year when stored at 2–8°C, undiluted, and protected from light. The exception is if you are referring to live MPC-11 cells (not the sterile, packaged, antibody product): live cell lines should be stored below -130°C, preferably in the vapor phase of liquid nitrogen. If your product is sterile packaged antibody (not living cells), store at 2–8°C and do not freeze. If it is cryopreserved cells, store in liquid nitrogen vapor phase. The MPC-11 antibody, which is a mouse IgG2b isotype control, is commonly used in various immunological assays as a negative control to assess non-specific binding. Here are some other commonly used antibodies or proteins that might be used in conjunction with MPC-11 in the literature:
These antibodies and proteins help ensure the specificity and reliability of the results obtained in immunological experiments. Key findings from clone MPC-11 citations in scientific literature center around its use as a murine plasmacytoma cell line to study immunoglobulin heavy chain genetics, plasma cell biology, tumor progression, and therapeutic response:
These findings highlight the scientific significance of clone MPC-11 as a model system for genetic, molecular, and translational research on plasma cell tumors and antibody biology. References & CitationsTechnical ProtocolsCertificate of Analysis |
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