Mouse IgG_2b Isotype Control [Clone MPC-11] — Purified in vivo PLATINUM™ Functional Grade

Clone MPC-11 is a mouse IgG2b isotype control antibody widely utilized in mouse research as a negative control to distinguish non-specific background effects from those caused by test antibodies of the same isotype. This antibody, derived from the MPC-11 cell line (a B lymphocyte from a mouse with plasmacytoma), has unknown specificity and is specifically chosen for its minimal cross-reactivity after screening on various resting, activated, live, and fixed cells and tissues.

Primary In Vivo Applications

The main function of clone MPC-11 in in vivo mouse studies is to serve as an isotype-matched control for experiments using mouse IgG2b κ antibodies. When conducting in vivo research, investigators administer MPC-11 at the same concentration as their test antibody to account for non-specific effects that may arise from antibody administration itself, rather than from specific target binding.

Additional Research Applications

Beyond its primary role as an in vivo control, clone MPC-11 is also employed in various laboratory techniques including flow cytometry, where it helps assess non-specific binding to cells. The antibody is suitable for functional assays, immunohistochemistry (both frozen and paraffin sections), Western blotting, immunofluorescence, and immunoprecipitation.

Quality Standards for In Vivo Use

For in vivo applications, clone MPC-11 is manufactured with stringent quality controls, typically achieving endotoxin levels of ≤1-2 EU/mg (with some preparations reaching <0.5 EU/mg) and purity levels of ≥95-98%. These specifications are critical for in vivo studies to minimize confounding effects from contaminants that could trigger immune responses independent of the antibody's intended function.

Commonly used antibodies or proteins in the literature alongside MPC-11 (a mouse IgG2b isotype control antibody) include those that serve as test or experimental antibodies in immunological studies, particularly:

• Anti-PD-1, Anti-PD-L1, and Anti-CTLA-4: These are immune checkpoint inhibitors used in mouse models to study immune modulation. MPC-11 is specifically used as a negative control to match these functionally active IgG2b antibodies, ensuring any observed biological effects are due to the specificity of the test antibody and not the isotype itself.
• Depleting/functional antibodies: When studying immune cell depletion, other mouse IgG2b monoclonals targeting proteins such as CD4, CD8, or specific immune markers are used, with MPC-11 serving as an isotype control for these depletion studies.
• Detection reagents in vitro: In ELISA or Western Blot, common pairs include:
  • Goat anti-rabbit secondary antibodies for detection in assays involving MPC-11 or related cell lines, particularly those antibodies conjugated to alkaline phosphatase or HRP.
  • Caspase-3, Caspase-8, and Caspase-9 antibodies: Used in studies on apoptosis pathways, often with MPC-11 as a control for IgG2b-specific background in Western blotting.
• Fluorescent conjugates for flow cytometry: MPC-11 as an IgG2b control is matched to test antibodies labeled with various fluorochromes, ensuring controls for non-specific binding when using fluorochrome-conjugated experimental antibodies in multicolor flow cytometry panels.

Summary of commonly used antibodies/proteins with MPC-11:

MPC-11 is not used to detect any particular protein; instead, its main function is as a negative control for mouse IgG2b to ensure background staining or non-specific effects can be reliably distinguished from true positive signals produced by the test or experimental IgG2b antibody.

If you are working within a specific experimental context (e.g., immuno-oncology, neuroimmunology, flow cytometry), the commonly paired antibodies will align with the major targets of that research field, but the principles above remain the same.

Clone MPC-11 is a mouse myeloma cell line that has been extensively used in scientific research, particularly in immunology and cancer studies. Here are some key findings from the scientific literature:

1. Immunoglobulin Class Switching: MPC-11 is known for its role in studying immunoglobulin heavy chain class switching. It naturally produces IgG2b but can switch to produce IgG2a, allowing researchers to study class switching mechanisms in vitro.

2. Nonexpression of Heat Shock Genes: The MPC-11 cell line has been found to have a silent major heat shock gene, which does not express even under stress conditions. This is attributed to transcriptional blockage rather than gene deletion or rearrangement.

3. Isotype Control in Research: MPC-11 is widely used as an isotype control for mouse IgG2b antibodies in both in vivo and in vitro applications. It serves as a non-reactive comparator to ensure that any observed effects are due to the specificity of the experimental antibody rather than the IgG2b isotype itself.

4. Genetic Control of Hybrid Resistance: Studies involving MPC-11 have shown that hybrid resistance to this plasmacytoma is controlled by a single gene, contrasting with other tumors that may involve multiple genes.

5. Cancer and Immunology Research: MPC-11 has been instrumental in advancing our understanding of cancer biology and immunology. It is used to model various immunological processes and to study the dynamics of tumor growth and resistance.

Variation in Dosing Regimens of Clone MPC-11 Across Mouse Models

Clone MPC-11 refers to a mouse IgG2b isotype control antibody commonly used to assess nonspecific background binding in immunological assays, particularly as a negative control in preclinical mouse studies. Its dosing in vivo typically matches the dose and schedule of the primary, experimental IgG2b antibody being tested, rather than being adjusted for different mouse models.

General Dosing Principles

• Mirroring Test Antibody: Dosing regimens for MPC-11 are usually selected to precisely mirror the experimental conditions of the primary antibody under investigation. For example, if a study administers a test IgG2b antibody at 200 µg every three days intraperitoneally, MPC-11 would be given at the same dose and frequency.
• No Model-Specific Adjustment: The available guidance and literature do not indicate that MPC-11 dosing varies by mouse model (e.g., BALB/c, C57BL/6, NOD/SCID, etc.). Instead, the focus is on matching the experimental antibody, ensuring that any observed effects are due to the specific activity of the test antibody, not the isotype control.
• Flexibility in Application: While the main principle is to match the experimental antibody, the actual dose and schedule can vary widely depending on the research context—ranging from single high-dose injections to repeated lower doses, depending on the protocol for the primary antibody.

Literature and Technical Guidance

• Product Documentation: Both Leinco and Bio X Cell state that MPC-11 should be used at the same concentration as the primary antibody. This is a standard practice to ensure that any nonspecific effects (e.g., Fc receptor binding, complement activation) are accounted for in the control group.
• No Published Model-Specific Protocols: A review of available product datasheets and published studies did not reveal model-specific dosing recommendations; dosing is protocol-driven, not model-driven.
• Practical Considerations: For flow cytometry and in vitro applications, the concentration may be titrated based on the assay, but for in vivo studies, the dose is protocol-matched to the experimental antibody.

Summary Table: MPC-11 Dosing in Mouse Models

Conclusion

Dosing regimens for clone MPC-11 in mouse models do not generally vary across different strains or tumor models. Instead, MPC-11 is dosed identically to the experimental IgG2b antibody in each study, ensuring that any observed differences are attributable to the specific activity of the test antibody, not the isotype control. Researchers should consult their specific experimental protocol and primary antibody dosing to determine the appropriate MPC-11 regimen.