Mouse IgG2b Isotype Control [Clone MPC-11] — Purified in vivo PLATINUM™ Functional Grade
Mouse IgG2b Isotype Control [Clone MPC-11] — Purified in vivo PLATINUM™ Functional Grade
Product No.: M1415
Clone MPC-11 Target unknown binding specificity Formats AvailableView All Product Type Isotype Control Isotype Mouse IgG2b Applications FA , FC , ICC , ICFC , IF Microscopy , IF Staining , in vivo , IP , WB |
Antibody DetailsProduct DetailsHost Species Mouse Recommended Dilution Buffer Product Concentration ≥ 5.0 mg/ml Endotoxin Level <0.5 EU/mg as determined by the LAL method Purity ≥98% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Pathogen Testing To protect mouse colonies from infection by pathogens and to assure that experimental preclinical data is not affected by such pathogens, all of Leinco’s Purified Functional PLATINUM™ antibodies are tested and guaranteed to be negative for all pathogens in the IDEXX IMPACT I Mouse Profile. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C Working Concentration This isotype control antibody should be used at the same concentration as the primary antibody. RRIDAB_2831345 Applications and Recommended Usage? Quality Tested by Leinco FC This isotype control antibody should be used at the same concentration as the primary antibody. Additional Applications Reported In Literature ? FA ICC ICFC IF Microscopy IF Staining WB For specific conjugates of this clone, review literature for suggested application details. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity This Mouse Mouse isotype control antibody has been tested against selected species' cells and tissues to assure minimal cross reactivity. Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone MPC-11 is a mouse IgG2b isotype control antibody widely utilized in mouse research as a negative control to distinguish non-specific background effects from those caused by test antibodies of the same isotype. This antibody, derived from the MPC-11 cell line (a B lymphocyte from a mouse with plasmacytoma), has unknown specificity and is specifically chosen for its minimal cross-reactivity after screening on various resting, activated, live, and fixed cells and tissues. Primary In Vivo ApplicationsThe main function of clone MPC-11 in in vivo mouse studies is to serve as an isotype-matched control for experiments using mouse IgG2b κ antibodies. When conducting in vivo research, investigators administer MPC-11 at the same concentration as their test antibody to account for non-specific effects that may arise from antibody administration itself, rather than from specific target binding. Additional Research ApplicationsBeyond its primary role as an in vivo control, clone MPC-11 is also employed in various laboratory techniques including flow cytometry, where it helps assess non-specific binding to cells. The antibody is suitable for functional assays, immunohistochemistry (both frozen and paraffin sections), Western blotting, immunofluorescence, and immunoprecipitation. Quality Standards for In Vivo UseFor in vivo applications, clone MPC-11 is manufactured with stringent quality controls, typically achieving endotoxin levels of ≤1-2 EU/mg (with some preparations reaching <0.5 EU/mg) and purity levels of ≥95-98%. These specifications are critical for in vivo studies to minimize confounding effects from contaminants that could trigger immune responses independent of the antibody's intended function. Commonly used antibodies or proteins in the literature alongside MPC-11 (a mouse IgG2b isotype control antibody) include those that serve as test or experimental antibodies in immunological studies, particularly:
Summary of commonly used antibodies/proteins with MPC-11:
MPC-11 is not used to detect any particular protein; instead, its main function is as a negative control for mouse IgG2b to ensure background staining or non-specific effects can be reliably distinguished from true positive signals produced by the test or experimental IgG2b antibody. If you are working within a specific experimental context (e.g., immuno-oncology, neuroimmunology, flow cytometry), the commonly paired antibodies will align with the major targets of that research field, but the principles above remain the same. Clone MPC-11 is a mouse myeloma cell line that has been extensively used in scientific research, particularly in immunology and cancer studies. Here are some key findings from the scientific literature:
Variation in Dosing Regimens of Clone MPC-11 Across Mouse ModelsClone MPC-11 refers to a mouse IgG2b isotype control antibody commonly used to assess nonspecific background binding in immunological assays, particularly as a negative control in preclinical mouse studies. Its dosing in vivo typically matches the dose and schedule of the primary, experimental IgG2b antibody being tested, rather than being adjusted for different mouse models. General Dosing Principles
Literature and Technical Guidance
Summary Table: MPC-11 Dosing in Mouse Models
ConclusionDosing regimens for clone MPC-11 in mouse models do not generally vary across different strains or tumor models. Instead, MPC-11 is dosed identically to the experimental IgG2b antibody in each study, ensuring that any observed differences are attributable to the specific activity of the test antibody, not the isotype control. Researchers should consult their specific experimental protocol and primary antibody dosing to determine the appropriate MPC-11 regimen. References & CitationsTechnical ProtocolsCertificate of Analysis |
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Formats Available
Prod No. | Description |
|---|---|
I-108 | |
I-130 | |
I-119 | |
I-135 | |
M1415 |
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
