Anti-Mouse NKG2A/C/E – Purified in vivo GOLD™ Functional Grade
Anti-Mouse NKG2A/C/E – Purified in vivo GOLD™ Functional Grade
Product No.: N561
Clone 20D5 Target NKG2a Formats AvailableView All Product Type Monoclonal Antibody Alternate Names CD159a, CD159c, CD159e, NKG2C, NKG2E Isotype Rat IgG2a κ Applications B , FC , IHC , in vivo |
Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Recommended Isotype Controls Recommended Dilution Buffer Immunogen CHO transfected cells expressing the C57BL/6 allele of NKG2A and CD94 Product Concentration ≥ 5.0 mg/ml Endotoxin Level < 1.0 EU/mg as determined by the LAL method Purity ≥95% monomer by analytical SEC ⋅ >95% by SDS Page Formulation This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration. Product Preparation Functional grade preclinical antibodies are manufactured in an animal free facility using in vitro cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates. Storage and Handling Functional grade preclinical antibodies may be stored sterile as received at 2-8°C for up to one month. For longer term storage, aseptically aliquot in working volumes without diluting and store at ≤ -70°C. Avoid Repeated Freeze Thaw Cycles. Country of Origin USA Shipping Next Day 2-8°C RRIDAB_2894148 Applications and Recommended Usage? Quality Tested by Leinco Flow Cytometry:For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. An appropriate secondary used would be a goat-anti-mouse IgG2b FITC or brighter fluorochrome when needed. Additional Applications Reported In Literature ? IHC B Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionDescriptionSpecificity Clone 20D5 recognizes an epitope on the mouse NKG2A, NKG2C, and NKG2E isoforms of the CD94/NKG2 heterodimer. DBA/2J mice are CD94-deficient and do not express CD94/NKG2 receptors. Background NKG2A/C/E antibody, clone 20D5, recognizes the NKG2A, NKG2C, and NKG2E isoforms (also known as CD159a, CD159c,m CD159e, respectively) of the NKG2 receptor, belonging to the C-type lectin-like family. NKG2 receptors are expressed at the cell surface as a heterodimer with CD94 and recognize the non-classical class I MHC-1 molecules HLA-E in humans and Qa-1 in mice1-4. NKG2A/C/E are expressed on natural killer (NK) cells, NKT cells, and activated CD8 T cells5-6. Engagement of NKG2A/CD94 transduces an inhibitory signal, blocking NK and CD8 T cell cytotoxicity and promoting self-tolerance7. In contrast, NKG2C/CD94 and NKGE/CD94 are activating receptors8 and bind with lower affinity to HLA-E9. Cancer cells frequently overexpress HLA-E to protect against NK/CD8 T cell killing, and blocking NKG2 receptors in mice promotes anti-tumor immunity and may enhance the cytotoxic potential of other therapeutic antibodies10,11. The NKG2 receptor antibody monalizumab is currently in phase III clinical trials (INTERLINK-1) in combination with cetuximab in patients with recurrent or metastatic squamous cell carcinoma of the head and neck.
Antigen Distribution NKG2A/C/E is expressed on NK cells, NKT cells, and activated CD8 T cells.
Antigen DetailsMolecular Weight 150 kDa Function Inhibitory activity via ITIMs Research Area Cell Biology . Immunology . Signal Transduction . Stem Cell Leinco Antibody AdvisorPowered by AI: AI is experimental and still learning how to provide the best assistance. It may occasionally generate incorrect or incomplete responses. Please do not rely solely on its recommendations when making purchasing decisions or designing experiments. Clone 20D5 is widely used in vivo in mice to study and manipulate the function of natural killer (NK) cells and CD8+ T cells by targeting the NKG2A, NKG2C, and NKG2E receptors, primarily within the context of cancer immunology and immune regulation. Key in vivo applications include:
Summary table of applications:
Important considerations:
In summary, clone 20D5 is primarily used for checkpoint blockade, functional modulation, and characterization of NKG2A/C/E-positive lymphocytes in a variety of in vivo mouse models, especially in cancer and immunotherapy research. Commonly used antibodies or proteins in combination with 20D5 (anti-mouse NKG2A/C/E) in the literature include anti-PD-1, anti-PD-L1, anti-asialo-GM1, and anti-CD8 antibodies. These combinations are primarily employed to study immune modulation and tumor immunity. Key details:
These antibodies are widely chosen because they target pathways or cell types that interact with NKG2A/C/E-expressing cells, allowing precise dissection of immune cell roles in cancer and infection models. Clone 20D5 is a rat monoclonal antibody widely used in immunology research to detect and functionally block mouse NKG2A, NKG2C, and NKG2E receptors, which are members of the C-type lectin-like receptor family expressed primarily on NK and NKT cells. Key findings from scientific literature using 20D5 can be summarized as follows:
In summary, clone 20D5 is foundational for studying NK cell biology, checkpoint inhibition, and immune regulation in murine models, especially in the contexts of cancer and viral immunity. It remains an essential tool for both basic and translational immunological research. Dosing regimens for clone 20D5 (anti-mouse NKG2A/C/E) antibodies in mouse models vary based on the experimental design, mouse strain, and presence of disease (e.g., tumor vs. normal). Standard regimens typically involve intraperitoneal (i.p.) injections with single or repeated doses ranging from 200–300 μg per mouse. Key details on dosing regimens across different mouse models:
Summary Table: Example 20D5 Regimens in Mouse Models
Additional relevant details
In summary:
References & Citations1. Vance R.E., et al. (1998) J. Exp. Med. 188:1841–1848 2. Braud VM, et al. (1998) Nature. 391(6669):795-9 3. Vance RE, et al. (1999) J Exp Med. 190(12):1801-1812 4. Brooks AG, et al. (1997) J Exp Med. 185(4):795-800 5. Gunturi A, Berg RE, Forman J. (2004) Immunol Res. 30(1):29-34 6. Bertone S, et al. (1999) Eur J Immunol. 29(1):23-9 7. Le Dréan E, et al. (1998) Eur J Immunol. 28(1):264-76 8. Lanier LL, et al. (1998) Immunity. 8(6):693-701 9. Kaiser BK, et al. (2005) J Immunol. 174(5):2878-84 10. André P, Denis C, Soulas C, et al. (2018) Cell. 175(7):1731-1743.e13 11. van Montfoort N, Borst L, Korrer MJ, et al. (2018) Cell. 175(7):1744-1755.e15 Technical ProtocolsCertificate of Analysis |
Formats Available
