Recombinant Dengue Virus Serotype 1 NS1 – Purified No Carrier Protein

• Dengue virus is a mosquito-borne flavivirus circulating as four antigenically distinct serotypes (DENV1–4), and non-structural protein 1 (NS1) is a ~50 kDa glycoprotein encoded by all four.
• NS1 exists in three distinct pools during infection: an intracellular, ER-resident form, a membrane-anchored form displayed at the cell surface, and a secreted extracellular form that circulates in patient serum. This characteristic makes it a widely used acute-phase diagnostic marker.
• NS1 is essential for RNA replication, localizing to virus-induced vesicle packets alongside double-stranded RNA and other replication complex components.
• Secreted and surface-associated NS1 is highly immunogenic and engages host innate immune pathways. Reported interactions include complement regulators, TLR4/MD2, and the endothelial glycocalyx. These activities have been linked to immune evasion, vascular permeability, and protective immunity.
• Structurally, mature NS1 comprises 352 amino acids folded into a hydrophobic β-roll, an α/β wing, and a β-ladder domain, stabilized by 12 invariant cysteines forming six intramolecular disulfide bonds, with two conserved N-glycosylation sites.
• NS1 is an obligate homodimer, and this quaternary structure together with its glycosylation and disulfide bonding underlies the conformational epitopes recognized by most NS1-directed antibodies.

This product contains 7x His tag at C-terminus of protein.

Lyophilized

44-54 kDa

This recombinant protein is aseptically packaged (0.2um filtered) and formulated in 0.01 M PBS (phosphate buffered saline) (150 mM NaCl) pH 7.0 - 7.5 with no carrier protein, potassium, calcium or preservatives added before lyophilization.

Reconstitute at 0.1-1 mg/ml using filtered deionized water. Gently mix by vortexing and/or inversion until fully dissolved. Centrifuge if necessary.

This lyophilized protein is stable for twelve months when stored at -20°C to -70°C. After aseptic reconstitution, this protein may be stored for one month at 2°C to 8°C or for three months at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Ambient

Research Use Only

1. Muller, D. et al. (2012) J. Gen. Virol. 93:771.
2. VanBlargan, L.A. et al. (2013) PLoS Pathog. 9:e1003761.
3. Flamand, M. et al. (1999) J. Virol. 73:6104.