Recombinant Human CD137L (4-1BBL)

4-1BB ligand (4-1BBL), also known as TNFSF9 and CD137L, is a type 2 transmembrane glycoprotein belonging to the TNF superfamily. It is a ligand for TNFRSF9, which is a co-stimulatory molecule in T lymphocytes. 4-1BBL and its receptor are involved in the antigen presentation process, generation of cytotoxic T cells and are required for the optimal CD8 responses in CD8 T cells (1). It is expressed on activated T and B cells, macrophages, dendritic cells, neurons and astrocytes (2-3). 4-1BBL also plays an important role in co-stimulation of IL-2 production (3). In addition, 4-1BBL is expressed in various carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction (4). One study reports the long-term remission of liver metastases in a mouse model involving the gene transfer of IL-12 and 4-1BBL (5).

The biological activity of Human 4-1BBL was determined by the dose-dependent induction of IL-8 by human PBMCs. The expected ED₅₀ for this effect is 10 ng/ml.

Histidine Tag

hhhhhhregp elspddpagl ldlrqgmfaq lvaqnvllid gplswysdpg lagvsltggl sykedtkelv vakagvyyvf fqlelrrvva gegsgsvsla lhlqplrsaa gaaalaltvd lppassearn safgfqgrll hlsagqrlgv hlhteararh awqltqgatv lglfrvtpei paglpsprse

Met

Lyophilized

The predicted molecular weight of Recombinant Human 4-1BBL is Mr 20.4 kDa.

20.4

This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) and NaCl; pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.

This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution and addition of a carrier protein (such as 0.1% BSA or HSA) this protein may be stored at -20°C to -70°C for up to 3 months in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.

Next Day Ambient

1. Watts, TH. et al. (2002) J. Immunol. 168:4897
2. Watts, TH. et al. (1995) J. Exp. Med. 181:985
3. Lotze, M. et al. (2001) Dendritic Cells: Biology and Clinical Applications (Academic Press)
4. Tan, JT. et al. (1999) J. Immunol. 163:4859
5. Martinet, O. et al. (2000) J. Natl. Cancer Inst. 92:931