Recombinant Human 4-1BBL (CD137)

Pricing & Details

Product. No.F131
Alternate Names
4-1BB Ligand, TNFSF9, CD137L
Expression Host
E. coli
Reactivity Species
Human
Prod No.
Size
Price
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Qty
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F131-25 µg
25 µg
$279.00
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Min: 1
Step: 1

Background

4-1BB ligand (4-1BBL), also known as TNFSF9 and CD137L, is a type 2 transmembrane glycoprotein belonging to the TNF superfamily. It is a ligand for TNFRSF9, which is a co-stimulatory molecule in T lymphocytes. 4-1BBL and its receptor are involved in the antigen presentation process, generation of cytotoxic T cells and are required for the optimal CD8 responses in CD8 T cells (1). It is expressed on activated T and B cells, macrophages, dendritic cells, neurons and astrocytes (2-3). 4-1BBL also plays an important role in co-stimulation of IL-2 production (3). In addition, 4-1BBL is expressed in various carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction (4). One study reports the long-term remission of liver metastases in a mouse model involving the gene transfer of IL-12 and 4-1BBL (5).

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human 4-1BBL was determined by the dose-dependent induction of IL-8 by human PBMCs. The expected ED50 for this effect is 10 ng/ml.
Fusion Protein Tag
Histidine Tag
Expression Host
E. coli
Protein Accession No.
Amino Acid Sequence
hhhhhhregp elspddpagl ldlrqgmfaq lvaqnvllid gplswysdpg lagvsltggl sykedtkelv vakagvyyvf fqlelrrvva gegsgsvsla lhlqplrsaa gaaalaltvd lppassearn safgfqgrll hlsagqrlgv hlhteararh awqltqgatv lglfrvtpei paglpsprse
N-terminal Sequence Analysis
Met
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human 4-1BBL is Mr 20.4 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) and NaCl; pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution and addition of a carrier protein (such as 0.1% BSA or HSA) this protein may be stored at -20°C to -70°C for up to 3 months in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Watts, TH. et al. (2002) J. Immunol. 168:4897
2. Watts, TH. et al. (1995) J. Exp. Med. 181:985
3. Lotze, M. et al. (2001) Dendritic Cells: Biology and Clinical Applications (Academic Press)
4. Tan, JT. et al. (1999) J. Immunol. 163:4859
5. Martinet, O. et al. (2000) J. Natl. Cancer Inst. 92:931
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Prod No.
Description
F131
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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