Human CT-1 is a member of the IL-6 family of cytokines which also includes LIF, CNTF, OSM (Oncostatin M), IL-11, IL-6 and possibly NT-1/ BSF-3. CT-1 is a pleiotropic cytokine which signals through the LIF receptor and the gp130 receptor subunit. CT-1 has the ability to induce cardiac myocyte hypertrophy, and enhances the survival of cardiomyocyte and different neuronal populations. Biologically active human CT-1 is synthesized as a 201 amino acid polypeptide lacking a hydrophobic N-terminal secretion signal sequence. Human and mouse CT-1 share 80% aa sequence identity and exhibit cross-species activity.
>95% by SDS-PAGE and analyzed by silver stain.
<0.01 EU/µg as determined by the LAL method
The biological activity of Human CT-1 was determined in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1 (Kitamura, T. et al., 1989, J. Cell Physiol. 140:323). The expected ED50 is typically 1 - 4 ng/ml.
Protein Accession No.
Amino Acid Sequence
srregsled pqtdssvsll phleakirqt hslahlltky aeqllqeyvq lqgdpfglps fspprlpvag lsapapshag lpvherlrld aaalaalppl ldavcrrqae lnpraprllr rledaarqar algaaveall aalgaanrgp raeppaatas aasatgvfpa kvlglrvcgl yrewlsrteg dlgqllpggs a
N-terminal Sequence Analysis
State of Matter
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human CT-1 is Mr 21 kDa.
This recombinant protein was 0.2 µm filtered and is supplied in a solution of 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This protein is stable for twelve months when stored at -20°C to -70°C. After thawing and addition of a carrier protein, this protein may be stored at 2°C to 8°C for one month or for long term storage aliquot and freeze at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
NCBI Gene Bank
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.