Recombinant Human CD30 Ligand

Home/Recombinant Proteins/Recombinant Human CD30 Ligand

Pricing & Details

Product. No.C1334
Alternate Names
TNFSF8, CD153
Expression Host
NS0 cells
Species
Human
Prod No.
Size
Price
Avail.
Qty
Add to cart
C1334-50 µg
50 µg
$249.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Background

The CD30 ligand (CD30L), also known as CD153 and TNFSF8, is a type II transmembrane glycoprotein of the tumor necrosis factor ligand superfamily (1). CD30L is expressed on the cell surface of activated T cells, B cells, stimulated monocyte-macrophages, granulocytes, eosinophils and some Burkitt-like lymphoma cell lines (2). Recombinant CD30L has been shown to co-stimulate T cells and to act as a mitogen for Hodgkin's disease derived cell lines (3). The receptor for CD30L is a type I cytokine receptor that is highly expressed by activated T cells, Hodgkin and Reed- Sternberg (H-RS) cells, and anaplastic large cell lymphoma cells (4). CD30 ligation by CD30L mediates pleiotropic effects including cell proliferation, activation, differentiation and cell death by apoptosis (5).

Protein Details

Purity
>95% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human CD30L was determined by its ability to stimulate IL-6 secretion by HDLM cells, a Hodgkin’s lymphoma cell line (Gruss, H-J. et al., 1996, Blood 87:2443). The expected ED50 for this effect is typically 0.1 - 0.4 μg/mL in the presence of 10 μg/mL of a cross-linking antibody PN:R1251.
Fusion Protein Tag
Fc Fusion Protein
Expression Host
NS0 cells
Protein Accession No.
Amino Acid Sequence
mhhhhhhhhh hgggsgggsg ggsiegrqrt dsipnspdnv plkggncsed llcilkrapf kkswaylqva khlnktklsw nkdgilhgvr yqdgnlviqf pglyfiicql qflvqcpnns vdlklellin khikkqalvt vcesgmqtkh vyqnlsqfll dylqvnttis vnvdtfqyid tstfplenvl siflysnsd
N-terminal Sequence Analysis
Met
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human CD30 Ligand is Mr 22 kDa. However, the actual molecular weight as observed by migration on SDS-PAGE is Mr 30-40 kDa.
Formulation
This recombinant protein was 0.2 µm filtered and lyophilized from modified Dulbecco’s phosphate buffered saline (1X PBS) pH 7.2 – 7.3 with no calcium, magnesium, or preservatives.
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Croager, EJ. et al. (1997) Biochim. Biophys. Acta. 1353:231
2. Gruss, HJ. et al. (1994) Blood 83:1521
3. Wendtner, CM. et al. (1995) Cancer Res. 55:4157
4. Fischer, M. et al. (2006) J. Clin. Invest. 116:2748
5. Blazar, BR. et al. (2004) J. Immunol. 173:2933
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
Leinco Technologies uses cookies to improve your experience and our website service. By continuing to browse our website, you accept our cookie policy. Ok