Recombinant Human CXCL2

Recombinant Human CXCL2

Product No.: G131

[product_table name="All Top" skus="G131"]

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Alternate Names
Chemokine (C-X-C Motif) Ligand 2 SCYB2, GRO2, Growth-Regulated Protein Beta (GROb), MIP-2a, MGSA-b, CINC-2a, MIP-2, Cytokine-Induced Neutrophil Chemotactic Factor 3 (CINC-3), gro beta, groβ,
Product Type
Recombinant Protein
Expression Host
E. coli Cells
Species
Human

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Select Product Size
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Background

Chemokine (C-X-C motif) ligand 2 (CXCL2), also known as MIP2-α and GROβ, is a small, inducible cytokine belonging to the CXC chemokine family. Other chemokines in this group include IL-8, GROα, GROγ, mouse KC, ENA78, GCP2 and PBP/CTAPIII/β TG/NAP2. Since it is structurally related to IL-8, CXCL2 competes with IL-8 for binding to IL-8 receptors on neutrophils and to a lesser extent, basophils. CXCL2 is involved in attraction of polymorphonuclear granulocytes to sites of infection (1). It is active against Gram-negative (Escherichia coli) and Gram-positive (Staphyllococcus aureus) bacteria. It is secreted by monocytes and macrophages and is chemotactic for hematopoietic stem cells (2-4). MMP-12 cleaves CXCL2 at the ELR sequence motif, which is known to be the critical receptor binding motif leading to the loss of chemotactic activity (5).

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human GRO-Beta was determined by by its ability to induce myeloperoxidase release from cytochalasin B treated neutrophils (Schröeder, J. et al., 1987, J. Immunol. 139:3474), or chemotaxis of hCXCR2 transfected mouse BaF/3 cells. The expected ED<sub>50</sub> for these effects are typically 0.3 - 0.9 μg/ml, or 3 - 15 ng/ml, respectively.
Protein Accession No.
Amino Acid Sequence
aplate lrcqclqtlq gihlkniqsv kvkspgphca qteviatlkn gqkaclnpas pmvkkiiekm lkngksn
N-terminal Sequence Analysis
Ala35
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human CXCL2 is Mr 8 kDa.
Predicted Molecular Mass
8
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
NCBI Gene Bank

References & Citations

1. Beuscher, HU. et al. (2004) International Immunol. 16: 1675
2. Cerami, A. et al. (1989) Proc Nat Acad Sci. 86: 612
3. Grotendorst, GR. et al. (1990) Mol Cell Biol. 10: 5596
4. Fukuda, S. et al. (2006) Exp Hematol. 34: 1010

Certificate of Analysis

IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Disclaimer AlertProducts are for research use only. Not for use in diagnostic or therapeutic procedures.