Recombinant Human CXCL2

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Pricing & Details

Product. No.G131
Alternate Names
Chemokine (C-X-C Motif) Ligand 2 SCYB2, GRO2, Growth-Regulated Protein Beta (GROb), MIP-2a, MGSA-b, CINC-2a, MIP-2, Cytokine-Induced Neutrophil Chemotactic Factor 3 (CINC-3), gro beta, groβ,
Expression Host
E. coli
Species
Human
Prod No.
Size
Price
Avail.
Qty
Add to cart
G131-10 µg
10 µg
$219.00
In stock
Max:
Min: 1
Step: 1
G131-50 µg
50 µg
$599.00
In stock
Max:
Min: 1
Step: 1
Bulk quantities available. Contact us for pricing.

Background

Chemokine (C-X-C motif) ligand 2 (CXCL2), also known as MIP2-α and GROβ, is a small, inducible cytokine belonging to the CXC chemokine family. Other chemokines in this group include IL-8, GROα, GROγ, mouse KC, ENA78, GCP2 and PBP/CTAPIII/β TG/NAP2. Since it is structurally related to IL-8, CXCL2 competes with IL-8 for binding to IL-8 receptors on neutrophils and to a lesser extent, basophils. CXCL2 is involved in attraction of polymorphonuclear granulocytes to sites of infection (1). It is active against Gram-negative (Escherichia coli) and Gram-positive (Staphyllococcus aureus) bacteria. It is secreted by monocytes and macrophages and is chemotactic for hematopoietic stem cells (2-4). MMP-12 cleaves CXCL2 at the ELR sequence motif, which is known to be the critical receptor binding motif leading to the loss of chemotactic activity (5).

Protein Details

Purity
>97% by SDS-PAGE and analyzed by silver stain.
Endotoxin Level
<0.1 EU/µg as determined by the LAL method
Biological Activity
The biological activity of Human GRO-Beta was determined by by its ability to induce myeloperoxidase release from cytochalasin B treated neutrophils (Schröeder, J. et al., 1987, J. Immunol. 139:3474), or chemotaxis of hCXCR2 transfected mouse BaF/3 cells. The expected ED50 for these effects are typically 0.3 - 0.9 μg/ml, or 3 - 15 ng/ml, respectively.
Expression Host
E. coli
Protein Accession No.
Amino Acid Sequence
aplate lrcqclqtlq gihlkniqsv kvkspgphca qteviatlkn gqkaclnpas pmvkkiiekm lkngksn
N-terminal Sequence Analysis
Ala35
State of Matter
Lyophilized
Predicted Molecular Mass
The predicted molecular weight of Recombinant Human CXCL2 is Mr 8 kDa.
Formulation
This recombinant protein was lyophilized from a 0.2 μm filtered solution in 35% acetonitrile (CH3CN) and 0.1% trifluoroacetic acid (TFA).
Storage and Stability
This lyophilized protein is stable for six to twelve months when stored desiccated at -20°C to -70°C. After aseptic reconstitution, this protein may be stored at 2°C to 8°C for one month or at -20°C to -70°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles. See Product Insert for exact lot specific storage instructions.
Country of Origin
USA
Shipping
Next Day Ambient
PubMed
NCBI Gene Bank

References & Citations

1. Beuscher, HU. et al. (2004) International Immunol. 16: 1675
2. Cerami, A. et al. (1989) Proc Nat Acad Sci. 86: 612
3. Grotendorst, GR. et al. (1990) Mol Cell Biol. 10: 5596
4. Fukuda, S. et al. (2006) Exp Hematol. 34: 1010
IMPORTANT Use lot specific datasheet for all technical information pertaining to this recombinant protein.
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
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