Anti-Mouse CXCL2 (Clone 40605)
Anti-Mouse CXCL2 (Clone 40605)
Product No.: M1277
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Clone 40605 Target CXCL2 Formats AvailableView All Product Type Monoclonal Antibody Alternate Names SCYB2, GRO2, Growth-Regulated Protein Beta (GROb), MIP-2a, MGSA-b, CINC-2a, MIP-2, Cytokine-Induced Neutrophil Chemotactic Factor 3 (CINC-3), Chemokine (C-X-C Motif) Ligand 2 Isotype IgG2b Applications ELISA Cap , N , WB |
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Antibody DetailsProduct DetailsReactive Species Mouse Host Species Rat Immunogen Purified Recombinant Mouse CXCL2 (>98%) Endotoxin Level <0.1 EU/µg as determined by the LAL method Formulation This monoclonal antibody has been 0.2 µm filtered and lyophilized from modified Dulbecco's phosphate buffered saline (1X PBS) pH 7.2 - 7.4 containing 5.0% w/v trehalose with no calcium, magnesium or preservatives present. Storage and Handling The lyophilized antibody can be stored desiccated at -20°C to -70°C for up to twelve months. The reconstituted antibody can be stored for at least four weeks at 2-8°C. For long-term storage of the reconstituted antibody, aseptically aliquot into working volumes and store at -20°C to -70°C in a manual defrost freezer. Avoid repeated freeze thaw cycles. No detectable loss of activity was observed after six months. Country of Origin USA Shipping Next Day Ambient RRIDAB_2831271 Applications and Recommended Usage? Quality Tested by Leinco ELISA Sandwich: This antibody is useful as the capture antibody in a sandwich ELISA. The suggested coating concentration is 0.1-0.4 µg/ml. A suitable detection antibody is PN:M195 at a concentration of approximately 2.0 µg/ml. When used in conjunction with an optimal second step reagent such as PN:A106 and a chromogenic substrate such as PN:T118, the sensitivity of this sandwich ELISA is 2 ng/well. A suggested standard for this assay is PN:M174. Western Blotting: To detect Mouse CXCL2 this monoclonal antibody can be used at a concentration of 1 - 2 µg/ml. This monoclonal antibody should be used in conjunction with compatible second-step reagents such as PN:R377 and a chromogenic substrate such as PN:T263. The detection limit for Mouse CXCL2 is 5 ng/lane under either reducing or non-reducing conditions. The sensitivity of detection may increase up to 50 fold when a chemiluminescent substrate is used. A suitable Western blotting control is PN:M174. Additional Applications Reported In Literature ? Neutralization: This antibody is useful for neutralization of Mouse MIP-2 bioactivity. The antibody dose required to neutralize 50% (ND50) of the biological activity of Mouse MIP-2 (at 2 ng/ml) is 1-4 µg/ml. Each investigator should determine their own optimal working dilution for specific applications. See directions on lot specific datasheets, as information may periodically change. DescriptionSpecificity Rat Anti-Mouse Chemokine (C-X-C Motif) Ligand 2 (CXCL2) (Clone 40605) recognizes an epitope on Mouse CXCL2. This monoclonal antibody was purified using multi-step affinity chromatography methods such as Protein A or G depending on the species and isotype. Background Chemokine (C-X-C motif) ligand 2 (CXCL2) is an inducible murine chemokine involved in attraction of polymorphonuclear granulocytes to sites of infection.1 This chemokine is secreted by monocytes and macrophages and is chemotactic for hematopoietic stem cells.2,3,4 Antigen DetailsPubMed References & Citations1. Beuscher, HU. et al. (2004) International Immunol. 16: 1675 2. Cerami, A. et al. (1989) Proc Nat Acad Sci. 86: 612 3. Grotendorst, GR. et al. (1990) Mol Cell Biol. 10: 5596 4. Fukuda, S. et al. (2006) Exp Hematol. 34: 1010 Technical Protocols N  |
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Formats Available
Products are for research use only. Not for use in diagnostic or therapeutic procedures.
